Arabinogalactan proteins: Immunolocalization in the developing ovary of a facultative apomict Fragaria x ananassa (Duch.)

Leszczuk, Agata; Szczuka, Ewa

doi:10.1016/j.plaphy.2017.12.003

Cited by 17 publications

(18 citation statements)

References 40 publications

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“…Immunofluorescence labeling performed in these studies showed strong fluorescence near crystals, indicating the presence of AGP epitopes. AGPs are generally considered as proteoglycans involved in developmental processes (Showalter 2001;Seifert and Roberts 2007;Pereira et al 2015;Leszczuk and Szczuka 2018), but AGPs have never been observed in a close association with calcium oxalate crystals. Clearly, AGPs have a possibility to bind calcium ions and act as calcium capacitors, which have an impact on plant growth and development (Lamport and Várnai 2013).…”

Section: Discussionmentioning

confidence: 99%

The Occurrence of Calcium Oxalate Crystals and Distribution of Arabinogalactan Proteins (AGPs) in Ovary Cells During Fragaria x ananassa (Duch.) Development

Leszczuk

Wydrych

Szczuka

2019

J Plant Growth Regul

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In this study, the presence of calcium oxalate crystals and distribution of arabinogalactan proteins (AGPs) in the ovary of Fragaria x ananassa (Duchesne) were investigated. The arrangement of the AGP epitopes was analyzed using immunohistochemistry techniques with JIM13, JIM15, and MAC207 monoclonal antibodies. To confirm the immunofluorescence studies, experiments at the subcellular level were performed with the immunogold method using transmission electron microscopy. We observed calcium oxalate crystals as druses in the middle layer of the ovary, which created a distinct ring-like immunofluorescence pattern around the ovule, indicating the presence of AGP epitopes. We propose hypotheses concerning the roles of calcium oxalate crystals in ovaries: the occurrence of crystals in the external part of the ovary may constitute a mechanical barrier against herbivores (1) the presence of AGPs in close proximity to calcium oxalate crystals may support their role as a calcium depository, which is important for the developing embryo and seed coat formation as well as in the interaction between pollen tubes and female gametophyte during sexual plant reproduction (2).

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Section: Discussionmentioning

confidence: 99%

The Occurrence of Calcium Oxalate Crystals and Distribution of Arabinogalactan Proteins (AGPs) in Ovary Cells During Fragaria x ananassa (Duch.) Development

Leszczuk

Wydrych

Szczuka

2019

J Plant Growth Regul

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“…AGP localization, revealed using monoclonal antibodies such as JIM8 and JIM13, is dynamic during ovule development and varies between species. For example, although AGP epitopes are not detected in the Arabidopsis MeMC, AGPs are observed in the MeMC of strawberry (Szczuka and Leszczuk, 2017;Leszczuk and Szczuka, 2018) and Hieracium species (Juranic et al, 2018). Moreover, AGPs have been observed in the cell wall of the MeMCs in the crassinucelate ovule of A. cherimola and P. americana (Lora et al, 2017).…”

Section: Cell Wall Components May Allow Germline Cells To Enter Distimentioning

confidence: 99%

Establishing a framework for female germline initiation in the plant ovule

Lora

Yang

Tucker

2019

Journal of Experimental Botany

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Female gametogenesis in flowering plants initiates in the ovule, where a single germline progenitor differentiates from a pool of somatic cells. Germline initiation is a fundamental prerequisite for seed development but is poorly understood at the molecular level due to the location of the cells deep within the flower. Studies in Arabidopsis have shown that regulators of germline development include transcription factors such as NOZZLE/SPOROCYTELESS and WUSCHEL, components of the RNA-dependent DNA methylation pathway such as ARGONAUTE9 and RNADEPENDENT RNA POLYMERASE 6, and phytohormones such as auxin and cytokinin. These factors accumulate in a range of cell types from where they establish an environment to support germline differentiation. Recent studies provide fresh insight into the transition from somatic to germline identity, linking chromatin regulators, cell cycle genes, and novel mobile signals, capitalizing on cell type-specific methodologies in both dicot and monocot models. These findings are providing unique molecular and compositional insight into the mechanistic basis and evolutionary conservation of female germline development in plants.

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“…Wigg. [ 32 , 33 ], Quercus suber L. [ 40 ], Fragaria × ananassa Duchesne [ 41 ] and Pilosella officinarum Vaill. [ 33 ], AGPs are produced in the ovule before the pollen tube arrives.…”

Section: Discussionmentioning

confidence: 99%

“…Similar results are known for various species, e.g., Amaranthus hypochondriacus [ 30 ], Arabidopsis thaliana [ 31 ], Sida hermaphrodita (L.) Rusby [ 44 ], Pitcairnia encholirioides L.B.Sm. [ 45 ], Quercus suber [ 46 ], Fragaria x ananassa [ 41 ] and Annona cherimola [ 7 ]. There are species-specific differences in AGPs.…”

Section: Discussionmentioning

confidence: 99%

Spatio-Temporal Distribution of Cell Wall Components in the Placentas, Ovules and Female Gametophytes of Utricularia during Pollination

Płachno

Kapusta

Świątek

et al. 2021

IJMS

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In most angiosperms, the female gametophyte is hidden in the mother tissues and the pollen tube enters the ovule via a micropylar canal. The mother tissues play an essential role in the pollen tube guidance. However, in Utricularia, the female gametophyte surpasses the entire micropylar canal and extends beyond the limit of the integument. The female gametophyte then invades the placenta and a part of the central cell has direct contact with the ovary chamber. To date, information about the role of the placenta and integument in pollen tube guidance in Utricularia, which have extra-ovular female gametophytes, has been lacking. The aim of this study was to evaluate the role of the placenta, central cell and integument in pollen tube pollen tube guidance in Utricularia nelumbifolia Gardner and Utricularia humboldtii R.H. Schomb. by studying the production of arabinogalactan proteins. It was also determined whether the production of the arabinogalactan proteins is dependent on pollination in Utricularia. In both of the examined species, arabinogalactan proteins (AGPs) were observed in the placenta (epidermis and nutritive tissue), ovule (integument, chalaza), and female gametophyte of both pollinated and unpollinated flowers, which means that the production of AGPs is independent of pollination; however, the production of some AGPs was lower after fertilization. There were some differences in the production of AGPs between the examined species. The occurrence of AGPs in the placental epidermis and nutritive tissue suggests that they function as an obturator. The production of some AGPs in the ovular tissues (nucellus, integument) was independent of the presence of a mature embryo sac.

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Arabinogalactan proteins: Immunolocalization in the developing ovary of a facultative apomict Fragaria x ananassa (Duch.)

Cited by 17 publications

References 40 publications

The Occurrence of Calcium Oxalate Crystals and Distribution of Arabinogalactan Proteins (AGPs) in Ovary Cells During Fragaria x ananassa (Duch.) Development

The Occurrence of Calcium Oxalate Crystals and Distribution of Arabinogalactan Proteins (AGPs) in Ovary Cells During Fragaria x ananassa (Duch.) Development

Establishing a framework for female germline initiation in the plant ovule

Spatio-Temporal Distribution of Cell Wall Components in the Placentas, Ovules and Female Gametophytes of Utricularia during Pollination

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