ARG3.1/ARC expression in hippocampal dentate gyrus astrocytes: ultrastructural evidence and co‐localization with glial fibrillary acidic protein

Rodrı́guez, José J.; Davies, Heather A.; Er̀rington, M.L.; Verkhratsky, Alexei; Bliss, T. V. P.; Stewart, M. G.

doi:10.1111/j.1582-4934.2007.00105.x

Cited by 22 publications

(17 citation statements)

References 39 publications

(76 reference statements)

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“…Similar to our results, Arc was previously found to be neuron specific [ 32 ]; in the cellular soma and nucleus [ 32 – 34 ]. However, contrary to our findings, Rodriguez reported Arc presence in glia cells [ 35 ].…”

Section: Discussioncontrasting

confidence: 99%

Time Course of Immediate Early Gene Protein Expression in the Spinal Cord following Conditioning Stimulation of the Sciatic Nerve in Rats

et al. 2015

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Long-term potentiation induced by conditioning electrical stimulation of afferent fibers is a widely studied form of synaptic plasticity in the brain and the spinal cord. In the spinal cord dorsal horn, long-term potentiation is induced by a series of high-frequency trains applied to primary afferent fibers. Conditioning stimulation (CS) of sciatic nerve primary afferent fibers also induces expression of immediate early gene proteins in the lumbar spinal cord. However, the time course of immediate early gene expression and the rostral-caudal distribution of expression in the spinal cord have not been systematically studied. Here, we examined the effects of sciatic nerve conditioning stimulation (10 stimulus trains, 0.5 ms stimuli, 7.2 mA, 100 Hz, train duration 2 s, 8 s intervals between trains) on cellular expression of immediate early genes, Arc, c-Fos and Zif268, in anesthetized rats. Immunohistochemical analysis was performed on sagittal sections obtained from Th13- L5 segments of the spinal cord at 1, 2, 3, 6 and 12 h post-CS. Strikingly, all immediate early genes exhibited a monophasic increase in expression with peak increases detected in dorsal horn neurons at 2 hours post-CS. Regional analysis showed peak increases at the location between the L3 and L4 spinal segments. Both Arc, c-Fos and Zif268 remained significantly elevated at 2 hours, followed by a sharp decrease in immediate early gene expression between 2 and 3 hours post-CS. Colocalization analysis performed at 2 hours post-CS showed that all c-Fos and Zif268 neurons were positive for Arc, while 30% and 43% of Arc positive neurons were positive for c-Fos and Zif268, respectively. The present study identifies the spinal cord level and time course of immediate early gene (IEGP) expression of relevance for analysis of IEGPs function in neuronal plasticity and nociception.

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Section: Discussioncontrasting

confidence: 99%

Time Course of Immediate Early Gene Protein Expression in the Spinal Cord following Conditioning Stimulation of the Sciatic Nerve in Rats

et al. 2015

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“…NMDA receptor activation is a critical step both for synaptic plasticity and Arc activation [43]. Although Arc activation in the GCL is considered neuronal specific [44], presence of Arc has been reported in reactive astrocytes in the molecular layer of the DG [45]. Counts related to Arc-induction in this study, however, were restricted to the GCL layer.…”

Section: Discussionmentioning

confidence: 78%

Functional integration of new neurons into hippocampal networks and poststroke comorbidities following neonatal stroke in mice

Kadam

Smith‐Hicks

Smith

et al. 2010

Epilepsy & Behavior

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Stroke in the developing brain is an important cause of chronic neurological morbidities including neurobehavioral dysfunction and epilepsy. Here, we describe a mouse model of neonatal stroke resulting from unilateral carotid ligation that results in acute seizures, long-term hyperactivity, spontaneous lateralized circling behavior, impaired cognitive function and epilepsy. Explorationdependent induction of immediate early gene, Arc (activity-regulated cytoskeleton associated protein) in hippocampal neurons was examined in the general population of neurons versus neurons that were generated ~ 1week after the ischemic insult and labeled with BrdU. Although Arc was inducible in a network specific manner after severe neonatal stroke, it was impaired, not only in the ipsilateral injured but also in the contralateral uninjured hippocampi when examined at 6 months after the neonatal stroke. Severity of the stroke-injury and the acquired post-stroke epilepsy, both negatively correlated with Arc-induction and new neuron integration into functional circuits in the injured hippocampi.

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“…Interestingly, a few puncta were found located outside the MAP2 positive cells. These may reflect a low level of background stain or the synthesis of Arc within astorcytes (Rodriguez et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

Secreted Amyloid Precursor Protein-Alpha Promotes Arc Protein Synthesis in Hippocampal Neurons

Livingstone

Elder

Barrett

et al. 2019

Front. Mol. Neurosci.

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Secreted amyloid precursor protein-α (sAPPα) is a neuroprotective and memory-enhancing molecule, however, the mechanisms through which sAPPα promotes these effects are not well understood. Recently, we have shown that sAPPα enhances cell-surface expression of glutamate receptors. Activity-related cytoskeletal-associated protein Arc (Arg3.1) is an immediate early gene capable of modulating long-term potentiation, long-term depression and homeostatic plasticity through regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor localization. Accordingly, we hypothesized that sAPPα may enhance synaptic plasticity, in part, by the de novo synthesis of Arc. Using primary cortical and hippocampal neuronal cultures we found that sAPPα (1 nM, 2 h) enhances levels of Arc mRNA and protein. Arc protein levels were increased in both the neuronal somata and dendrites in a Ca 2+ /calmodulin-dependent protein kinase II-dependent manner. Additionally, dendritic Arc expression was dependent upon activation of mitogen-activated protein kinase and protein kinase G. The enhancement of dendritic Arc protein was significantly reduced by antagonism of N -methyl- D -aspartate (NMDA) and nicotinic acetylcholine (α7nACh) receptors, and fully eliminated by dual application of these antagonists. This effect was further corroborated in area CA1 of acute hippocampal slices. These data suggest sAPPα-regulated plasticity within hippocampal neurons is mediated by cooperation of NMDA and α7nACh receptors to engage a cascade of signal transduction molecules to enhance the transcription and translation of Arc.

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ARG3.1/ARC expression in hippocampal dentate gyrus astrocytes: ultrastructural evidence and co‐localization with glial fibrillary acidic protein

Cited by 22 publications

References 39 publications

Time Course of Immediate Early Gene Protein Expression in the Spinal Cord following Conditioning Stimulation of the Sciatic Nerve in Rats

Time Course of Immediate Early Gene Protein Expression in the Spinal Cord following Conditioning Stimulation of the Sciatic Nerve in Rats

Functional integration of new neurons into hippocampal networks and poststroke comorbidities following neonatal stroke in mice

Secreted Amyloid Precursor Protein-Alpha Promotes Arc Protein Synthesis in Hippocampal Neurons

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