Arachidonic Acid Metabolism in Articular Chondrocytes

Nagao, Masafumi; Ishii, Seiichi; Kitamura, K; Akino, Toyoaki

doi:10.1097/00003086-199110000-00039

Cited by 7 publications

(3 citation statements)

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“…Another response produced by BK on chondrocytes is prostanoids production via activation of PLA 2 . In rabbit chondrocytes BK induces [ 3 H]arachidonic acid release to the same extent as reported for endothelial cells, keratinocytes, and aortic smooth muscle cells [106]. On the other hand, contrary to other cell systems, the output of AA catabolic sub-products seems to be limited in articular chondrocytes with respect to other cell phenotypes, inasmuch as the amount of released PGE 2 was only 5 % of the whole catabolized AA.…”

Section: Kinins Effects On Chondrocytessupporting

confidence: 66%

Knee osteoarthritis: a role for bradykinin?

Meini

Maggi

2008

Inflamm. res.

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Osteoarthritis (OA) is a painful and degenerating progressive disease of the joints which affects millions of patients worldwide. The cause of OA is largely unknown. Among the potential therapies for the symptomatic treatment of OA, the intra-articular administration of a specific bradykinin (BK) B2 receptor antagonist has been reported to produce a long lasting analgesic effect in patients affected by knee OA. BK is a vasodilator and inflammatory nonapeptide which is generated in OA synovium. It contributes to the initiation and maintenance of inflammation, to exciting and sensitizing sensory nerve fibres, thus producing pain, and to activating synoviocytes and chondrocytes which are the main cells involved in the homeostasis of synovial fluid and cartilage, respectively. Moreover, BK synergistically potentiates the effects produced by pro-inflammatory cytokines. Biochemical and preclinical evidence supporting the therapeutic relevance of B2 receptor blockade in OA pathophysiology are reviewed in this publication.

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Section: Kinins Effects On Chondrocytessupporting

confidence: 66%

Knee osteoarthritis: a role for bradykinin?

Meini

Maggi

2008

Inflamm. res.

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“…In fact, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin are major components of the articular cartilage boundary lubricant [ 46 ]. In addition to the presence of these phospholipids in the joint fluid, free fatty acids may be incorporated by chondrocytes into phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and triacylglycerol (TG) [ 41 , 47 ].…”

Section: Lipids In Normal Cartilagementioning

confidence: 99%

Lipid Transport and Metabolism in Healthy and Osteoarthritic Cartilage

Villalvilla

Gómez

Largo

et al. 2013

IJMS

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Cartilage is an avascular tissue and cartilage metabolism depends on molecule diffusion from synovial fluid and subchondral bone. Thus, nutrient availability is limited by matrix permeability according to the size and charge of the molecules. Matrix composition limits the access of molecules to chondrocytes, determining cell metabolism and cartilage maintenance. Lipids are important nutrients in chondrocyte metabolism and are available for these cells through de novo synthesis but also through diffusion from surrounding tissues. Cartilage status and osteoarthritis development depend on lipid availability. This paper reviews lipid transport and metabolism in cartilage. We also analyze signalling pathways directly mediated by lipids and those that involve mTOR pathways, both in normal and osteoarthritic cartilage.

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“…Phosphatidylcholines (PC) are an important product in lipid metabolism and closely related to the occurrence of inflammation [23]. Moreover, the feature metabolites of LysoPCs and PCs in IN closely related to PGE2 [24]. PGE2 is the vital substance that induces pyrexia [25].…”

Section: Discussionmentioning

confidence: 99%

Study on the antipyretic activity and potential mechanism of Indigo Naturalis on lipopolysaccharide-induced fever rat model

Xu,

Huang,

Luo

et al. 2023

Preprint

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Background As a Traditional Chinese Medicine with an antipyretic effect, Indigo Naturalis (IN) has a long history of application in China. It is often used with other heat-clearing drugs to treat high fever in children, which is different from other heat-clearing medicines. But its antipyretic effect and mechanism have not been fully elucidated yet. And the heat-clearing development of IN lacks convincing current evidence. Methods This study aimed to uncover the antipyretic effect and mechanism of IN on infectious fever by rectal temperature, pyrogen cytokines and metabolomics. Materials and Methods Firstly, the antipyretic effect of IN on LPS-induced fever rats was evaluated by the changes in rat rectal temperature. Then, the content of PGE2 and cAMP in the hypothalamus and TNF-α, IL-1β and IL-6 in the serum were determined by ELISA. Finally, the plasma and urine samples of febrile rats were analyzed in combination with UHPLC-Q-TOF-MS to explore the antipyretic mechanism of IN. Results Compared with pyrexia model rats, the rectal temperature of rats treated with Indigo Naturalis was suppressed after 0.5 h (P <0.01). The enzyme-linked immunosorbent assay (ELISA) showed that the expression of tumor necrosis TNF-α (P <0.01) and IL-6 (P <0.05) were suppressed by IN. A total of 11 endogenous metabolites were identified from the plasma samples of the IN group, the model group and the control group. Furthermore,15 metabolites were identified in the urine samples. Pathway analysis showed that IN mainly regulated glycerophospholipid metabolism, glucuronide mutual conversion and tryptophan metabolism. Conclusion This study clarified the exact effect of IN on infectious fever. It mainly works by regulating pyrogenic cytokines and glycerophospholipid pathways. The present research provided a deeper understanding of the traditional heat-clearing effect of IN. It provided a reference for the further scientific research of IN and the selection of antipyretic drugs in clinical practice.

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Arachidonic Acid Metabolism in Articular Chondrocytes

Cited by 7 publications

References 0 publications

Knee osteoarthritis: a role for bradykinin?

Knee osteoarthritis: a role for bradykinin?

Lipid Transport and Metabolism in Healthy and Osteoarthritic Cartilage

Study on the antipyretic activity and potential mechanism of Indigo Naturalis on lipopolysaccharide-induced fever rat model

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