Argininic acid alters markers of cellular oxidative damage in vitro : Protective role of antioxidants

Lima, Daniela Delwing-de; Sasso, Simone; Dalmedico, Leticia; Magro, Débora Delwing‐Dal; Pereira, Eduardo Manoel; Wyse, Ângela T. S.

doi:10.1016/j.etp.2017.05.007

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…It is generally known that antioxidants play a protective role in defensing free-radical damage in PD [26]. Thus, we investigated the ROS level in the SN, the result showed that MPTP treatment increased the ROS level compared to the controls, and after hLf treatment, the levels of ROS were significantly decreased (p < 0.05, Fig.…”

Section: Resultsmentioning

confidence: 85%

Lactoferrin ameliorates dopaminergic neurodegeneration and motor deficits in MPTP-treated mice

Zhang

Wang

et al. 2019

Redox Biology

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Brain iron accumulation is common in patients with Parkinson's disease (PD). Iron chelators have been investigated for their ability to prevent neurodegenerative diseases with features of iron overload. Given the non-trivial side effects of classical iron chelators, lactoferrin (Lf), a multifunctional iron-binding globular glycoprotein, was screened to identify novel neuroprotective pathways against dopaminergic neuronal impairment. We found that Lf substantially ameliorated PD-like motor dysfunction in the subacute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD. We further showed that Lf could alleviate MPTP-triggered apoptosis of DA neurons, neuroinflammation, and histological alterations. As expected, we also found that Lf suppressed MPTP-induced excessive iron accumulation and the upregulation of divalent metal transporter (DMT1) and transferrin receptor (TFR), which is the main intracellular iron regulation protein, and subsequently improved the activity of several antioxidant enzymes. We probed further and determined that the neuroprotection provided by Lf was involved in the upregulated levels of brain-derived neurotrophic factor (BDNF), hypoxia-inducible factor 1α (HIF-1α) and its downstream protein, accompanied by the activation of extracellular regulated protein kinases (ERK) and cAMP response element binding protein (CREB), as well as decreased phosphorylation of c-Jun N-terminal kinase (JNK) and mitogen activated protein kinase (MAPK)/P38 kinase in vitro and in vivo. Our findings suggest that Lf may be an alternative safe drug in ameliorating MPTP-induced brain abnormalities and movement disorder.

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Section: Resultsmentioning

confidence: 85%

Lactoferrin ameliorates dopaminergic neurodegeneration and motor deficits in MPTP-treated mice

Zhang

Wang

et al. 2019

Redox Biology

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“…Similarly, there are several studies indicating that citrulline may have beneficial effects to the host (Chapman et al, 2012; Bahri et al, 2013; Breuillard et al, 2015; Antunes et al, 2016; Lai et al, 2017). In contrast, a study found that Arginic Acid promotes oxidative damage (Delwing-de Lima et al, 2017) and the molecule was found among a panel of metabolites associated with chronic kidney disease (Zhang et al, 2016). It is also noteworthy that Arginine metabolism has been associated earlier to pathogenicity during an investigation of the impact of expression of yersiniabactin-related genes on the primary metabolism of E. coli (Lv and Henderson, 2011), an iron uptake system present in B2 strains subject to our focussed metabolome analysis.…”

Section: Discussionmentioning

confidence: 98%

Substantial Extracellular Metabolic Differences Found Between Phylogenetically Closely Related Probiotic and Pathogenic Strains of Escherichia coli

et al. 2019

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Since its first isolation a century ago, the gut inhabitant Escherichia coli strain Nissle 1917 has been shown to have probiotic activities; however, it is yet not fully elucidated which differential factors play key roles in its beneficial interactions with the host. To date, no metabolomics studies have been reported investigating the potential role of small molecules in functional strain differentiation of Nissle from its genetically close neighbors. Here, we present results of liquid chromatography coupled to high-resolution mass spectrometry characterization of extracellular metabolomes of E. coli strains as a proxy of their bioactivity potential. We found that phylogroup B2 strains exported a more diverse arsenal of metabolites than strains of other phylogroups. Zooming into the phylogroup B2 metabolome identified consistent substantial differences between metabolic output of E. coli Nissle and other strains, particularly in metabolites associated to the Argimine biosynthesis pathway. Nissle was found to release higher levels of Ornithine and Citrulline whilst depleting greater amounts of Arginine from the medium. Moreover, a novel Nissle-specific metabolite not reported before in bacteria, 5-(Carbamoylamino)-2-hydroxypentanoic acid (Citrulline/Arginic Acid related) was observed. Finally, Nissle, CFT073 and NCTC12241/ATCC25922 shared the excretion of N5-Acetylornithine, whereas other strains released N2-Acetylornithine or no N-Acetylornithine at all. Thus, we found substantial metabolic differences in phylogenetically very similar E. coli strains, an observation which suggests that it is justified to further investigate roles of small molecules as potential modulators of the gut environment by probiotic, commensal, and pathogenic strains, including E. coli Nissle 1917.

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“…In embryogenesis, GSH-Px, an enzymatic antioxidant, is highly expressed in most cells and tissues and protects the embryos from oxidative stress (Huo et al ., 2017; Hamdy et al ., 2017; Delwing-de Lima et al ., 2017). In addition to GSH-Px, CAT is another enzymatic antioxidant expressed in embryos and its vital role as an antioxidant has been demonstrated using knock-out and transgenic approaches in mice (Rahman et al ., 2009; Avdesh et al ., 2011).…”

Section: Discussionmentioning

confidence: 99%

The protective effect of melatonin on the in vitro development of yak embryos against hydrogen peroxide-induced oxidative injury

Peng

Lei

Zhang³

et al. 2019

Zygote

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SummaryMelatonin plays a critical role in several types of cells as an antioxidant to protect intracellular molecules from oxidative stress. The anti-oxidation effect of melatonin in yak embryos is largely unknown. We report that melatonin can protect the development of yak preimplantation embryos against oxidative stress induced by hydrogen peroxide (H2O2). Therefore, the quality of blastocysts developed from zygotes exposed to H2O2 was promoted. In addition, we observed that melatonin reduced H2O2-induced intracellular reactive oxygen species (ROS) levels and prevented mitochondrial dysfunction in zygotes. These phenomena revealed the effective antioxidant activity of melatonin to prevent oxidative stress in yak embryos. To determine the underlying mechanism, we further demonstrated that melatonin protected preimplantation embryos from oxidative damage by preserving antioxidative enzymes. Collectively, these results confirmed the anti-oxidation effect of melatonin in yak embryos that significantly improved the quantity and quality of blastocysts in the in vitro production of embryos in yaks.

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Argininic acid alters markers of cellular oxidative damage in vitro : Protective role of antioxidants

Cited by 5 publications

References 41 publications

Lactoferrin ameliorates dopaminergic neurodegeneration and motor deficits in MPTP-treated mice

Lactoferrin ameliorates dopaminergic neurodegeneration and motor deficits in MPTP-treated mice

Substantial Extracellular Metabolic Differences Found Between Phylogenetically Closely Related Probiotic and Pathogenic Strains of Escherichia coli

The protective effect of melatonin on the in vitro development of yak embryos against hydrogen peroxide-induced oxidative injury

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