Architecting of an aptasensor for the staphylococcus aureus analysis by modification of the screen-printed carbon electrode with aptamer/Ag–Cs-Gr QDs/NTiO2

Ghalkhani, Masoumeh; Sohouli, Esmail; Khaloo, Shokooh Sadat; Vaziri, Mohammad Hossein

doi:10.1016/j.chemosphere.2022.133597

Cited by 37 publications

(7 citation statements)

References 58 publications

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“…04–0784), Av–Au NCs has no acicular diffraction peaks of gold-based nanomaterials but only two wide peaks at 17° and 61°. It might be due to the small size [ 42 ], and less content [ 43 ] of Av–Au NCs, which implies that the Au atoms mainly exist in the form of Au clusters [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Fabrication of avidin-stabilized gold nanoclusters with dual emissions and their application in biosensing

Tang

Chen²,

Wang

et al. 2022

J Nanobiotechnol

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Protein-stabilized gold nanoclusters (Prot-Au NCs) have been widely used in biosensing and cell imaging owing to their excellent optical properties and low biotoxicity. However, several Prot-Au NCs reported in the literature do not retain the biological role of the protein, which greatly limits their ability to directly detect biomarkers. This study demonstrated for the first time the successful synthesis of dual-function avidin-stabilized gold nanoclusters (Av–Au NCs) using a one-pot method. The resulting Av–Au NCs exhibited intense blue and red emissions under 374 nm excitation. Furthermore, the Av–Au NCs retained the native functionality of avidin to bind to biotin. When DNA strands modified with biotin at both ends (i.e., linker chains) were mixed with Av–Au NCs, large polymers were formed, indicating that Av–Au NCs could achieve fluorescence signal amplification by interacting with biotin. Taking advantage of the aforementioned properties, we constructed a novel enzyme-free fluorescent biosensor based on the Av–Au NCs-biotin system to detect DNA. The designed fluorescent biosensor could detect target DNA down to 0.043 nM, with a wide line range from 0.2 nM to 20 µM. Thus, these dual-functional Av–Au NCs were shown to be an excellent fluorescent material for biosensing. Graphic abstract

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Section: Resultsmentioning

confidence: 99%

Fabrication of avidin-stabilized gold nanoclusters with dual emissions and their application in biosensing

Tang

Chen²,

Wang

et al. 2022

J Nanobiotechnol

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“…A number of microorganisms have been characterized using functionalized SPCEs, including Escherichia coli, , Salmonella, Pseudomonas aeruginosa, S. aureus, − and Listeria. , However, most of these electrochemical biosensors are designed to use specific antibodies, which presents several drawbacks in terms of limited storage stability, large size, and partial inhibition due to the matrix effect or their orientation on the electrode surface. , Significant efforts are thus underway to develop biorecognition elements that are smaller and more stable.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection and Identification of Vancomycin-Sensitive Bacteria Using an Electrochemical Apta-Sensor

Novakovic,

Khalife,

Costache

et al. 2024

ACS Omega

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In order to combat the complex and diverse infections caused by bacteria, it is essential to develop efficient diagnostic tools. Current techniques for bacterial detection rely on laborious multistep procedures, with high costs and extended time of analysis. To overcome these limitations, we propose here a novel portable electrochemical biosensor for the rapid detection and identification of Gram-positive bacteria that leverages the recognition capabilities of vancomycin and aptamers. A vancomycin-modified screen-printed carbon electrode was used to selectively capture Gram-positive bacteria susceptible to this antibiotic. Electrochemical impedance spectroscopy and scanning electron microscopy demonstrated that capture was achieved in 10 min, with a limit of detection of only 2 CFU/mL. We then tested the device's potential for aptamer-based bacterial identification using Staphylococcus aureus and Bacillus cereus as the test strains. Specifically, electrodes with captured bacteria were exposed to species-specific aptamers, and the resulting changes in current intensity were analyzed using differential pulse voltammetry. When used directly in untreated milk or serum, the system was able to successfully identify a small amount of S. aureus and B. cereus (100 CFU/mL) in less than 45 min. This novel biosensor has the potential to serve as an invaluable tool that could be used, even by inexperienced staff, in a broad range of settings including clinical diagnostics, food safety analysis, environmental monitoring, and security applications.

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“…The use of electrochemistry for measurement of different medicine in various solution and furthermore other functional technologies has much attention has been paid to them. [19][20][21][22][23][24][25][26][27]. Among the different analytical methods, the electrochemical methods compared to other methods, are powerful detection method due to the intrinsic properties such as sensitivity, low-cost, portability and ease of use [16,[28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical codeine sensor based on carbon paste electrode/HKUST-1

Pirasteh

Isfahani

Pourghobadi

2022

Mater. Res. Express

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In this research, the Cu-MOF (metal-organic framework, HKUST-1) was synthesized via co-precipitation method and it was into the carbon paste electrode and has been investigated in the measurement of codeine. The electrochemical performance of the modified electrode was evaluated by cyclic voltammetry and differential pulse voltammetry. The effective parameters in the sensitivity of the method were optimized. Quantitative measurements and determination of codeine at the surface of the modified electrode were performed by using differential pulse voltammetry. Finally, the ability of the developed method to measure codeine in real plasma samples was investigated. Under the optimal conditions, the linear range was obtained from 2 to 100 μM with a limit of detection of 0.66 μM. The high efficiency of the developed electrode in plasma samples was proved by using high and acceptable accuracy and satisfactory relative recovery percentage. The results in which the recoveries values with RSD% for three repeated measurements were in the range of 97–109 (%RSD = 3.75 to 4).

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Architecting of an aptasensor for the staphylococcus aureus analysis by modification of the screen-printed carbon electrode with aptamer/Ag–Cs-Gr QDs/NTiO2

Cited by 37 publications

References 58 publications

Fabrication of avidin-stabilized gold nanoclusters with dual emissions and their application in biosensing

Fabrication of avidin-stabilized gold nanoclusters with dual emissions and their application in biosensing

Rapid Detection and Identification of Vancomycin-Sensitive Bacteria Using an Electrochemical Apta-Sensor

Electrochemical codeine sensor based on carbon paste electrode/HKUST-1

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