An improvement in acute wound healing in rats by the synergistic effect of photobiomodulation and arginine

Abstract

In this probe, at first we examined the best route and dosage of arginine administration on wound healing in an excisional wound model in rats. Next, we intend to assess the impact of photobiomodulation (PBM) and arginine, individually and together, on the wound healing. In the pilot study, an excisional wound was made in each of 24 rats. There were 4 groups. Group 1 was the control group. In groups 2 and 3, wounds were topically treated with arginine ointments (ARG.) 2% and 5%, respectively. In group 4, arginine was injected (ARG. INJ.,i.p.). In the main phase, in 24 new rats, an excisional wound was made. There were 4 groups: group 5 served as the control. Wounds in group 6 were topically treated with ARG 2%. Wounds in group 7 were subjected to PBM. Wounds in group 8 were treated with PBM+ARG. 2%. On day 15, wound area measurement, wound strength, and stereological examination were performed. In the pilot study, we found that the ARG 2% ointment significantly decreased wound area than ARG. 5%, ARG. INJ. and control groups, and significantly increased wound strength compared to the control and ARG.5% groups. In the main phase, a significant decrease of wound area in all treatment regimens was induced. PBM + ARG. 2% and PBM treatment regimens significantly improved wound strength and almost all stereological parameters, compared to the control and ARG. 2% groups. PBM + ARG. 2% induced anti-inflammatory and angiogenic activities, and hastened the wound healing process in an excisional wound model in rats.

Combined Treatment of Photobiomodulation and Arginine on Chronic Wound Healing in an Animal Model

“…The pulse duration was 180 ns with energy densities of 0.2 J/cm 2 (200 s) and 0.32 J/cm 2 (300 s). 16 Arginine powder (Sigma-Aldrich, USA) (2 g) was dissolved in water, and 98 g of Eucerin was added under sterile conditions to create the ARG ointment. 16 Both PBM and approximately 0.04 g of the ARG ointment were applied to the wounds of groups G4 and G6.…”

“…16 Arginine powder (Sigma-Aldrich, USA) (2 g) was dissolved in water, and 98 g of Eucerin was added under sterile conditions to create the ARG ointment. 16 Both PBM and approximately 0.04 g of the ARG ointment were applied to the wounds of groups G4 and G6. Group G3 received only the ARG ointment, and groups G2 and G5 received only PBM.…”

“…Wound areas were calculated by NIH ImageJ (USA). 16 Wound closure rates were calculated and compared in the study groups and at each time point. The wound closure rate was calculated according to the following formula: (…”

“…Accordingly, we previously reported that a combination of PBM and ARG induced anti-inflammatory and angiogenic activities and hastened the wound healing process in an excisional wound model in rats. 16 The current study assessed the effects of PBM and ARG, individually and together, on wound closure rates, microbial flora, and wound strength in a rat model of ischemic and slow-healing ulcer.…”

Functional Applications of Polyarginine-Hyaluronic Acid-Based Electrostatic Complexes

“…1,2 In case of wound healing, exogenous delivery of Arg was found to accelerate proliferation and remodeling phase of wound healing. 3 Supplemental Arg feeding demonstrated beneficial effects in wound repair. 4,5 Occurrence of an injury is known to activate arginase action around the injury site and an overexpression of the enzyme is likely to impair endogenous Arg supply and diminish iNOS.…”