Arachidonate metabolism during chondrogenesisin vitro

Chepenik, Kenneth P.; Ho, Wai Chang; Waite, B. Moseley; Parker, Curtis L.

doi:10.1007/bf02405314

Cited by 37 publications

(11 citation statements)

References 33 publications

(25 reference statements)

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“…The effect of eicosanoids on collagenase‐1 gene expression was unique to PGE 1 because it was not elicited by the major endogenously synthesized cyclo‐oxygenase products PGE 2 or PGF 2α (33) . Both TNF and IL‐1, which induce metaloproteinase gene expression, also stimulate production of the major endogenous prostaglandins (PGE 2 and PGF 2α ) in synoviocytes, chondrocytes, and bone cells (44‐46) . However, blocking endogenous prostaglandin synthesis did not alter the ability of IL‐1 to increase collagenase‐1 gene expression (7,47,48) .…”

Section: Discussionmentioning

confidence: 99%

Role of Protein Kinase A in Collagenase-1 Gene Regulation by Prostaglandin E1: Studies in a Rabbit Synoviocyte Cell Line, HIG-82

Suzuki¹,

Rapuano²,

Bockman³

1997

Journal of Bone and Mineral Research

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Section: Discussionmentioning

confidence: 99%

Role of Protein Kinase A in Collagenase-1 Gene Regulation by Prostaglandin E1: Studies in a Rabbit Synoviocyte Cell Line, HIG-82

Suzuki¹,

Rapuano²,

Bockman³

1997

Journal of Bone and Mineral Research

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“…A developmental role is suggested by the presence of cPLA 2 activity in extracts of embryonic rat brain (27,28) and by the embryonic expression of cyclooxygenase, an enzyme that utilizes the AA produced by PLA 2 activity for prostaglandin synthesis (29,30). Eicosanoids have also been implicated in chondrogenic differentiation, bone metabolism, and palate development (31)(32)(33)(34). However, targeted gene inactivation in mice has failed to provide direct clues as to the developmental function of cPLA 2 activity.…”

mentioning

confidence: 99%

Characterization of Ca2+-dependent Phospholipase A2 Activity during Zebrafish Embryogenesis

Farber

Olson

Clark

et al. 1999

Journal of Biological Chemistry

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We have developed a simple fluorescent assay for detection of phospholipase A 2 (PLA 2 ) activity in zebrafish embryos that utilizes a fluorescent phosphatidylcholine substrate. By using this assay in conjunction with selective PLA 2 inhibitors and Western blot analysis, we identified the principal activity in zebrafish embryogenesis as characteristic of the Ca 2؉ -dependent cytosolic PLA 2 (cPLA 2 ) subtype. Embryonic cPLA 2 activity remained constant from the 1-cell stage until the onset of somitogenesis, at which time it increased sharply. This increase was preceded by the expression of a previously identified zebrafish cPLA 2 homologue (Nalefski, E.,

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“…397 398 OKEEFE ET Al,.levels of prostaglandin E, precedes chondrogenesis, and PGE, has been further demonstrated to directly stimulate the chondrogenesis of undifferentiated limb mesodermal cells through a CAMP-mediated mechanism. (5)(6)(7)(8) Prior work demonstrated prostaglandin synthetase activity in homogenates of growth plate cartilage,'') and recently, prostaglandins of the E series were identified in normal growth plate cartilage in nanomolar concentrations. ( l o ) Prostaglandin E, was found to increase cartilaginous tissue mass in a fracture healing and prostaglandins El, E,, and D, were shown to stimulate the accumulation of cAMP in monolayer cultures of chondrocytes isolated from rabbit ribs.…”

mentioning

confidence: 99%

Influence of prostaglandins on DNA and matrix synthesis in growth plate chondrocytes

O’Keefe

Crabb

Puzas

et al. 1992

Journal of Bone and Mineral Research

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Prostaglandins are locally produced in a number of tissues in response to a variety of stimuli, including local growth factors and systemic hormones. The present investigation characterizes prostaglandin effects on growth plate chondrocytes. Since cyclic adenosine monophosphate (cAMP) may act as a prostaglandin-stimulated second messenger, the effects of prostaglandins A1, D2, E1, E2, F2 alpha, and I2 (10(-10)-10(-6) M) on cAMP levels and thymidine incorporation were evaluated. The stimulation of cAMP and thymidine incorporation by the various prostaglandin metabolites were dose dependent and highly correlated (r = 0.99, p less than 0.001). The magnitude of the effect varied but was maximal at 10(-6) M for each of the prostaglandins. Prostaglandins of the E series (E1 and E2) were the most potent, causing significant effects at 10(-10) M and with maximal 12- and 13-fold increases in DNA synthesis after a 24 h exposure. Prostaglandins D2 and A1 maximally stimulated thymidine incorporation by 4.7- and 3.1-fold but caused significant increases only at 10(-8) M. Prostaglandins F2 alpha and I2 were the least stimulatory, producing small but significant increases in thymidine incorporation at 10(-6) M (30 and 100% stimulations). A causal relationship between cAMP and thymidine incorporation was further verified by the ability of dibutyryl-cAMP to increase DNA synthesis. Long-term chondrocyte cultures treated continuously with PGE2 demonstrated an increase in cell number, confirming the proliferative effect. Indomethacin did not alter the potent dose-dependent stimulations of chondrocyte DNA synthesis by TGF-beta 1, basic FGF, or PTH, indicating that these known mitogens act independently of prostaglandin metabolism. PGE2 was further examined for its effects of matrix synthesis. PGE2 inhibited collagen synthesis with a maximal 42% decrease but did not alter noncollagen protein synthesis. In contrast, PGE2 maximally increased sulfate incorporation by 35% and caused a small dose-dependent inhibition in alkaline phosphatase activity. Thus, prostaglandins alter DNA and matrix synthesis in growth plate chondrocytes and may have an important role in chondrocyte metabolism in the growth plate, fracture callus, and other areas of endochondral ossification.

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Arachidonate metabolism during chondrogenesisin vitro

Cited by 37 publications

References 33 publications

Role of Protein Kinase A in Collagenase-1 Gene Regulation by Prostaglandin E1: Studies in a Rabbit Synoviocyte Cell Line, HIG-82

Role of Protein Kinase A in Collagenase-1 Gene Regulation by Prostaglandin E1: Studies in a Rabbit Synoviocyte Cell Line, HIG-82

Characterization of Ca2+-dependent Phospholipase A2 Activity during Zebrafish Embryogenesis

Influence of prostaglandins on DNA and matrix synthesis in growth plate chondrocytes

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