Aromatic-turmerone inhibits α-MSH and IBMX-induced melanogenesis by inactivating CREB and MITF signaling pathways

Abstract

This study investigated the anti-melanogenic effect of aromatic (ar)-turmerone on alpha-melanocyte stimulating hormone (α-MSH) and 3-isobuty-1-methxlzanthine (IBMX)-induced tyrosinase (Tyr), tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) expression in B16F10 melanoma cells. We demonstrated that ar-turmerone inhibits α-MSH and IBMX-induced melanin synthesis and tyrosinase activity. Data also showed that ar-turmerone inhibits the expression of tyrosinase, TRP-1, and TRP-2 in α-MSH- and IBMX-stimulated B16F10 cells. In addition, ar-turmerone exhibits stronger anti-melanogenic effects than curcumin. Furthermore, ar-turmerone strongly inhibited α-MSH- and IBMX-induced microphthalmia-associated transcription factor by suppressing the activity of cyclic adenosine monophosphate (cAMP)-responsive element binding protein in α-MSH-stimulated B16F10 cells. Our data revealed that ar-turmerone is a novel, effective, anti-melanogenic agent that functions by downregulating tyrosinase, Trp-1, and Trp-2 gene expression. Therefore, ar-turmerone may be a useful therapeutic agent for treating hyperpigmentation disorders, such as freckles and melasma, and as a beneficial additive in whitening cosmetics.

Optimization of extraction conditions for osthol, a melanogenesis inhibitor fromCnidium monnierifruits

“…As shown in Figure 3(A), osthol showed little effect on tyrosinase activity. Melanin synthesis are known to be regulated by melanogenic enzymes such as the tyrosinase-related protein 1 (TRP-1) and TRP-2 as well as tyrosinase (Nakashima et al, 2010;Park et al, 2011). Therefore, the effect of osthol on the expressions of TRP-1, TRP-2, and tyrosinase was determined.…”

Maturation and upregulation of functions of murine dendritic cells (DCs) under the influence of purified Aromatic-Turmerone (AR)

“…20 Moreover, AR can be employed as an agent in functional cosmetics to develop safe and effective skin-whitening treatments. 21 The AR also possessed anti-oxidant 22 and antiplatelet activities. 23,24 Now our study provided findings with following major fact: (1) turmeric could remarkably induce the morphological maturation of BMDCs, that was that the matured BMDCs showed a more irregular external surface with more cytoplasmic projection (Figs.…”

Lipoteichoic Acid Isolated from Lactobacillus plantarum Inhibits Melanogenesis in B16F10 Mouse Melanoma Cells

“…The tyrosinase gene family responsible for the regulation of melanogenesis consists of tyrosinase, TRP-1, and TRP-2. Tyrosinase is a key enzyme involved in melanogenesis and catalyzes the hydroxylation of L-tyrosinase to DOPA and the oxidation of DOPA to DOPA quinone (Koo et al, 2010;Park et al, 2011). TRP-1 and TRP-2 are enzymes that belong to the tyrosinase family and regulate melanogenesis.…”