Arginine Silicate Inositol Complex Accelerates Cutaneous Wound Healing

Abstract

Arginine silicate inositol (ASI) complex is a composition of arginine, silicon, and inositol that has been shown to have beneficial effects on vascular health. This study reports the effects of an ASI ointment on wound healing in rats. A full-thickness excision wound was created by using a disposable 5 mm diameter skin punch biopsy tool. In this placebo-controlled study, the treatment group's wound areas were covered by 4 or 10 % ASI ointments twice a day for 5, 10, or 15 days. The rats were sacrificed either 5, 10, or 15 days after the wounds were created, and biopsy samples were taken for biochemical and histopathological analysis. Granulation tissue appeared significantly faster in the ASI-treated groups than in the control groups (P < 0.05). The mean unhealed wound area was significantly smaller, and the mean percentage of total wound healing was significantly higher in ASI-treated wounds than in the control wounds. Hydroxyproline, collagen, and matrix metalloproteinases were measured in the granulated tissue and found to be affected. Inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), collagen, matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and various cytokines (TNF-α and IL-1β) measured in this study showed a significant fall in expression level in ASI-treated wounds. The results suggest that topical application of ASI ointment (especially 4 % concentration) has beneficial effects on the healing response of an excisional wound.

The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in Rats

“…Jung et al, 2015;Lipner, 2018). In a previous study, we conducted to reveal ASI's properties on wound healing, and we found that granulation tissue grew faster in ASI-treated groups than in the control group (Durmus et al, 2017). Besides, Proctor et al (Proctor et al, 2007) demonstrated that ASI is easily and directly absorbed in vivo, as shown by increased plasma arginine concentrations in ASI-treated rats.…”

“…Photoaging is a skin condition resulting from the chronic and cumulative effects of long-term exposure to UV radiation and is characterized by epidermal thickening, accumulation of abnormal elastin-containing material, deep wrinkles, and abnormal pigmentation (Gragnani et al, 2014). Arginine and biotin exert beneficial effects on vascular function, collagen deposition and synthesis, protein synthesis, and cell proliferation, all of which are crucial for skin health and prevent and control the photoaging process (Dorner et al, 2009;Zempleni et al, 2009;Kalman et al, 2015;Durmus et al, 2017;Mock, 2017). Thus, in the current study, we studied the protective effects of ASI and MgB (a newly produced and highly absorbed biotin salt) orally, with and without MgB topically, on preventing UVB-induced skin photoaging in rats.…”

“…Supplementation with certain nutrients may help prevent and treat skin damage caused by both intrinsic and extrinsic factors. Dietary supplementation with arginine has been shown to facilitate wound healing, enhance insulin sensitivity, collagen deposition, cell proliferation, T-lymphocyte function, protein synthesis, and promote positive nitrogen balance (Dorner et al, 2009;Kalman et al, 2015;Durmus et al, 2017). Silica (Si) is well recognized as effective in preventing skin damage because it plays a significant role in collagen production and connective tissue formation (Seaborn and Nielsen, 2002).…”

An improvement in acute wound healing in rats by the synergistic effect of photobiomodulation and arginine

“…Durmus et al observed a significant increase in expression level of the molecules in ASI-treated skin injuries. Durmus et al concluded that topical use of ASI cream (particularly 4% density) has favorable impacts on the curing reaction of an excisional skin injury simulation in rats [8]. Angiogenesis plays an important function in skin injury repair [10].…”

“…However, abovementioned potentials have not yet advanced to medical use globally [7]. Very recently, Durmus et al proposed that topical use of a mixture of arginine, silicon, and inositol (ASI) cream has favorable impacts on the curing reaction of an excisional skin injury simulation in rats [8]. Further documents for beneficial effectiveness of arginine in skin injury repair in sick persons originates from a probe by Debats et al who described that arginine supplementation hastens superficial skin injuries in sick persons [9].…”