Arginine and Leucine regulate p70 S6 kinase and 4E-BP1 in intestinal epithelial cells

Ban, Hidetoshi; Shigemitsu, Kaori; Yamatsuji, Tomoki; Haisa, Minoru; Nakajo, Tohru; Takaoka, Munenori; Nobuhisa, Tetsuji; Gündüz, Mehmet; Tanaka, Noriaki; Naomoto, Yoshio

doi:10.3892/ijmm.13.4.537

Cited by 92 publications

(93 citation statements)

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“…Numerous other studies have confirmed the role of leucine in activating the mTOR signaling pathway in certain cells including the intestine [37][38][39]. In the presence of leucine or other recently identified amino acids such as arginine, p70 S6 kinase and eIF-4E binding protein 1 are activated by mTOR.…”

Section: Discussionmentioning

confidence: 88%

Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro

Chen¹,

Reimer²

2009

Nutrition

138

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Objective-A growing body of evidence supports an antiobesity effect of dairy products; however, the mechanisms remain unclear. The objective of this study was to explore possible intestinal mechanisms by which dairy delivers an antiobesity effect. The human intestinal cell line, NCI-H716, was used to test the hypothesis that branched-chain amino acids and dairy proteins regulate satiety hormone secretion and modulate genes involved in fatty acid and cholesterol metabolism.Methods-In dose-response (0.5%, 1.0%, 2.0%, and 3.0%) studies, the effect of leucine, isoleucine, valine, skim milk, casein, and whey on glucagon-like peptide-1 release and the expression of selected genes were tested.Results-Leucine, isoleucine, skim milk, and casein stimulated glucagon-like peptide-1 release (P < 0.05). Isoleucine and whey downregulated the expression of intestinal-type fatty acid binding protein (i-FABP), fatty acid transport protein 4 (FATP4), Niemann-Pick C-1-like-1 protein (NPC1L1), acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), sterol regulatory element-binding protein-2 (SREBP-2), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR; P < 0.05). Leucine and valine downregulated the expression of NPC1L1, ACC, FAS, SREBP-2, and HMGCR (P < 0.05). Casein downregulated the expression of i-FABP, FATP4, ACC, FAS, SREBP-2, and HMGCR (P < 0.05). Skim milk downregulated the expression of ACC, FAS, and SREBP-2, but not i-FABP, FATP4, and NPC1L1.Conclusion-This work suggests that the antiobesity effect of dairy may be mediated, at least in part, by integration of events that promote glucagon-like peptide-1 secretion and inhibit expression of genes involved in intestinal fatty acid and cholesterol absorption and synthesis.

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Section: Discussionmentioning

confidence: 88%

Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro

Chen¹,

Reimer²

2009

Nutrition

138

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“…4C and Dataset S28). Arginine, the most proximally enriched metabolite measured (Dataset S29), is required for wound healing (58) and induces proliferation in multiple cells types (59)(60)(61). Glutamine and leucine function with arginine to promote proliferation (62) and were also proximally enriched (Dataset S29).…”

Section: Patterns Of Aldh1l1 and Ca2 In Multiple Fins Of Wild-type Anmentioning

confidence: 99%

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish

Rabinowitz

Robitaille

Wang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Regeneration requires cells to regulate proliferation and patterning according to their spatial position. Positional memory is a property that enables regenerating cells to recall spatial information from the uninjured tissue. Positional memory is hypothesized to rely on gradients of molecules, few of which have been identified. Here, we quantified the global abundance of transcripts, proteins, and metabolites along the proximodistal axis of caudal fins of uninjured and regenerating adult zebrafish. Using this approach, we uncovered complex overlapping expression patterns for hundreds of molecules involved in diverse cellular functions, including development, bioelectric signaling, and amino acid and lipid metabolism. Moreover, 32 genes differentially expressed at the RNA level had concomitant differential expression of the encoded proteins. Thus, the identification of proximodistal differences in levels of RNAs, proteins, and metabolites will facilitate future functional studies of positional memory during appendage regeneration.regeneration | positional memory | growth control | zebrafish | caudal fin

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“…RAPA treatment of patients with SLE normalized cytosolic and mitochondrial Ca 2+ levels and T cell activation-induced rapid Ca 2+ fluxing, without influencing MHP [68], indicating that increased Ca 2+ fluxing is downstream or independent of MHP in the pathogenesis of T-cell dysfunction in SLE. Interestingly, the mammalian target of rapamycin (mTOR) may be activated by enhanced production of NO [69]. The effectiveness of RAPA in murine and human SLE suggest that mTOR is a potential sensor [70] and down-stream effector of MHP and activation of mTOR may precede T cell dysfunction and autoimmunity in SLE.…”

Section: Mitochondrial Hyperpolarization and Increased No Production mentioning

confidence: 99%

Nitric oxide, mitochondrial hyperpolarization, and T cell activation☆

Nagy

Koncz

Fernández

et al. 2007

Free Radical Biology and Medicine

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T lymphocyte activation is associated with nitric oxide (NO) production that plays an essential role in multiple T cell functions. NO acts as a messenger, activating soluble guanyl cyclase and participating in the transduction signaling pathways involving cyclic GMP. NO modulates mitochondrial events that are involved in apoptosis and regulates mitochondrial membrane potential and mitochondrial biogenesis in many cell types, including lymphocytes. Mitochondrial hyperpolarization (MHP), an early and reversible event during both T lymphocyte activation and apoptosis, is regulated by NO. Here, we discuss recent evidence that NO-induced MHP represents a molecular switch in multiple T cell signaling pathways. Overproduction of NO in systemic lupus erythematosus (SLE) induces mitochondrial biogenesis and alters Ca 2+ signaling. Thus, while NO plays a physiological role in lymphocyte cell signaling, its overproduction may disturb normal T cell function, contributing to the pathogenesis of autoimmunity.

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Arginine and Leucine regulate p70 S6 kinase and 4E-BP1 in intestinal epithelial cells

Cited by 92 publications

References 0 publications

Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro

Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish

Nitric oxide, mitochondrial hyperpolarization, and T cell activation☆

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