Arhodomonas recens sp. nov., a halophilic alkane-utilizing hydrogen-oxidizing bacterium from the brines of flotation enrichment of potassium minerals

Saralov, A. I.; Кузнецов, Б. Б.; Reutskikh, E. M.; Baslerov, R. V.; Panteleeva, A. N.; Suzina, N. E.

doi:10.1134/s002626171205013x

Cited by 17 publications

(5 citation statements)

References 9 publications

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“…Two gammaproteobacterial strains that were isolated from the white brine are potential novel species having a sequence identity less than 96% with known species. One isolate has 95.2% identity with Arhodomonas recens RS91, a halophilic alkane-utilizing hydrogen-oxidizing bacterium 42 and the other has 95.7% identity with Spiribacter salinus M19–40, an abundant halophilic bacterium isolated from 190 g kg −1 ponds of salterns on Isla Christina, Spain 43 . Among the haloarchaea, genus Halalkalicoccus , Halobiforma had a higher relative abundance in the red brine while genera like Haloquadratum were more abundant in the high salinity brine.…”

Section: Discussionmentioning

confidence: 99%

Microbial diversity in the hypersaline Lake Meyghan, Iran

Naghoni

Emtiazi

Amoozegar

et al. 2017

Sci Rep

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Lake Meyghan is one of the largest and commercially most important salt lakes in Iran. Despite its inland location and high altitude, Lake Meyghan has a thalassohaline salt composition suggesting a marine origin. Inputs of fresh water by rivers and rainfall formed various basins characterized by different salinities. We analyzed the microbial community composition of three basins by isolation and culturing of microorganisms and by analysis of the metagenome. The basins that were investigated comprised a green ~50 g kg−1 salinity brine, a red ~180 g kg−1 salinity brine and a white ~300 g kg−1 salinity brine. Using different growth media, 57 strains of Bacteria and 48 strains of Archaea were isolated. Two bacterial isolates represent potential novel species with less than 96% 16S rRNA gene sequence identity to known species. Abundant isolates were also well represented in the metagenome. Bacteria dominated the low salinity brine, with Alteromonadales (Gammaproteobacteria) as a particularly important taxon, whereas the high salinity brines were dominated by haloarchaea. Although the brines of Lake Meyghan differ in geochemical composition, their ecosystem function appears largely conserved amongst each other while being driven by different microbial communities.

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Section: Discussionmentioning

confidence: 99%

Microbial diversity in the hypersaline Lake Meyghan, Iran

Naghoni

Emtiazi

Amoozegar

et al. 2017

Sci Rep

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“…The strain formed a tight clade with Arhodomonas sp. strain Rozel, isolated in our laboratory from Rozel Point, UT (42), Arhodomonas aquaeolei strain HA-1 isolated from petroleum reservoir production fluid from Oklahoma (47), and Arhodomonas recens strain RS91, isolated from acidic brines of flotation enrichment from potassium minerals from Silvinit Co., Solikamsk, Russia (48). The strain shares 98, 97, and 96% sequence similarity, respectively, with Arhodomonas sp.…”

Section: Resultsmentioning

confidence: 99%

Arhodomonas sp. Strain Seminole and Its Genetic Potential To Degrade Aromatic Compounds under High-Salinity Conditions

Dalvi

Nicholson

Najar

et al. 2014

Appl Environ Microbiol

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cArhodomonas sp. strain Seminole was isolated from a crude oil-impacted brine soil and shown to degrade benzene, toluene, phenol, 4-hydroxybenzoic acid (4-HBA), protocatechuic acid (PCA), and phenylacetic acid (PAA) as the sole sources of carbon at high salinity. Seminole is a member of the genus Arhodomonas in the class Gammaproteobacteria, sharing 96% 16S rRNA gene sequence similarity with Arhodomonas aquaeolei HA-1. Analysis of the genome predicted a number of catabolic genes for the metabolism of benzene, toluene, 4-HBA, and PAA. The predicted pathways were corroborated by identification of enzymes present in the cytosolic proteomes of cells grown on aromatic compounds using liquid chromatography-mass spectrometry. Genome analysis predicted a cluster of 19 genes necessary for the breakdown of benzene or toluene to acetyl coenzyme A (acetyl-CoA) and pyruvate. Of these, 12 enzymes were identified in the proteome of toluene-grown cells compared to lactate-grown cells. Genomic analysis predicted 11 genes required for 4-HBA degradation to form the tricarboxylic acid (TCA) cycle intermediates. Of these, proteomic analysis of 4-HBA-grown cells identified 6 key enzymes involved in the 4-HBA degradation pathway. Similarly, 15 genes needed for the degradation of PAA to the TCA cycle intermediates were predicted. Of these, 9 enzymes of the PAA degradation pathway were identified only in PAA-grown cells and not in lactate-grown cells. Overall, we were able to reconstruct catabolic steps for the breakdown of a variety of aromatic compounds in an extreme halophile, strain Seminole. Such knowledge is important for understanding the role of Arhodomonas spp. in the natural attenuation of hydrocarbon-impacted hypersaline environments.

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“…Numerous haloarchaeal species, including Arhodomonas sp., Haloarchaeum sp. and Haloferax sp., have been discovered from a flotation enrichment step and mineral samples from a Russian potassium mining company [ 57 , 58 , 59 ]. And Halobacterium sp., Haloarcula sp.…”

Section: Discussionmentioning

confidence: 99%

Different Responses of Bacteria and Archaea to Environmental Variables in Brines of the Mahai Potash Mine, Qinghai-Tibet Plateau

Xie

et al. 2023

Microorganisms

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Salt mines feature both autochthonous and allochthonous microbial communities introduced by industrialization. It is important to generate the information on the diversity of the microbial communities present in the salt mines and how they are shaped by the environment representing ecological diversification. Brine from Mahai potash mine (Qianghai, China), an extreme hypersaline environment, is used to produce potash salts for hundreds of millions of people. However, halophiles preserved in this niche during deposition are still unknown. In this study, using high-throughput 16S rRNA gene amplicon sequencing and estimation of physicochemical variables, we examined brine samples collected from locations with the gradient of industrial activity intensity and discrete hydrochemical compositions in the Mahai potash mine. Our findings revealed a highly diverse bacterial community, mainly composed of Pseudomonadota in the hypersaline brines from the industrial area, whereas in the natural brine collected from the upstream Mahai salt lake, most of the 16S rRNA gene reads were assigned to Bacteroidota. Halobacteria and halophilic methanogens dominated archaeal populations. Furthermore, we discovered that in the Mahai potash mining area, bacterial communities tended to respond to anthropogenic influences. In contrast, archaeal diversity and compositions were primarily shaped by the chemical properties of the hypersaline brines. Conspicuously, distinct methanogenic communities were discovered in sets of samples with varying ionic compositions, indicating their strong sensitivity to the brine hydrochemical alterations. Our findings provide the first taxonomic snapshot of microbial communities from the Mahai potash mine and reveal the different responses of bacteria and archaea to environmental variations in this high-altitude aquatic ecosystem.

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Arhodomonas recens sp. nov., a halophilic alkane-utilizing hydrogen-oxidizing bacterium from the brines of flotation enrichment of potassium minerals

Cited by 17 publications

References 9 publications

Microbial diversity in the hypersaline Lake Meyghan, Iran

Microbial diversity in the hypersaline Lake Meyghan, Iran

Arhodomonas sp. Strain Seminole and Its Genetic Potential To Degrade Aromatic Compounds under High-Salinity Conditions

Different Responses of Bacteria and Archaea to Environmental Variables in Brines of the Mahai Potash Mine, Qinghai-Tibet Plateau

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