Arcobacter species detection in Italian composite foods

Mottola, Anna; Marchetti, Patrizia; Ciccarese, Giuseppina; Terio, Valentina; Sinisi, Carla; Pinto, Angela Di

doi:10.1016/j.lwt.2020.110161

Cited by 4 publications

(2 citation statements)

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“…The isolation of Arcobacter spp. from vegetables was performed in accordance with González and Ferrús ( 2011 ) and with Mottola et al ( 2016b , 2020 ). Briefly, 20 g of sample were added to 180 mL (1:10 wt/vol) of Arcobacter Broth (AB) (Oxoid, Milan, Italy), in sterile bags, and homogenized using a stomacher (PBI International, Milan, Italy) at 11 000 rev min -1 for 2 min.…”

Section: Methodsmentioning

confidence: 99%

“…Given the limited information about the occurrence of arcobacters in vegetables and the wide use of vegetables in the preparation of composite foods (Mottola et al ., 2020 ), the aim of the present study was to assess the occurrence, the presence of virulenceassociated genes and genotyping isolates of Arcobacter spp. isolated from RTE vegetables, in order to elucidate and achieve a specific risk assessment for the sector.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and characterization of <em>Arcobacter</em> spp. from ready-to-eat vegetables produced in Southern Italy

et al. 2021

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Given that the number of foodborne illness outbreaks linked to the consumption of ready-to-eat vegetables has been widely documented and considering that data on the occurrence of Arcobacter spp. in such foodstuffs are lacking, the aim of the present study was to evaluate the presence of Arcobacter spp. and the occurrence of virulence factors as well as to genotype Arcobacter spp. in ready-to-eat (RTE) vegetable samples, using cultural and biomolecular assays. Arcobacter spp. was detected in 16/110 (14.5%) samples, with A. butzleri being detected in 15/16 and A. cryaerophilus in 1/16 isolates. PCRs aimed at the nine putative virulence genes demonstrated widespread distribution of such genes among A. butzleri and A. cryaerophilus isolates. In addition, multilocus sequence type (MLST) analysis revealed a low genetic diversity within the arcobacters isolates. The results underline the need to develop an appropriate surveillance system based on biomolecular characterization for an integrated microbiological risk assessment of ready-toeat vegetables, and consequently of composite foods.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%