Array of Single-cell Pairs on a Microwell Array Based on Positive Dielectrophoresis

Yoshimura, Yuki; Fujii, Chiaki; Tomita, Masahiro; Mizutani, Fumio; Yasukawa, Tomoyuki

doi:10.1246/cl.140195

Cited by 9 publications

(5 citation statements)

References 15 publications

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“…Previously, we developed a rapid and simple fabrication method of cell array by using microwell array electrodes based on the attractive force of positive DEP (p-DEP). 9,10 Pairs of different types of cells in individual microwells were also formed to apply to the fabrication of hybrid cells by cell fusion. By using negative DEP (n-DEP), patterns with cells and particles can be fabricated on a substrate opposite to a substrate with an electrode pattern.…”

Section: Introductionmentioning

confidence: 99%

Rapid Formation of Aggregates with Uniform Numbers of Cells Based on Three-dimensional Dielectrophoresis

et al. 2019

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We applied a fabrication method for the formation of island organization of cells based on a three-dimensional (3D) device for negative dielectrophoresis (n-DEP) to produce cell aggregates with uniform numbers of cells rapidly and simply. The intersections formed by rotating the interdigitated array (IDA) with two combs of band electrodes on the upper substrate by 90 relative to the IDA with two combs on the lower substrate were prepared in the device. The AC voltage was applied to a comb on the upper substrate and a comb on the lower substrate, while AC voltage with opposite phase was applied to another comb on the upper substrate and another comb on the lower substrate. Cells dispersed randomly were directed toward the intersections with relatively lower electric fields due to n-DEP, which formed by AC voltage applied bands with the identical phase, resulting in the formation of island patterns of cells. The cells accumulated at intersections were promoted to form the cell aggregates due to the close contact together. The production of cell aggregations adhered together was easily found by the dispersion behavior after switching the applied frequency to convert the cellular pattern. When cells were accumulated at the intersections by n-DEP for 45 min, almost accumulations of cells were adhered together, and hence a formations of cell aggregations. By using the present method, we can rapidly and simply fabricate cell aggregations with a uniform number of cells.

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Section: Introductionmentioning

confidence: 99%

Rapid Formation of Aggregates with Uniform Numbers of Cells Based on Three-dimensional Dielectrophoresis

et al. 2019

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“…We have fabricated a microwell array electrode to introduce individual cells to microwells by p-DEP. (27) The device with microwell arrays was applied to form pairs of different types of cells. (28) More recently, gold nanohole arrays were combined with particle manipulation in order to enhance the performance of surface plasmon resonance sensors in detecting biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Particle Patterning Based on Positive Dielectrophoresis Using a Scanning Microelectrode

Yasukawa¹,

Gotoh²,

Yasuda³

et al. 2019

Sensors and Materials

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Positioning and patterning of polystyrene particles on a silicon nitride (SiN) membrane with a microhole array has been demonstrated by positive dielectrophoresis (p-DEP). A chamber with an SiN membrane with the well-aligned microholes as the bottom substrate was positioned on an indium-tin-oxide (ITO) electrode with a 1 mm space between the bottom substrate and the ITO electrode. The chamber and the space were filled with water and a suspension of particles (10 µm diameter) in water, respectively. An AC electric signal was then applied to a microelectrode positioned at 10 µm above the SiN membrane, while the ITO electrode was connected to the ground. Particles present in the space between the SiN membrane and ITO electrode gradually moved toward the lower surface of the SiN membrane directly under the microelectrode owing to the strong electric field generated on and in localized microholes and accumulated at this position to form aggregates. For particles of 3 µm diameter, one particle was deposited in each hole (2 µm diameter) in the region directly under the microelectrode. The horizontal movement of the microelectrode gave rise to the formation of a line pattern of particles along the trail of the microelectrode because of the shift of the region with the strong electric field. These demonstrations could be applicable to arranging the particles at desired positions and in desired holes, and form particle patterns with highly flexible designs.

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“…[25][26][27][28][29] We have previously demonstrated the trapping of cells in microwells at the single-cell level within a few seconds with an occupation efficiency of 95% using a microwell array device. [29][30][31] However, it has not been possible to regulate the selective manipulation to retrieve certain target cells from the cell array and retain single cells in microwells by removing other cells because the electric fields generated in each microwell cannot be individually controlled. Currently, the recovery of the target cells after determining the cellular function of individual cells trapped in a cell array employs the suction of the target cell in microwell by a microdispenser.…”

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confidence: 99%

Selective Trapping and Retrieval of Single Cells Using Microwell Array Devices Combined with Dielectrophoresis

2021

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Array of Single-cell Pairs on a Microwell Array Based on Positive Dielectrophoresis

Cited by 9 publications

References 15 publications

Rapid Formation of Aggregates with Uniform Numbers of Cells Based on Three-dimensional Dielectrophoresis

Rapid Formation of Aggregates with Uniform Numbers of Cells Based on Three-dimensional Dielectrophoresis

Particle Patterning Based on Positive Dielectrophoresis Using a Scanning Microelectrode

Selective Trapping and Retrieval of Single Cells Using Microwell Array Devices Combined with Dielectrophoresis

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