Arabidopsis thaliana Contains Two Differentially Expressed Farnesyl-Diphosphate Synthase Genes

Cunillera, Núria; Arró, Montserrat; Delourme, Didier; Karst, Francis; Boronat, Albert; Ferrer, Albert

doi:10.1074/jbc.271.13.7774

Cited by 159 publications

(138 citation statements)

References 50 publications

(65 reference statements)

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“…As a result of the dual functionality of the FaNES1-encoded protein, we could simultaneously show the possibility of forming comparable and correlated amounts of both types of terpenoids strongly suggesting availability of GPP, the precursor for linalool in the mitochondria. FPS1L and its homolog FPS2 can actually catalyse the two sequential condensation reactions: IPP with first DMAPP to form GPP and GPP with the second IPP leading to FPP formation (Cunillera et al, 1996;Delourme et al, 1994). The results show that GPP apparently available allowing de novo linalool biosynthesis in substantial amounts, even if FPS1L is not over-expressed (in COX lines).…”

Section: Discussionmentioning

confidence: 88%

Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum

Houshyani

Assareh

Busquets

et al. 2013

Metabolic Engineering

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, Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum, Metabolic Engineering, http://dx.doi.org/10.1016/j. ymben.2012.10.002 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Discussionmentioning

confidence: 88%

Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum

Houshyani

Assareh

Busquets

et al. 2013

Metabolic Engineering

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“…Our results are consistent with previous reports. For instance, Cunillera et al [25] reported that FPPS catalyses the synthesis of farnesyl diphosphate (FPP) from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). This reaction is considered to be a rate-limiting step in isoprenoid biosynthesis.…”

Section: Transcriptional Responses To Meja Stimulation At Different Cmentioning

confidence: 99%

Methyl jasmonate induces triterpenoid biosynthesis in Inonotus baumii

Sun

Zou

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

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In this work, methyl jasmonate (MeJA), as an exogenous elicitor, was used to investigate the feasibility of enhancing triterpenoid biosynthesis in Inonotus baumii. The results showed that the appropriate concentration of MeJA to be added was 150 mmol/L, and the triterpenoid yield was 12.61 mg/g dry weight (DW), which was 4.05-fold higher than that in the control with water. Moreover, quantitative real-time polymerase chain reaction was performed to measure the transcript levels of several genes in the triterpenoid pathway, including those encoding hydroxy-3-methylglutaryl-Coenzyme A synthase (hmgs), hydroxy-3-methylglutaryl-Coenzyme A reductase (hmgr), farnesyl pyrophosphate synthase (fpps), squalene synthase (sqs), squalene epoxidase (se) and lanosterol synthase (ls). The results demonstrated that these genes were mostly up-regulated by MeJA, although the levels of induction differed. For hmgs, hmgr, fpps and se, the highest transcript levels were at 150 mmol/L MeJA, and were approximately 3.0, 2.5, 2.0 and 2.0-fold higher than those in the control, respectively. However, the sqs and ls genes appeared to be repressed by MeJA. To the best of our knowledge, this is the first time to assess the use of MeJA to elicit triterpenoid biosynthesis in I. baumii, and the results indicated that MeJA was indeed a potent inducer of triterpenoid biosynthesis.

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“…Typically, neutral to alkaline pH conditions have been used for in vitro prenyl transferase assays, e.g. FPPS from Arabidopsis and yeast (Saccharomyces cerevisiae) has been assayed at pH 7.0 (Cunillera et al, 1996) and 7.4 (Chambon et al, 1990;Okada et al, 2000). GGPPS from H. brasiliensis shows a broad pH optimum range of 8.0 to 9.0 (Takaya et al, 2003), and the polyprenyl diphosphate synthases from Mycobacterium spp.…”

Section: Effect Of Ph Variation On Isoprenoid Synthase Activitiesmentioning

confidence: 99%

“…A protocol to assay isoprenoid synthase activity was developed from a combination of the procedures of Cunillera et al (1996), Okada et al (1998Okada et al ( , 2000, and Fujii et al (1982). Briefly, 100 mL reaction mixtures containing cell-free extract (100 mg of protein) in 100 mM potassium phosphate, pH 7.4, 1 mM EDTA, 1 mM 2-mercaptoethanol, 5 mM MgCl 2 , 2.2 nmol of DMAPP, and 4.4 nmol of [4-14 C]IPP were incubated for 1 h at 37°C.…”

Section: In Vitro Isoprenoid Synthase Assaysmentioning

confidence: 99%

Maize cDNAs Expressed in Endosperm Encode Functional Farnesyl Diphosphate Synthase with Geranylgeranyl Diphosphate Synthase Activity

et al. 2006

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Isoprenoids are the most diverse and abundant group of natural products. In plants, farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP) are precursors to many isoprenoids having essential functions. Terpenoids and sterols are derived from FPP, whereas gibberellins, carotenoids, casbenes, taxenes, and others originate from GGPP. The corresponding synthases (FPP synthase [FPPS] and GGPP synthase [GGPPS]) catalyze, respectively, the addition of two and three isopentenyl diphosphate molecules to dimethylallyl diphosphate. Maize (Zea mays L. cv B73) endosperm cDNAs encoding isoprenoid synthases were isolated by functional complementation of Escherichia coli cells carrying a bacterial gene cluster encoding all pathway enzymes needed for carotenoid biosynthesis, except for GGPPS. This approach indicated that the maize gene products were functional GGPPS enzymes. Yet, the predicted enzyme sequences revealed FPPS motifs and homology with FPPS enzymes. In vitro assays demonstrated that indeed these maize enzymes produced both FPP and GGPP and that the N-terminal sequence affected the ratio of FPP to GGPP. Their functionality in E. coli demonstrated that these maize enzymes can be coupled with a metabolon to provide isoprenoid substrates for pathway use, and suggests that enzyme bifunctionality can be harnessed. The maize cDNAs are encoded by a small gene family whose transcripts are prevalent in endosperm beginning mid development. These maize cDNAs will be valuable tools for assessing the critical structural properties determining prenyl transferase specificity and in metabolic engineering of isoprenoid pathways, especially in cereal crops.

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Arabidopsis thaliana Contains Two Differentially Expressed Farnesyl-Diphosphate Synthase Genes

Cited by 159 publications

References 50 publications

Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum

Three-step pathway engineering results in more incidence rate and higher emission of nerolidol and improved attraction of Diadegma semiclausum

Methyl jasmonate induces triterpenoid biosynthesis in Inonotus baumii

Maize cDNAs Expressed in Endosperm Encode Functional Farnesyl Diphosphate Synthase with Geranylgeranyl Diphosphate Synthase Activity

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