Arbuscular mycorrhizal fungi stimulate organic phosphate mobilization associated with changing bacterial community structure under field conditions

Zhang, Lin; Shi, Ning; Jia, Fan; Wang, Fei; George, Timothy S.; Feng, Gu

doi:10.1111/1462-2920.14289

Cited by 122 publications

(95 citation statements)

References 54 publications

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“…The collected soil was air dried and sieved (2 mm). The basal nutrients were added to the soil as described in recruited a hyphosphere microbiome that has the potential to stimulate the solubility of organic P [6,7]. In this study, to enhance the colonization of soil microbiome in hyphosphere, 100 mg kg -1 myo-inositol hexaphosphate calcium magnesium salt (phytin, TCI, Tokyo, Japan) (equaling to 20 mg P kg -1 soil) was added to the hyphal compartment as an organic P resource.…”

Section: Soilmentioning

confidence: 99%

“…3, 4, 5 and 6). All these results suggested the microbiomes that associated with the three AM fungal species were distinct.Previous studies have indicated that the hyphosphere microbiome are directly involved in soil organic N, P, C mineralization[7,27,31,34,35]. For example,Pseudomonas and Bacillus are reported to have abilities to mobilize sparingly soluble P in soil(Table S5)[5,7].…”

mentioning

confidence: 99%

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Arbuscular Mycorrhizal Fungi Co-Colonizing on A Single Plant Root System Recruit Distinct Microbiomes

Zhou

Chai

Zhang

et al. 2020

Preprint

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Background: Plant roots are usually colonized by various arbuscular mycorrhizal (AM) fungal species which vary in morphological, physiological and genetic traits and constitute the mycorrhizal nutrient uptake pathway (MP) in addition to roots. Simultaneously, the extraradical hyphae of each AM fungus is associated with a community of bacteria. However, whether the community structure and function of microbiome on the extraradical hyphae would differ between the AM fungal species are mostly unknown. Methods: In order to understand the community structure and the predicted functions of the microbiome associated with different AM fungal species, a split-root compartmented rhizobox culturing system, which allowed us to inoculate two AM fungal species separately in two root compartments was used. We inoculated two separate AM fungal species combinations, Funneliformis mosseae ( F.m ) and Gigaspora margarita ( G.m ), Rhizophagus intraradices ( R.i ) and G. margarita, on a single root system of cotton . The hyphal exudate fed active microbiome was measured by combining 13 C-DNA stable isotope probing with Miseq sequencing. Results: We found different AM fungal species, that were simultaneously colonizing on a single root system, hosted distinct active microbiomes from one another. Moreover, the predicted potential functions of the different microbiomes were distinct. Conclusion: We conclude that the arbuscular mycorrhizal fungi component of the system is responsible for the recruitment distinct microbiomes in the hyphosphere. The potential significance of the predicted functions of the microbiome ecosystem services is discussed.

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Section: Soilmentioning

confidence: 99%

mentioning

confidence: 99%

Arbuscular Mycorrhizal Fungi Co-Colonizing on A Single Plant Root System Recruit Distinct Microbiomes

Zhou

Chai

Zhang

et al. 2020

Preprint

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“…Importantly, the relationship between AMF and bacteria in P utilization can be regulated by the C:P ratio in soil [44] ; reducing soil C:P ratio by adding starter P fertilizer to Pdeficient soils can stimulate the P-solubilizing capacity of bacteria and improve plant P uptake [45] . AMF hyphae recruit bacteria that produce alkaline phosphatase and perform the functions, which are absent in fungal hyphae, to enhance soil organic P mineralization in situ in the field [44,46] . Also, a recent study showed that the stimulating effect of AMF on hyphosphere bacteria can be influenced by various abiotic and biotic factors.…”

Section: Phosphorus In the Mycorrhizospherementioning

confidence: 99%

Innovations of phosphorus sustainability: implications for the whole chain

Shen¹,

Wang²,

Jiao³

et al. 2019

Front. Agr. Sci. Eng.

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Phosphorus (P) is a non-renewable resource, therefore ensuring global food and environmental security depends upon sustainable P management. To achieve this goal, sustainable P management in the upstream and downstream sectors of agriculture from mineral extraction to food consumption must be addressed systematically. The innovation and feasibility of P sustainability are highlighted from the perspective of the whole P-based chain, including the mining and processing of P rock, production of P fertilizers, soil and rhizosphere processes involving P, absorption and utilization of P by plants, P in livestock production, as well as flow and management of P at the catchment scale. The paper also emphasizes the importance of recycling P and the current challenges of P recovery. Finally, sustainable solutions of holistic P management are proposed from the perspective of technology improvement with policy support.

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“…Fungal hyphae provide a unique niche for bacterial colonization, in which bacteria benefit from their fungal partner by obtaining suitable carbon or energy sources from fungal exudates (1,2). In return the specific bacterial communities recruited by fungal hyphae may help the host fungi by accessing recalcitrant forms of soil nutrients and hence meet the host's nutrient requirements (3).…”

Section: Introductionmentioning

confidence: 99%

Different Effects of Soil Fertilization on Bacterial Community Composition in the Penicillium canescens Hyphosphere and in Bulk Soil

Zhang

Hao

García-Lemos

et al. 2020

Appl Environ Microbiol

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This study investigated the effects of long-term soil fertilization on the composition and potential for phosphorus (P) and nitrogen (N) cycling of bacterial communities associated with hyphae of the P-solubilizing fungus Penicillium canescens. Using a baiting approach, hyphosphere bacterial communities were recovered from three soils that had received long-term amendment in the field with mineral or mineral plus organic fertilizers. P. canescens hyphae recruited bacterial communities with a decreased diversity and an increased abundance of Proteobacteria relative to what was observed in soil communities. As core bacterial taxa, Delftia and Pseudomonas spp. were present in all hyphosphere samples irrespective of soil fertilization. However, the type of fertilization showed significant impacts on the diversity, composition, and distinctive taxa/operational taxonomic units (OTUs) of hyphosphere communities. The soil factors P (Olsen method), exchangeable Mg, exchangeable K, and pH were important for shaping soil and hyphosphere bacterial community compositions. An increased relative abundance of organic P metabolism genes was found in hyphosphere communities from soil that had not received P fertilizers, which could indicate P limitation near the fungal hyphae. Additionally, P. canescens hyphae recruited bacterial communities with a higher abundance of N fixation genes than found in soil communities, which might imply a role of hyphosphere communities for fungal N nutrition. Furthermore, the relative abundances of denitrification genes were greater in several hyphosphere communities, indicating an at least partly anoxic microenvironment with a high carbon-to-N ratio around the hyphae. In conclusion, soil fertilization legacy shapes P. canescens hyphosphere microbiomes and their functional potential related to P and N cycling. IMPORTANCE P-solubilizing Penicillium strains are introduced as biofertilizers to agricultural soils to improve plant P nutrition. Currently, little is known about the ecology of these biofertilizers, including their interactions with other soil microorganisms. This study shows that communities dominated by Betaproteobacteria and Gammaproteobacteria colonize P. canescens hyphae in soil and that the compositions of these communities depend on the soil conditions. The potential of these communities for N and organic P cycling is generally higher than that of soil communities. The high potential for organic P metabolism might complement the ability of the fungus to solubilize inorganic P, and it points to the hyphosphere as a hot spot for P metabolism. Furthermore, the high potential for N fixation could indicate that P. canescens recruits bacteria that are able to improve its N nutrition. Hence, this community study identifies functional groups relevant for the future optimization of next-generation biofertilizer consortia for applications in soil.

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Arbuscular mycorrhizal fungi stimulate organic phosphate mobilization associated with changing bacterial community structure under field conditions

Cited by 122 publications

References 54 publications

Arbuscular Mycorrhizal Fungi Co-Colonizing on A Single Plant Root System Recruit Distinct Microbiomes

Arbuscular Mycorrhizal Fungi Co-Colonizing on A Single Plant Root System Recruit Distinct Microbiomes

Innovations of phosphorus sustainability: implications for the whole chain

Different Effects of Soil Fertilization on Bacterial Community Composition in the Penicillium canescens Hyphosphere and in Bulk Soil

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