Arginine functionalized hydroxyapatite nanoparticles and its bioactivity for gene delivery

Wang, Guohui; Zhao, Yanzhong; Tan, Juan; Zhu, Shaihong; Zhou, Kechao

doi:10.1016/s1003-6326(15)63629-9

Cited by 31 publications

(17 citation statements)

References 23 publications

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“…Obviously, there was no essential change in the nanoHAP morphology after being functionalized with AEPH 2 and sulfonic acid. [36] TGA of the nanoparticles was also used to determine the content of organic functional groups of the sample (Fig. 4).…”

Section: Resultsmentioning

confidence: 99%

Direct Synthesis of Nitriles from Aldehydes and Hydroxylamine Hydrochloride Catalyzed by a HAP@AEPH2-SO3H Nanocatalyst

et al. 2017

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We describe an efficient method for the direct preparation of nitriles from aldehydes and hydroxylamine hydrochloride catalyzed by sulfonated nanohydroxyapatite functionalized by 2-aminoethyl dihydrogen phosphate (HAP@AEPH 2 -SO 3 H) as an eco-friendly and recyclable solid acid nanocatalyst. In this protocol the use of a solid acid nanocatalyst provides a green, useful, and rapid method for the preparation of nitriles in excellent yields. In addition, the notable feature of this methodolgy is that the synthesized nanocatalyst can be recovered and reused five times without any noticeable loss of efficiency.

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Section: Resultsmentioning

confidence: 99%

Direct Synthesis of Nitriles from Aldehydes and Hydroxylamine Hydrochloride Catalyzed by a HAP@AEPH2-SO3H Nanocatalyst

et al. 2017

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“…Since the cell membrane is negatively charged, positively charged nanoparticles lead to the higher degree of internalization due to the ionic interactions occurring between positively charged particles and cell membranes [37,38]. Furthermore, it was reported that the positively charged nanoparticles might escape from lysosomes if they are internalized and show perinuclear localization, while the negatively and neutrally charged nanoparticles colocalized with lysosomes [39,40].…”

Section: The Use Of Calcium Phosphate Nanoparticles For Gene Therapymentioning

confidence: 99%

“…Therefore, conjugating the nanoparticles with special functional groups can enhance the cellular uptake and thus the transfection efficiency of the delivery system. Recent research has revealed that arginine with guanidyl group can facilitate the cellular uptake of covalently conjugated particles, although the uptake mechanism is still controversial [37]. Wang et al [37] synthesized rod-like arginine functionalized HA (HA/Arg) nanoparticles with an average length of 50-90 nm and the DNA complex was prepared by mixing the HAp/Arg with plasmid DNA (pEGFP-N1).…”

Section: The Use Of Calcium Phosphate Nanoparticles For Gene Therapymentioning

confidence: 99%

“…Recent research has revealed that arginine with guanidyl group can facilitate the cellular uptake of covalently conjugated particles, although the uptake mechanism is still controversial [37]. Wang et al [37] synthesized rod-like arginine functionalized HA (HA/Arg) nanoparticles with an average length of 50-90 nm and the DNA complex was prepared by mixing the HAp/Arg with plasmid DNA (pEGFP-N1). Transfection efficiency was tested on the HeLa cells which were treated with pEGFP-N1-HA/ Arg complex and the transfection efficiency increased after arginine modification.…”

Section: The Use Of Calcium Phosphate Nanoparticles For Gene Therapymentioning

confidence: 99%

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Gene Delivery by Hydroxyapatite and Calcium Phosphate Nanoparticles: A Review of Novel and Recent Applications

Bakan¹

2018

Hydroxyapatite - Advances in Composite Nanomaterials, Biomedical Applications and Its Technological Facets

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Gene therapy is a targeted therapy which can be used in the treatment of various acquired and inherited diseases. Inhabitation of a gene function, restoring or improving a gene, or gaining a new function can be achieved by gene therapy strategies. The most crucial step in this therapy is delivering the therapeutic material to the target. Nanosized calcium phosphates (CaPs) have been considered as promising carriers due to their excellent biocompatibility. In this chapter, the delivery of DNA, siRNA, and miRNA by using CaP nanocarriers were compiled in detail and the main parameters which can affect the carrier properties and thus the gene transfer efficiency were also discussed.

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“…In recent years, with the development of nanotechnology, many studies have found that hydroxyapatite nanoparticles (HAP) are deemed to be ideal gene carriers because of its good biological stability, easy preparation and modification [1][2][3]. In our previous studies, HAP can transfer a therapeutic gene and a reporter gene in vitro and in vivo [4][5][6]. Simultaneously, arginine-modified hydroxyapatite nanoparticles (HAP/Arg) could enhance gene delivery efficiency [7,8].…”

Section: Introductionmentioning

confidence: 99%

Study on the Biological Safety of Arginine-modified Hydroxyapatite Nanoparticles

Yang¹,

Sun²,

Zhao³

et al. 2016

JAN

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Abstract. This study was to investigate the biological safety of arginine-modified hydroxyapatite nanoparticles (HAP/Arg) as a gene carrier for gene therapy. Experiments were conducted on normal human vascular endothelial cells (HAEC) and human tumor cells (Hela cells) to study the biological safety of HAP/Arg at subcellular level, cellular level, and overall level of animal. Cell proliferation, structural integrity of cell membrane, acute toxicity of HAP/Arg and animal reproductive toxicity of HAP/Arg were all examined. The results showed that HAP/Arg of various doses did not significantly affect normal growth and cell membrane structure of HAEC cells and Hela cells, when these cells were treated for different duration. Acute toxicity experiments and general reproductive toxicity experiments on animals also revealed no significant difference between each HAP/Arg dose group and control group. These results could lay the foundation for the construction of a novel and safe HAP gene delivery system.

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Arginine functionalized hydroxyapatite nanoparticles and its bioactivity for gene delivery

Cited by 31 publications

References 23 publications

Direct Synthesis of Nitriles from Aldehydes and Hydroxylamine Hydrochloride Catalyzed by a HAP@AEPH2-SO3H Nanocatalyst

Direct Synthesis of Nitriles from Aldehydes and Hydroxylamine Hydrochloride Catalyzed by a HAP@AEPH2-SO3H Nanocatalyst

Gene Delivery by Hydroxyapatite and Calcium Phosphate Nanoparticles: A Review of Novel and Recent Applications

Study on the Biological Safety of Arginine-modified Hydroxyapatite Nanoparticles

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