An experiment was conducted to determine e¡ects of feeding levels on growth performance, feed utilization, nutrient deposition, body composition and apparent digestibility coe⁄cients (ADCs) of nutrients for juvenile Chinese sucker (initial weight, 11.77 AE 0.22 g). Chinese sucker were fed a practical diet from 0% (starvation) to 4.0% (at 0.5% increments) body weight (bw) day À 1 for 8 weeks. The results showed that growth performance, feed utilization, nutrient deposition, body composition and ADCs of dry matter, protein and energy were signi¢cantly (Po0.05) a¡ected by feeding levels. Survival was the lowest for the starvation group. Final mean body weight, growth rate, thermal-unit growth coe⁄cient (TGC) increased with feeding rate from 0% to 3.0% bw day À 1 (Po0.05) and showed no signi¢cant di¡erences above the level (P40.05). Feed conversion rate was signi¢cantly lower at a feeding level of 2.5% bw day À 1 than above and below the level (Po0.05). Protein e⁄ciency ratio was markedly highest at the 2.5% bw day À 1 ration level (Po0.05). Fish fed at the feeding level (1.0% bw day À 1 ), which represented a maintenance ration (energy gain was less than 2.27 kJ ¢sh À 1 day À 1 ), showed positive protein deposition but negative lipid deposition. This indicates that ¢sh fed a maintenance ration mobilize body lipid reserve to support protein deposition. Lipid contents of whole body, muscle and liver increased with increasing feeding rates from 0.5% to 3.0% bw day À 1 and showed no signi¢cant di¡erences above the level (P40.05). Protein contents of wholebody composition increased with feeding rate from 0.5 to 3.0% bw day À 1 (Po0.05) and showed no sig-ni¢cant di¡erences above the level (P40.05), whereas muscle and liver remained relatively stable with the di¡erent ration amount with the exception of ¢sh fed a ration of 0.5% bw day À 1 , at which Chinese sucker possessed signi¢cantly lower body protein concentration (Po0.05). The ADCs of dry matter, protein and energy decreased with increasing feeding levels from 0.5% to 3.0% bw day À 1 and then remained relatively constant over the level. Based on the broken-line regression analysis using WG data, the optimum and maintenance feeding levels for Chinese sucker were 3.10% bw day À 1 and 0.45% bw day À 1 respectively. imported from USA.wSoybean meal, crude protein 46.4% dry matter, crude lipid 1.9% dry matter; obtained from Gao Long Diary. zGao Long Diary. ‰Vitamin premix (mg kg À 1 diet): thiamine 50 mg; ribo£avin, 90 mg; pyridoxine HCl, 40 mg; vitamin B 12 , 0.2 mg; vitamin K 3 , 20 mg; inositol, 1600 mg; pantothenic acid, 120 mg; niacin acid, 400 mg; folic acid, 40 mg; biotin, 2.40 mg; retinol acetate, 64 mg; cholecalciferol, 10 mg; a-tocopherol, 240 mg; ascorbic acid, 4000 mg; choline chloride, 5000 mg; ethoxyquin 300 mg, wheat middling, 36.78 g. zMineral premix (mg or g kg À 1 diet): NaF, 2 mg; KI, 0.8 mg; CoCl 2 Á 6H 2 O (1%), 50mg; CuSO 4 Á 5H 2 O, 10 mg; FeSO 4 Á H 2 O, 80 mg; ZnSO 4 Á H 2 O, 50 mg; MnSO 4 Á H 2 O,60 mg; MgSO 4 Á 7H 2 O, 1200 mg; Ca(H 2 PO 3 ) 2 Á ...