Arbutin Improves Functional Recovery and Attenuates Glial Activation in Lysolecethin-Induced Demyelination Model in Rat Optic Chiasm

Ebrahimtabar, Forough; Nazari, Atena; Pouramir, Mahdi; Ashrafpour, Manouchehr; Pourabdolhossein, Fereshteh

doi:10.1007/s12035-020-01962-x

Cited by 22 publications

(15 citation statements)

References 60 publications

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“…An optimal annealing temperature for each primer was mentioned in Table 2. The cycle threshold (CT) of target genes was normalized to GAPDH as the reference gene to minimize the effect of sample variations and calculate the relative expression level of the target genes by the 2-delta-delta CT method [37]. Biochemical assays…”

Section: Quantitative Real-time Pcr (Q-pcr)mentioning

confidence: 99%

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Piperine Improves Experimental Autoimmune Encephalomyelitis (EAE) in Lewis Rats Through its Neuroprotective, Anti-inflammatory, and Antioxidant Effects

et al. 2021

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In ammation, demyelination, glial activation, and oxidative damage are the most pathological hallmarks of multiple sclerosis (MS). Piperine, a main bioactive alkaloid of black pepper, possesses antioxidant, anti-in ammatory and neuroprotective properties whose therapeutic potential has been less studied in the experimental autoimmune encephalomyelitis (EAE) models. In this study, the e ciency of peprine on progression of EAE model and myelin repair mechanisms was investigated. EAE was induced in female Lewis rats and piperine and its vehicle were daily administrated intraperitoneally from day 8 to 29 post immunization. We found that peperine alleviated neurological de cits and EAE disease progression. Luxol fast blue and H&E staining and immuno-staining of lumbar spinal cord cross sections con rmed that piperine signi cantly reduced the extent of demyelination, in ammation and immune cell in ltration and inhibited microglia and astrocyte activation. Gene expression analysis in lumbar spinal cord showed that piperine treatment decreased the level of pro-in ammatory cytokines (TNF-α, IL-1β) and iNOS and enhanced IL-10, Nrf-2, HO-1and MBP expressions. Piperine supplementation also enhanced the total antioxidant capacity (FRAP) and reduced the level of oxidative stress marker (MDA) in the CNS of EAE rats. Finally, we found that piperine has anti-apoptotic and neuroprotective effect in EAE through reducing caspase-3 (apoptosis marker) and enhancing BDNF and NeuN expressing cells. This study strongly indicates that piperine has a bene cial effect on the EAE progression and could be considered as a potential therapeutic target for MS treatment. Upcoming clinical trials will provide the deep understanding of piperine's role for the treatment of the MS.

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Section: Quantitative Real-time Pcr (Q-pcr)mentioning

confidence: 99%

“…The immunostaining procedure was performed based on our previous study [37,47]. The lumbar spinal cord of rats was dissected after perfusion and post-xed 2 h in PFA (4%) and then incubated in 20% sucrose solution overnight.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Piperine Improves Experimental Autoimmune Encephalomyelitis (EAE) in Lewis Rats Through its Neuroprotective, Anti-inflammatory, and Antioxidant Effects

et al. 2021

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“…Arbutin attenuated the astrocyte marker GFAP in the brain. These results suggested that arbutin improves myelin repair and functional recovery of demyelination of the optic nerve by attenuating inflammation and oxidative stress [ 158 ].…”

Section: Natural Compounds Against Oxidative Stress In Animal Models ...mentioning

confidence: 99%

Potential Utility of Natural Products against Oxidative Stress in Animal Models of Multiple Sclerosis

Zha

Liu

et al. 2022

Antioxidants

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Multiple sclerosis (MS) is an autoimmune-mediated degenerative disease of the central nervous system (CNS) characterized by immune cell infiltration, demyelination and axonal injury. Oxidative stress-induced inflammatory response, especially the destructive effect of immune cell-derived free radicals on neurons and oligodendrocytes, is crucial in the onset and progression of MS. Therefore, targeting oxidative stress-related processes may be a promising preventive and therapeutic strategy for MS. Animal models, especially rodent models, can be used to explore the in vivo molecular mechanisms of MS considering their similarity to the pathological processes and clinical signs of MS in humans and the significant oxidative damage observed within their CNS. Consequently, these models have been used widely in pre-clinical studies of oxidative stress in MS. To date, many natural products have been shown to exert antioxidant effects to attenuate the CNS damage in animal models of MS. This review summarized several common rodent models of MS and their association with oxidative stress. In addition, this review provides a comprehensive and concise overview of previously reported natural antioxidant products in inhibiting the progression of MS.

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“…In various studies, it is revealed that arbutin formulations can be useful for the treatment of various diseases due to its multi‐target biological effects such as treatment of hyperpigmentation disorders (Ayumi, Sahudin, Hussain, Hussain, & Samah, 2019; Park et al, 2019), anti‐diabetic (Lv et al, 2019), wound healing (Polouliakh et al, 2020), anti‐osteoporosis (Bonifacio et al, 2020), treatment of acute lung injury (Ye et al, 2019), management of cardiac hypertrophy (Nalban et al, 2020), anticancer (Jiang et al, 2018; Safari et al, 2020; Wang, Wang, Li, Zhang, & Wang, 2020), antioxidant activity (Bonifacio et al, 2020), treatment of optic nerve injury (Ebrahim‐Tabar, Nazari, Pouramir, Ashrafpour, & Pourabdolhossein, 2020; Zhao, Wang, Qin, & Wang, 2019), treatment of central nervous system disorders (Ahmadian, Ghasemi‐Kasman, Pouramir, & Sadeghi, 2019; Dastan et al, 2019; Ding et al, 2020), hepatoprotective activity (Mirshahvalad et al, 2016), effect on human mesenchymal stem cells (Bonifacio et al, 2020; Jamil, Crowe, Tablin, & Oliver, 2005), radioprotective effect (Nadi, Elahi, Moradi, & Banaei, 2019), antimicrobial (Nadi et al, 2019), antiaging (Zhou et al, 2017), antitussive (Koul, Kumar, Yadav, & Jin, 2020), diuretic (Myagchilov, Mineev, Sokolova, Gerdasova, & Gorovoi, 2020), anti‐inflammatory (Lee & Kim, 2012; Zhou, Zhao, Li, & Reetz, 2019), inhibitory effects on melanin formation (Li, Du, & Du, 2018), estrogen‐like effects (Zeng et al, 2018), and cytoprotective effects (Seyfizadeh et al, 2012) etc.…”

Section: Chemical Characterizations and Therapeutic Effects Of Arbutin In Cosmetic And Pharmaceutical Preparationsmentioning

confidence: 99%

A comprehensive review of the therapeutic potential of α‐arbutin

Saeedi

Khezri

Zakaryaei

et al. 2021

Phytotherapy Research

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Cosmetic dermatology preparations such as bleaching agents are ingredients with skin‐related biological activities for increasing and improving skin beauty. The possibility of controlling skin hyperpigmentation disorders is one of the most important research goals in cosmetic preparations. Recently, cosmetics containing herbal and botanical ingredients have attracted many interests for consumers of cosmetic products because these preparations are found safer than other preparations with synthetic components. However, high‐quality trial studies in larger samples are needed to confirm safety and clinical efficacy of phytotherapeutic agents with high therapeutic index. Arbutin (p‐hydroxyphenyl‐β‐d‐glucopyranoside) is a bioactive hydrophilic polyphenol with two isomers including alpha‐arbutin (4‐hydroxyphenyl‐α‐glucopyranoside) and β‐arbutin (4‐hydroxyphenyl‐β‐glucopyranoside). It is used as a medicinal plant in phytopharmacy. Studies have shown that alpha‐arbutin is 10 times more effective than natural arbutin. A comparison of IC50 values showed that α‐arbutin (with concentration 2.0 mM) has a more potent inhibitory activity on human tyrosinase against natural arbutin (with higher concentration than 30 mM). A review of recent studies showed that arbutin could be beneficial in treatment of various diseases such as hyperpigmentation disorders, types of cancers, central nervous system disorders, osteoporosis, diabetes, etc. This study was designed to describe the therapeutic efficiencies of arbutin.

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Arbutin Improves Functional Recovery and Attenuates Glial Activation in Lysolecethin-Induced Demyelination Model in Rat Optic Chiasm

Cited by 22 publications

References 60 publications

Piperine Improves Experimental Autoimmune Encephalomyelitis (EAE) in Lewis Rats Through its Neuroprotective, Anti-inflammatory, and Antioxidant Effects

Piperine Improves Experimental Autoimmune Encephalomyelitis (EAE) in Lewis Rats Through its Neuroprotective, Anti-inflammatory, and Antioxidant Effects

Potential Utility of Natural Products against Oxidative Stress in Animal Models of Multiple Sclerosis

A comprehensive review of the therapeutic potential of α‐arbutin

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