Arachidonate metabolism in human placenta, fetal membranes, decidua and myometrium: lipoxygenase and cytochrome p450 metabolites as main products in HPLC profiles

Schäfer, Wolfgang; Zahradnik, H. P.; Arbogast, Eva; Wetzka, B.; Werner, Karin; Breckwoldt, M.

doi:10.1016/s0143-4004(96)90043-0

Cited by 46 publications

(12 citation statements)

References 19 publications

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“…The difference in the correlations of AA in maternal plasma versus placenta and in placenta versus fetal plasma is in accordance with studies reporting that, although there is a higher affinity for plasma membrane fatty acid-binding protein to bind AA than other PUFA [44], there is also a lower transfer of AA to the fetal side than of other fatty acids [42]. The limited capability of the placenta to transfer AA seems to be due to its accumulation of AA as an intermediate state before its conversion into thromboxane A 2 , eicosatrienoic and hydroxyeicosatetraenoic acids, which have been found in placenta [45][46][47]. In relation with AA in the placenta, it was also found here that the presence of AA is greater in phospholipids than in neutral lipids, different from DHA that is accumulated in a higher proportion in neutral lipids.…”

Section: Discussionmentioning

confidence: 99%

Influence of Changes in Dietary Fatty Acids during Pregnancy on Placental and Fetal Fatty Acid Profile in the Rat

Amusquivar

Herrera

2003

Neonatology

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To determine whether the composition of long-chain polyunsaturated fatty acids (PUFA) could be modified in the fetus by maternal dietary fatty acids, pregnant Sprague-Dawley rats were fed semipurified diets that differed only in the non-vitamin lipid component. The diets contained either 10 g palm, sunflower, olive or fish oil (FOD)/100 g diet. A total of 5–6 rats were studied in each group. At day 20 of gestation, corresponding to 1.5 days prior parturition, the fatty acids in maternal adipose tissue were closely related to the fatty acid composition in the corresponding diet. An important proportion of arachidonic acid (AA) appeared in maternal liver and plasma, although it was lower in the FOD than in the other groups. Except for saturated fatty acids, the proportion of individual fatty acids in the placenta correlated linearly with that in maternal plasma. Also, PUFA in fetal plasma and liver showed significant correlations with PUFA in maternal plasma. Again, AA showed the lowest proportion in the plasma and liver of the FOD group. Therefore, the maternal dietary fatty acid composition influences maternal and fetal plasma and tissue composition, and an increase in dietary ω–3 fatty acids decreases the amount of AA in maternal and fetal tissues.

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Section: Discussionmentioning

confidence: 99%

Influence of Changes in Dietary Fatty Acids during Pregnancy on Placental and Fetal Fatty Acid Profile in the Rat

Amusquivar

Herrera

2003

Neonatology

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“…Nevertheless, for the first time, the present data indicate the formation of lipoxygenase products by ovine embryos during the preimplantation period. Only few studies have reported lipoxygenase derivatives being synthesized by human or ovine placental tissues [45,46]. However, in spite of the fact that HETEs might be considered to be mediators in immunological processes at the feto-maternal interface, their exact role in pregnancy remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Cyclooxygenase-2 Unlike Cyclooxygenase-1 is Highly Expressed in Ovine Embryos during the Implantation Period1

Charpigny¹,

Reinaud²,

Tamby³

et al. 1997

Biology of Reproduction

110

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In this study we investigated expression of the two isoforms of the prostaglandin-forming enzyme, cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2), in sheep embryos. Using Western blot and immunohistochemical analyses, we demonstrated that Cox-2 was highly expressed in embryos from Day 8 to Day 17 of development whereas Cox-1 was undetectable during this time. The expression of Cox-2 was developmentally regulated. It was maximal between Days 14 and 16. There was a 30-fold increase in Cox-2 content per protein extract between Day 10 and Day 14, corresponding to a 50,000-fold increase in the whole embryo. The expression of Cox-2 declined after Day 16 to become undetectable by Day 25 of pregnancy. Cox-2 was localized in the trophoblastic cells and was not detected in the inner cell mass. The [3H]arachidonic acid metabolites synthesized by Cox-2-rich conceptuses were analyzed by HPLC after short-term embryo culture. Day 14 conceptuses released mainly cyclooxygenase metabolites and to a lesser extent lipoxygenase derivatives. Cyclooxygenase products were 6-keto-prostaglandin (PGF)1alpha 18.2% (+/- 4.2), thromboxane-B2 22.51% (+/- 15.9), PGF2alpha 21% (+/- 11), PGE2 14.5% (+/- 7.4), and PGD2 2.7% (+/- 2.6). Taken together, these results suggest an important role for the Cox-2-dependent cyclooxygenase metabolites during embryo development.

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“…As opposed to its murine equivalent, which is found only in macrophages [11], 15-LOX is an intracellular enzyme found in different tissues as blood vessels, endometrium [12] and placental tissue [13]. The 15-LOX overexpressor mice were developed on a genetic background of C57/B16/I129/SJL mice that were deficient in low density lipoprotein receptor (LDL-R -/-) to obtain two different strains: the 15-LOX/LDL-R -/-and the LDL-R -/- [14].…”

Section: Introductionmentioning

confidence: 97%

Reduced Reproduction with Increased Abortion Rate in Transgenic Mice That Overexpress 15-Lipoxygenase

Dar

Strassburger

Shaish

et al. 2001

Gynecol Obstet Invest

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15-Lipoxygenase (15-LOX) is a lipid-oxidizing enzyme that is involved in cell cycle regulation. To evaluate the effect of 15-LOX on reproduction, we studied transgenic mice that overexpress 15-LOX. The transgene was introduced over the genetic background of the low density lipoprotein receptor deficient mice (LDL-R^–/–) and reproduction was compared to LDL-R^–/– mice. We found a lower pregnancy rate in the 15-LOX/LDL-R^–/– mice as compared to the LDL-R^–/– mice (62.74 vs. 79.1%, p < 0.01). Additionally, a remarkably higher number of resorptions per pregnancy was found in the 15-LOX/LDL-R^–/– mice (16.7 vs. 3.27%, p < 0.001) and it was accompanied by a significantly higher activity of the 15-LOX enzyme in these resorptions as compared to other tissues. These findings may implicate a role for 15-LOX in the development of spontaneous abortions.

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Arachidonate metabolism in human placenta, fetal membranes, decidua and myometrium: lipoxygenase and cytochrome p450 metabolites as main products in HPLC profiles

Cited by 46 publications

References 19 publications

Influence of Changes in Dietary Fatty Acids during Pregnancy on Placental and Fetal Fatty Acid Profile in the Rat

Influence of Changes in Dietary Fatty Acids during Pregnancy on Placental and Fetal Fatty Acid Profile in the Rat

Cyclooxygenase-2 Unlike Cyclooxygenase-1 is Highly Expressed in Ovine Embryos during the Implantation Period1

Reduced Reproduction with Increased Abortion Rate in Transgenic Mice That Overexpress 15-Lipoxygenase

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