Are Tγ cells of myelomonocytic lineage?

Astaldi, A; Griend, R J van de; Schellekens, P. T. A.; Vossen, Jaak M.

doi:10.1002/eji.1830120615

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…The latter also implies that the OKM-1 determinant is not exclusively expressed on non-T cells as a small portion of the OKM-T cells carry the T3 marker. Following different avenues, a similar conclusion has been reached by Astaldi et al (1982). The data presented so far strongly suggests that T8* cells of the suppressor lineage bearing FcyR (T8*y*) can be classified as T suppressor effector cells.…”

Section: Suppressor Effector T Cells Present In Vivosupporting

confidence: 68%

Immunoregulatory T Cell Subpopulations in Man: Dissection by Monoclonal Antibodies and Fc‐Receptors

Ballieux¹,

Heijnen

1983

Immunological Reviews

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Until now, most of the studies on regulatory T cells have been based on culture systems in which human peripheral blood cells are stimulated by polyclonal stimulators like Pokeweed Mitogen (PWM). Our present contribution, however, deals with T cell-mediated regulation of the antigen-induced B cell activation, which exclusively leads to an antigen-specific IgM production (Heijnen et al. 1979a). Some authors' reports on regulatory activities of T cells, as tested in systems using polyclonal stimulators, differ from ours. This may be due to: a) as a result of polyclonal stimulus, various types of regulatory T cells are activated at the same time b) in contrast to a primary antigen, a polyclonal stimulator induces a rapid proliferation of the various regulatory T cells c) a polyclonal stimulator induces the differentiation of B cells in various maturational stages, that might each require additional or different regulatory signals. For example, Thomas et al. (1981) have shown that freshly isolated T4+ cells can induce suppressor activity in unprimed T8+ cells in the presence of PWM, whereas T4+ cells, precultured for 24 h in the presence of PWM, can exert a suppressor activity themselves without an apparent need for T8+ cells. In the antigen-specific system, however, we have neither been able to detect T suppressor effector activity in a population of primed T4+ cells, nor been able to demonstrate T suppressor inducer activity in unprimed T4+ cells (Heijnen et al. 1982a). Therefore the state of activation of the total T4+ population will dictate the balance of the total T helper and T suppressor activity. As a result of proliferation induced by polyclonal mitogens, small subsets of regulatory T cells, which are functionally undetectable in the primary antigen-specific assay, can expand sufficiently to have a measurable effect. Thomas et al. (1980) have shown that the T4+ suppressor inducer cell in the PWM system is radio-sensitive, which is in contrast with our data in the antigen-specific system. This may imply that we are looking at different subsets of T suppressor inducer cells in these different systems, but it might also indicate that T suppressor inducer cells need to proliferate in order to be able to measurably regulate the large pool of responding cells generated in the PWM system. Apart from such quantitative effects, polyclonal B cell activators like PWM are capable of inducing the differentiation of B cells in various maturational stages (Kuritani & Cooper 1982, Stevens 1982, Peters & Fauci 1983). Since it is highly likely that the regulation of these various B cell subsets might require different regulatory signals, the PWM model might be a very complicated model to study regulatory effects of single T cell subsets.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Suppressor Effector T Cells Present In Vivosupporting

confidence: 68%

Immunoregulatory T Cell Subpopulations in Man: Dissection by Monoclonal Antibodies and Fc‐Receptors

Ballieux¹,

Heijnen

1983

Immunological Reviews

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“…This phenomenon, however, does not imply that the Ty+ fraction contains only 30% T cells. Astaldi et al [26] and Van der Griend et al [27] clearly showed that OKT3 does not identify all T cells. Thus, most of the T3-y' cells rosetting with SE belong (at least for the greater part) to the T cell lineage.…”

Section: Definition Of T-inducer Cell Activitymentioning

confidence: 99%

Characterization of human T suppressor‐inducer, ‐precursor and ‐effector lymphocytes in the antigen‐specific plaque‐forming cell response

Heijnen

Pot

Ballieux³

1982

Eur J Immunol

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Suppression of an antigen-specific plaque-forming cell response of human blood lymphocytes can be effected by T mu+ cells that have been primed previously by antigen in vitro for 6 days. While lacking the capacity to suppress the plaque-forming response directly, these primed T mu+ suppressor-inducer cells stimulate a subpopulation of unprimed T mu gamma- cells to differentiate to T gamma + suppressor-effector cells. The T mu+, T gamma+ and T mu gamma- subsets have been shown to be heterogeneous populations of cells. Therefore, the functionally defined T suppressor-inducer, -precursor and -effector cells were characterized by OKT monoclonal antibodies and by the capacity to form rosettes with autologous erythrocytes (ar+). Evidence will be presented that in vitro a T4+mu+ar- cell induces a T8+mu gamma-ar+ precursor cell to differentiate to a T8+gamma+ar- suppressor-effector cell. A similar T suppressor-effector cell can also be isolated directly from peripheral blood of normal donors.

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Characterization of human granular lymphocyte subpopulations expressing HNK‐1 (Leu‐7) and Leu‐11 antigens in the blood and lymphoid tissues from fetuses, neonates and adults

1984

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Three subpopulations of human granular lymphocytes from blood and lymphoid tissues were characterized using combinations of the monoclonal antibodies (mAb) HNK-1 (Leu-7), Leu-11 and VEP13. Each subpopulation was confirmed to possess natural killer (NK) cell functional capability, but a different level of cytotoxic efficiency (HNK-1+leu-11- less than HNK-1+Leu-11+ less than HNK-1-Leu-11+). In adult from 23 healthy donors, the subpopulations with HNK-1+Leu-11-, HNK-1+Leu-11+ and HNK-1-Leu-11+ phenotypes comprised 4.7 +/- 3.0, 8.0 +/- 6.4 and 3.9 +/- 3.5% of mononuclear cells, respectively. Despite their distinct surface marker phenotypes and NK functional ability, all 3 subpopulations exhibited granular lymphocyte morphology. One of these subpopulations, HNK-1+Leu-11-, also expressed the pan-T cell antigen Leu-4. Different patterns were observed in fetal bone marrow and cord blood, where the vast majority of HNK-1+ cells lacked the Leu-11 antigen (HNK-1+Leu-11+ cells). The HNK-1 antigen was not expressed on granulocytes and their precursors, whereas both Leu-11 and VEP13 antigens were expressed on these myeloid cells from fetal bone marrow and cord blood as well as adult bone marrow and spleen. Cell lines of granular lymphocytes cultured in the presence of interleukin 2 all possessed the HNK-1+Leu-4+ phenotype and NK functional capability but lacked the Leu-11 and VEP13 antigens on their surface after 15 days of culture. Although granular lymphocytes expressing the Fc receptors reacting with the mAb Leu-11 and VEP13, are the most functionally active NK cells, the HNK-1+ subpopulation lacking the Leu-11 and VEP13 antigens appears to be an important population (possibly an immature form of granular lymphocytes) for delineating the cell lineage(s) and differentiation of human granular lymphocytes. Although none of the currently available mAb react both inclusively and exclusively with human granular lymphocytes, the combination usage of these antibodies permits a more precise and comprehensive analysis of these subsets.

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Are Tγ cells of myelomonocytic lineage?

Cited by 4 publications

References 18 publications

Immunoregulatory T Cell Subpopulations in Man: Dissection by Monoclonal Antibodies and Fc‐Receptors

Immunoregulatory T Cell Subpopulations in Man: Dissection by Monoclonal Antibodies and Fc‐Receptors

Characterization of human T suppressor‐inducer, ‐precursor and ‐effector lymphocytes in the antigen‐specific plaque‐forming cell response

Characterization of human granular lymphocyte subpopulations expressing HNK‐1 (Leu‐7) and Leu‐11 antigens in the blood and lymphoid tissues from fetuses, neonates and adults

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