Are C-KIT, MMP-9 and Type II Collagen Positive Undifferentiated Cells Involved in Cartilage Growth? A Description of Unusual Interstitial Type of Cartilage Growth

Soliman, Soha A.; Abd‐Elhafeez, Hanan H.

doi:10.4172/2157-7099.1000440

Cited by 16 publications

(20 citation statements)

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“…We applied digital coloring for the transmission electron microscopic images using the Photo Filter 6.3.2 program to identify different types of cells and structures. Many authors previously used the methods 12,[63][64][65][66][67] . CMEIAS color segmentation (for supplementary images).…”

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confidence: 99%

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Abd‐Elhafeez

Abou-Elhamd

Soliman

2020

Sci Rep

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the current study investigated telocytes (tcs) in the intestinal bulb of Grass carp using light microscopy (LM), transmission electron microscopy (teM), scanning electron microscopy, and immunohistochemistry (iHc). By LM, tcs were distinguished by the typical morphological features that had a cell body and telopodes using He, toluidine blue, methylene blue, Marsland silver stain, Grimelius's silver nitrate, Giemsa, PAS, combined AB pH2,5/PAS, Crossmon's and Mallory triple trichrome, Van Gieson stains, Verhoeff's stain, Sudan black, osmic acid, performic acid with methylene blue and bromophenol blue. TCs were identified under the epithelium as an individual cell or formed a TCs sheath. They detected in the lamina propria, between muscle fibers, around the myenteric plexus and fibrous tissue. TCs acquired immunological features of endocrine cells that exhibited high affinity for silver stain, performic acid with methylene blue, Marsland stain, and immunohistochemical staining using chromogranin A. Sub epithelial tcs were closely related to the endocrine cells. tcs and their secretory activities were recognized using acridine orange. TCs were identified by IHC using CD34, CD117, S100-protein, desmin. TCs formed a3D network that established contact with macrophage, mast cells, dendritic cells, lymphocytes, smooth muscle fibers, fibroblast, Schwann cells and nerve fibers. In conclusion, the localization of TCs in relation to different types of immune cells indicated their potential role in the maintenance of intestinal immunity. Interstitial cells have critical roles in the maintenance of the appropriate 3D scaffold and functional requirements of the organs. Telocytes (TCs) are the cell population that forms a network through a labyrinthine system formed by telopodes. They are long and slender prolongations reached up to hundreds of microns. Telopodes are identified by their segments; the thin segment, podomer and the thick segment, the podoms. TCs established contact to other resident cells or to wandering cells via their telopodes or via the cell body. Telopodes network provide-long-distance cell-cell signaling (intercellular communication) 1. Paracrine signaling is critical for TCS function. TCs produce several secretory molecules and factors contributing to the functional significance.Gene analysis 2 and proteomics analysis 3 are investigated for TCs. The functional contributions of TCS are supposed to be related to angiogenesis 4 , and development, maintenance of homeostatic balance 4 , immunosurveillance 5 , tissue regeneration and repair through providing adequate microenvironment for stem cell niche and promoting their differentiation 6,7. TCs are identified in a wide variety of organs from diverse species including mammals 8-11 , avian 12,13 , reptiles 14 , Amphibians 15 and aquatic species 16,17 and parasitic worm 18. They are located in the trachea and lungs 19 , heart 20 and the blood vessels 21 , kidney, ureter, urinary bladder 22 , tongue 23 , oesophagus, stomach and small and large

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confidence: 99%

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Abd‐Elhafeez

Abou-Elhamd

Soliman

2020

Sci Rep

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“…Sample processing for embedding in paraffin was performed according to Soliman and Abd‐Elhafeez (). The protocols for histochemical staining using H&E, safranin O, methylene blue, Mallory trichrome, and PAS were performed following Abd‐Elhafeez and Soliman (), and orange G staining was performed according to Aksoy et al () and Krishna and Kumar ().…”

Section: Methodsmentioning

confidence: 99%

Morphological and Histochemical Description of Quail Feather Development

Soliman

2019

The Anatomical Record

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The current study investigated the macroscopic and microscopic differences between pennaceous and plumulaceous feathers. The morphology of the barbules distinguished pennaceous and plumulaceous feathers, particularly the shape of barbules during their development. In pennaceous feathers, the initial barbules were large and elongated or pyriform in shape, while plumulaceous feathers had small, thin, elongated initial barbules. The spinous barbules were characteristic of pennaceous feathers. The histochemical reactivity of both feather types for Mallory trichrome, orange G, and acridine orange, safranin O, PAS, and methylene blue was determined. Keratin was detected by Mallory trichrome, orange G, and acridine orange. In conclusion, the histochemical properties of pennaceous and plumulaceous feathers of quail, particularly the distribution and nature of keratin during development, should be considered in future studies. The unique morphological features of pennaceous and plumulaceous feathers could be used as a guide for phylogenetic identification. Anat Rec, 2019. © 2019 American Association for Anatomy Anat Rec, 303:1865–1883, 2020. © 2019 American Association for Anatomy

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“…Several articles (Abd-Elhafeez & Soliman, 2016, 2017; Soliman & Abd-Elhafeez, 2016 a , 2016 b ; Soha Soliman et al, 2017, Soliman & Emeish, 2017; Soliman & Madkour, 2017; Fatma El-Zahraa & Abd-Elhafez, 2018; Soliman, 2019 b ) have used the same methods for coloring of transmission electron micrographs.…”

Section: Methodsmentioning

confidence: 99%

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

et al. 2020

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Studying the dermal skeleton in fish is valuable for phylogenetic specification. The current study describes the detailed structure of the plecostomus dermal skeleton, including its morphogenesis and distribution in the skin. The denticles have a crown and a basal part and are embedded in bony depressions, to which they are attached by denticle ligaments. During denticle morphogenesis, denticle papillae formed from denticle precursor cells align in two cellular layers: an outer ameloblast precursor layer and an inner odontoblast precursor layer. The ameloblast precursors and odontoblast precursors differentiate and secrete enamel and dentine, respectively. We used different histochemical techniques, including Crossmon's trichrome staining, Weigert–Van Gieson staining, periodic acid–Schiff (PAS) staining, combined Alcian blue (AB; pH 2.5)/PAS staining, Weigert–Van Gieson staining, Mallory trichrome staining, and AB staining to distinguish the dentine and denticle ligaments. We used acridine orange to detect lysosome activity during denticle eruption. Transmission electron microscopy was used to detect the denticle ultrastructure, and scanning electron microscopy was used to detect the topographic distributions of different types of dermal tissues in different anatomical regions.

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Are C-KIT, MMP-9 and Type II Collagen Positive Undifferentiated Cells Involved in Cartilage Growth? A Description of Unusual Interstitial Type of Cartilage Growth

Cited by 16 publications

References 0 publications

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Morphological and Histochemical Description of Quail Feather Development

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

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