The protective effect of melatonin on the in vitro development of yak embryos against hydrogen peroxide-induced oxidative injury

Abstract

SummaryMelatonin plays a critical role in several types of cells as an antioxidant to protect intracellular molecules from oxidative stress. The anti-oxidation effect of melatonin in yak embryos is largely unknown. We report that melatonin can protect the development of yak preimplantation embryos against oxidative stress induced by hydrogen peroxide (H2O2). Therefore, the quality of blastocysts developed from zygotes exposed to H2O2 was promoted. In addition, we observed that melatonin reduced H2O2-induced intracellular reactive oxygen species (ROS) levels and prevented mitochondrial dysfunction in zygotes. These phenomena revealed the effective antioxidant activity of melatonin to prevent oxidative stress in yak embryos. To determine the underlying mechanism, we further demonstrated that melatonin protected preimplantation embryos from oxidative damage by preserving antioxidative enzymes. Collectively, these results confirmed the anti-oxidation effect of melatonin in yak embryos that significantly improved the quantity and quality of blastocysts in the in vitro production of embryos in yaks.

Efeito da adição de diferentes antioxidantes em diluentes de criopreservação sobre a qualidade do sêmen e a produção in vitro de embriões bovinos

“…E verificaram que a melatonina reduziu a produção de EROS dos oócitos submetidos ao choque térmico durante a maturação in vitro. Peng et al (2019) também verificaram que a melatonina na concentração de 0 a 100 µM demonstrou efeito positivo na proteção dos oócitos sobre as espécies reativas de oxigênio durante a fertilização in vitro, resultante em melhor qualidade e quantidade de blastocistos produzidos.…”

Simple Colorimetric and Fluorometric Assay Based on 2,3-Naphthalenedialdehyde for Melatonin in Human Saliva

“…In addition, MT has strong neuroendocrine immune regulatory activity, which may become a new antiviral treatment method and approach [9][10][11][12][13]. And it has free radical scavenging antioxidant ability, which slow down the aging of human organs [14][15][16]. Most importantly, MT has a good effect of inhibiting cancer [17][18][19].…”

Characteristics of melatonin-forming disorders of the epiphysis in hemodialysis patients depending on clinical and demographic parameters

“…МТ є гормоном епіфізу, що виконує нейроендокринно-імунну координацію сигналів біологічних процесів в організмі на молекулярному рівні. Доведено, що фізіологічними властивостями МТ є антиоксидантний, імуномоделюючий, антистресорний та антигіпертензивний ефекти [7,8].…”