Argyrophilic Nucleoar Organizer Regions: An Improved Technique for Visualization

McLemore, Donia; Lord, Barbara A.

doi:10.1179/his.1991.14.3.187

Cited by 12 publications

(4 citation statements)

References 18 publications

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“…PH has been suggested as an important variable in the gelatin solution, with a report that raising the pH of this solution to 3 eliminated precipitates (22). We confirmed that reducing the p H dramatically increased precipitates with a lot of gelatin that produced precipitates and raising it decreased them.…”

Section: VIsupporting

confidence: 75%

“…Several authors have proposed variations in the original procedure to reduce these problems, including: destaining of slides before silver staining (2); pre-or post-incubation of sections with alcoholic acetic acid (3,4,8,10,28), other acids (7,21), or glycine (7) to reduce background staining; variations in solution concentration (54); increase in pH (22); variations in temperature (3,4,7, 439 9,12,17); staining slides while inverted (4) or coating the sections with collodion ( 3 ) to reduce precipitates; conversion of the silver precipitate to a dye complex (12); combination with other staining procedures (18); replacement of the gelatin component with other colloids (26); and use of sodium thiosulfate (8,9,12) and gold toning (8,22) to improve permanence. None of these modifications has entirely controlled the problems.…”

Section: Introductionmentioning

confidence: 99%

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Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR).

Lindner

1993

J Histochem Cytochem.

103

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The silver-staining technique for nucleolar organizer regions (AgNOR) of Ploton et al., as popularized by Crocker, is being widely used for evaluation of nucleolar function, especially in neoplasia. It suffers from limited reliability, background staining, precipitates, and fading of the sections. Factors were identified that affect these problems. The oxidation-reduction level and gelatin used are particularly important. An improved procedure is presented which incor-

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Section: VIsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR).

Lindner

1993

J Histochem Cytochem.

103

View full text Add to dashboard Cite

show abstract

“…The slides were then washed in deionized, distilled water and exposed for 45 min to a freshly prepared staining solution of two parts 50 per cent aqueous silver nitrate solution and one part of gelatin. The gelatin was previously prepared by dissolving 2.0 gm granular gelatin in 100 ml of 1.0 per cent formic acid.The gelatin solution was brought to an optimum pH of 3.0 with IN sodium hydroxide (McLemore and Lord, 1991). After incubation, the slides were washed with deionized distilled water and dehydrated through increasing concentrations of ethanol solution, cleared in xylene and coverslipped.…”

Section: Agnor Stainingmentioning

confidence: 99%

Nucleolar organizer regions (NORs) and myoepitheliomas: a comparison with DNA content and clinical course

Carrillo¹,

El‐Naggar²,

Luna³

et al. 1992

J. Laryngol. Otol.

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“…These NORs can be visualized by the argyrophilia of their associated proteins, using the so-called AgNOR technique [Crocker et al, 1989;McLemore and Lord, 1991]. The number of AgNORs (silver-stained nucleolar organizer regions) per nucleus was suggested to correlate with cellular activity [Crocker et al, 1989].…”

Section: Agnor Activitymentioning

confidence: 99%

Embryonic Blastemic Changes in Retinoic Acid-Induced Hindlimb Deformity

Santos-Álvarez¹,

Martos-Rodríguez²,

Delgado-Baeza³

2003

Cells Tissues Organs

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In this study we analyzed, on an experimental model, blastemic changes occurring during the embryonic period that may later cause fetal hindlimb deformity. Experimental induction of clubfoot-like deformity in rat fetuses was performed by maternal administration of retinoic acid (RA) (120 mg/kg body weight) as a single intragastric dose on day 10 of pregnancy (previous phase of this assay). The caudal and hindlimb blastemic changes were studied by mitosis count and stereological, immunohistochemistry and AgNOR techniques in the hindlimbs of 15-day embryos and in the caudal somites of 11-day embryos. In 15-day embryos from the assay group, hypoplasia and misorientation of hindlimbs were present in 90% of the cases. The histological study showed a blastemal defect as follows: (a) reduction in mesenchymal cell activity (mitotic and AgNOR activities); (b) increase of volume of vascular lumen; (c) reduction in volume of nerve structures, and (d) reduction in the percentage of pre-rhabdomyoblastic cells. In 11-day embryos from the assay group, caudal somites showed disruption, including loss of usual morphology. Moreover, somitic AgNOR activity decreased compared to the control group. The greatest reduction in the number of black-dots per cell was in the myotome. These findings suggest that a certain pathology of the somites, very little studied to date, might be involved in clubfoot pathogenesis.

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Argyrophilic Nucleoar Organizer Regions: An Improved Technique for Visualization

Cited by 12 publications

References 18 publications

Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR).

Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR).

Nucleolar organizer regions (NORs) and myoepitheliomas: a comparison with DNA content and clinical course

Embryonic Blastemic Changes in Retinoic Acid-Induced Hindlimb Deformity

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