Arabidopsis thaliana and omics approaches: a review

Abstract

Arabidopsis thaliana is a small, owering plant that is widely used as a model organism in plant biology, mainly because it is the rst plant to have its entire genome sequenced. It has since proven to be an ideal organism for studying plant development. Arabidopsis is commonly used as a model plant for genomics, metabolomics and proteomics studies, and more recently it has been utilized in metallomic studies. Because of its widespread applications, many methods for Arabidopsis sample preparation, analytes separation and data quanti cation have been explored. is review brie y describes the Arabidopsis thaliana characteristics, the developed researches and the primary methods using this plant in di erent elds of OMICS. In the future, the availability of Arabidopsis genomic information may result in its continuous development for nanoparticles and metallomics studies.

La participación de las cinasas de proteínas activadas por mitógenos en la señalización por hormonas en Arabidopsis thaliana L.

“…Entre las características que fomentaron el uso de Arabidopsis como organismo modelo destacan la facilidad para su cultivo en condiciones de laboratorio, por la disponibilidad de contar con protocolos de mutagénesis y métodos de selección de mutantes, además de un genoma pequeño con 5 cromosomas sobre los que se distribuyen alrededor de 25,000 genes en su mayoría de copia única, que ofrecen la posibilidad de transformarla genéticamente para regenerar plantas transgénicas estables, aunado al hecho de que es fácil mantener líneas homocigóticas estables debido a su baja tasa de entrecruzamiento (Woodward & Bartel, 2018). Adicionalmente, el genoma de Arabidopsis está totalmente secuenciado y el uso de técnicas de mutación dirigida mediante inserción de fragmentos de DNA, permitieron la identificación de la función de muchos de sus genes por genética reversa (Carneiro, Chacón-Madrid, Maciel & Zezzi, 2015). Finalmente, existen bancos de germoplasma encargados de recolectar, mantener y propagar las variantes genéticas de esta planta.…”

Functional Characterization of CNGC19 and CNGC20 of Arabidopsis through CRISPR-Cas9

“…The central loop of plants has 4 white petals, 6 stamens and gynoecium and upper whorl of plant contained 4 green sepals. Additionally, this plant is related to about eleven thousand gene families and sequence of genome comparable to protein encoding genes, size of nuclear genome is 125Mb [3]. It explains a brief story of sequence of chromosomes with observed evolutionary history.…”

Expression and in vitro function of anti-cancer mAbs in transgenic Arabidopsis thaliana

“…Among diverse plant platforms, Arabidopsis thaliana plant has several strengths such as a relatively short life span, high total soluble protein (TSP) yields, and cost-effective transformation methods (17)(18)(19). The endoplasmic reticulum (ER) retrieval motif has been fused to the C-terminus of the heavy chain (HC) of mAb thereby accumulation in ER retention signal peptide for high yields of anti-colorectal cancer mAb (4,13,20).…”