1991
DOI: 10.1179/his.1991.14.3.187
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Argyrophilic Nucleoar Organizer Regions: An Improved Technique for Visualization

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Cited by 12 publications
(4 citation statements)
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“…PH has been suggested as an important variable in the gelatin solution, with a report that raising the pH of this solution to 3 eliminated precipitates (22). We confirmed that reducing the p H dramatically increased precipitates with a lot of gelatin that produced precipitates and raising it decreased them.…”
Section: VIsupporting
confidence: 75%
See 1 more Smart Citation
“…PH has been suggested as an important variable in the gelatin solution, with a report that raising the pH of this solution to 3 eliminated precipitates (22). We confirmed that reducing the p H dramatically increased precipitates with a lot of gelatin that produced precipitates and raising it decreased them.…”
Section: VIsupporting
confidence: 75%
“…Several authors have proposed variations in the original procedure to reduce these problems, including: destaining of slides before silver staining (2); pre-or post-incubation of sections with alcoholic acetic acid (3,4,8,10,28), other acids (7,21), or glycine (7) to reduce background staining; variations in solution concentration (54); increase in pH (22); variations in temperature (3,4,7, 439 9,12,17); staining slides while inverted (4) or coating the sections with collodion ( 3 ) to reduce precipitates; conversion of the silver precipitate to a dye complex (12); combination with other staining procedures (18); replacement of the gelatin component with other colloids (26); and use of sodium thiosulfate (8,9,12) and gold toning (8,22) to improve permanence. None of these modifications has entirely controlled the problems.…”
Section: Introductionmentioning
confidence: 99%
“…The slides were then washed in deionized, distilled water and exposed for 45 min to a freshly prepared staining solution of two parts 50 per cent aqueous silver nitrate solution and one part of gelatin. The gelatin was previously prepared by dissolving 2.0 gm granular gelatin in 100 ml of 1.0 per cent formic acid.The gelatin solution was brought to an optimum pH of 3.0 with IN sodium hydroxide (McLemore and Lord, 1991). After incubation, the slides were washed with deionized distilled water and dehydrated through increasing concentrations of ethanol solution, cleared in xylene and coverslipped.…”
Section: Agnor Stainingmentioning
confidence: 99%
“…These NORs can be visualized by the argyrophilia of their associated proteins, using the so-called AgNOR technique [Crocker et al, 1989;McLemore and Lord, 1991]. The number of AgNORs (silver-stained nucleolar organizer regions) per nucleus was suggested to correlate with cellular activity [Crocker et al, 1989].…”
Section: Agnor Activitymentioning
confidence: 99%