Argentation chromatography on silver sulfamate‐impregnated silica gel layers

Ilinov, P

doi:10.1007/bf02533540

Cited by 13 publications

(2 citation statements)

References 13 publications

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“…Although the influence of the salt anion has not been studied systematically, there is evidence that its nature may affect the sample resolution. For example, silver sulphamate [24] and silver benzenesulphonate [25] have been used in TLC adsorbents and were reported to give improved separations of FAME. On the other hand, some exchange of anions with the calcium salt used as binder is inevitable, so the effect of the anion can not be a simple one.…”

Section: Silver Ion Tlcmentioning

confidence: 99%

Stationary phases for silver ion chromatography of lipids: Preparation and properties

Momchilova

Nikolova‐Damyanova²

2003

J of Separation Science

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Procedures for the preparation of stationary phases for several silver ion chromatographic techniques have been surveyed. Thin-layer chromatography (TLC), conventional (normal pressure) column chromatography, solid phase extraction (SPE), highperformance liquid chromatography (HPLC), and supercritical fluid chromatography (SFC) in silver ion mode have been considered. The utilization of these stationary phases for the analysis of fatty acids and triacylglycerols has been demonstrated.

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Section: Silver Ion Tlcmentioning

confidence: 99%

Stationary phases for silver ion chromatography of lipids: Preparation and properties

Momchilova

Nikolova‐Damyanova²

2003

J of Separation Science

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“…Investigation of lipid composition in plant tissues frequently involves preparation of fatty acid methyl esters (FAME) derived from glycerolipids. FAME analysis and separation typically are achieved by a variety of chromatography methods, such as gas chromatography (GC), high-performance liquid chromatography (HPLC), and thin-layer chromatography (TLC) (1)(2)(3)(4)(5)(6)(7). The method of choice depends on the objectives of the investigation and available resources.…”

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confidence: 99%

An improved reversed‐phase thin‐layer chromatography method for separation of fatty acid methyl esters

Marquardt

Wilson

1998

J Americ Oil Chem Soc

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Resolution of fatty acid methyl esters (FAME) by thin-layer chromatography often is complicated by co-migration of certain acyl-isomers in heterogeneous mixtures. However, a novel reversed-phase thin-layer chromatography method which employs 10% (wt/vol) silver nitrate in a mobile phase containing acetonitrile/1,4-dioxane/acetic acid (80:20:1, vol/vol/vol) allows one-dimensional resolution of a wide range of acyl-methyl esters. This innovation enables improved separation of saturated FAME ranging from C 12 to C 22 , and geometric isomers of C 14 to C 22 unsaturated FAME by thin-layer chromatography.Investigation of lipid composition in plant tissues frequently involves preparation of fatty acid methyl esters (FAME) derived from glycerolipids. FAME analysis and separation typically are achieved by a variety of chromatography methods, such as gas chromatography (GC), high-performance liquid chromatography (HPLC), and thin-layer chromatography (TLC) (1-7). The method of choice depends on the objectives of the investigation and available resources. When TLC is practical, a two-dimensional approach typically is required to obtain acceptable resolution of certain FAME. As an example, silica gel impregnated with silver nitrate (AgNO 3 ) in a normal phase system may be used to separate FAME by degree of unsaturation. In this system, saturated FAME advance farthest, then monounsaturated, and finally polyunsaturated acyl methyl esters. Resolution of saturated FAME may then be enhanced by employing C 18 reversed-phase gels (RPTLC) in a second dimension where separation depends on carbon number. On RPTLC, FAME interact with a polar solvent on a nonpolar stationary phase in a manner that allows more rapid migration of molecules with greater polarity and lower mass. Both techniques usually are necessary because different FAME containing the same degree of unsaturation are not resolved well by AgNO 3 -TLC, and FAME like 16:0 and ∆9c-18:1 tend to co-migrate in RPTLC systems. Thus, AgNO 3 -TLC may be used to separate FAME by number of unsaturated bonds in one dimension, and reversed-phase TLC may be used to separate FAME by carbon number in a second dimension. FAME may then be visualized by a number of detection methods (4,5) and subsequently quantified by GC or scraped into scintillation vials if the sample is radiolabeled.Given the desirable attributes of each method in the traditional two-dimensional approach, an attempt was made to improve the logistical efficiency of FAME resolution by TLC. A one-dimensional TLC system was developed using silver ions within the mobile phase and a reversed-phase C 18 (n-octadecylsilyl) silica stationary phase. Although addition of silver ions in the mobile phase of reversed-phase TLC has been reported (8), this innovation enabled an improved single-dimensional TLC method with the capability of resolving a wide range of FAME standards, including geometric isomers of unsaturated acyl methyl esters. MATERIALS AND METHODSTwenty-four FAME standards with greater than 99% purity were purchased from ...

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Direct resolution of propranolol and bupranolol by thin-layer chromatography using cellulose derivatives as stationary phase

Suedee

Heard

1997

Chirality

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Argentation chromatography on silver sulfamate‐impregnated silica gel layers

Cited by 13 publications

References 13 publications

Stationary phases for silver ion chromatography of lipids: Preparation and properties

Stationary phases for silver ion chromatography of lipids: Preparation and properties

An improved reversed‐phase thin‐layer chromatography method for separation of fatty acid methyl esters

Direct resolution of propranolol and bupranolol by thin-layer chromatography using cellulose derivatives as stationary phase

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