Arabidopsis <scp>PLC</scp>2 is involved in auxin‐modulated reproductive development

Lin, Li; He, Yuqing; Wang, Yarui; Zhao, Shujuan; Chen, Xi; Ye, Tiantian; Wu, Yuxuan; Wu, Yan

doi:10.1111/tpj.13016

Cited by 52 publications

(54 citation statements)

References 51 publications

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“…Studies in Arabidopsis indicate that PI‐PLCs play important roles in growth, development, and stress response. AtPLC2 is essential for seedling growth and reproductive development (Kanehara et al, ; Li et al, ). AtPLC3 and AtPLC9 positively regulate thermos‐tolerance in Arabidopsis (Liu et al, ; Zheng et al, ).…”

Section: Introductionmentioning

confidence: 99%

Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

Deng

Yuan

Cao

et al. 2018

Plant Cell & Environment

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Phosphatidylinositol-specific phospholipase C (PI-PLC) is involved in stress signalling but its signalling function remains largely unknown in crop plants. Here, we report that the PI-PLC4 from rice (Oryza sativa cv), OsPLC4, plays a positive role in osmotic stress response. Two independent knockout mutants, plc4-1 and plc4-2, exhibited decreased seedling growth and survival rate whereas overexpression of OsPLC4 improved survival rate under high salinity and water deficiency, compared with wild type (WT). OsPLC4 hydrolyses PI, phosphatidylinositol 4-phosphate (PI4P), and phosphatidylinositol-4,5-bisphosphate (PIP ) to generate diacylglycerol (DAG) in vitro. Knockout of OsPLC4 attenuated salt-induced increase of phosphatidic acid (PA) whereas overexpression of OsPLC4 decreased the level of PI4P and PIP under salt treatment. Applications of DAG or PA restored the growth defect of plc4-1 to WT but DAG kinase inhibitor 1 blocked the complementary effect of DAG in plc4-1 under salt stress. In addition, the loss of OsPLC4 compromised the increase of inositol triphosphate and free cytoplasmic Ca ([Ca ] ) and inhibited the induction of genes involved in Ca sensor and osmotic stress response to salt stress. The results indicate that OsPLC4 modulates the activity of two signalling pathways, PA and Ca , to affect rice seedling response to osmotic stress.

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Section: Introductionmentioning

confidence: 99%

Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

Deng

Yuan

Cao

et al. 2018

Plant Cell & Environment

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“…AtPLC1 regulates the expression of KIN1, RD29A and RD22 in response to ABA (Sanchez & Chua 2001). AtPLC2 modulates the biosynthesis of auxin during reproductive development by affecting the expression of YUC genes (Li et al 2015), and AtPLC2 also regulates the expression of BiP3, CNX and CRT in response to ER stress (Kanehara et al 2015). AtPLC3 and AtPLC9 regulate heat-shock protein (HSP) expression during heat shock (Liu et al 2006;Zheng et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…AtPLC1 is required for the secondary responses to ABA, and increased AtPLC1 activity is necessary for maximal gene induction by ABA (Sanchez & Chua 2001). AtPLC2 contributes to the ER stress response and auxin-modulated reproductive development (Kanehara et al 2015;Li et al 2015). AtPLC3 and AtPLC9 have an additive effect on regulating thermotolerance (Zheng et al 2012;Gao et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis phosphoinositide‐specific phospholipase C 4 negatively regulates seedling salt tolerance

Xia

Wang

Zhang

et al. 2017

Plant Cell & Environment

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Previous physiological and pharmacological studies have suggested that the activity of phosphoinositide-specific phospholipase C (PI-PLC) plays an important role in regulating plant salt stress responses by altering the intracellular Ca concentration. However, the individual members of plant PLCs involved in this process need to be identified. Here, the function of AtPLC4 in the salt stress response of Arabidopsis seedlings was analysed. plc4 mutant seedlings showed hyposensitivity to salt stress compared with Col-0 wild-type seedlings, and the salt hyposensitive phenotype could be complemented by the expression of native promoter-controlled AtPLC4. Transgenic seedlings with AtPLC4 overexpression (AtPLC4 OE) exhibited a salt-hypersensitive phenotype, while transgenic seedlings with its inactive mutant expression (AtPLC4m OE) did not exhibit this phenotype. Using aequorin as a Ca indicator in plc4 mutant and AtPLC4 OE seedlings, AtPLC4 was shown to positively regulate the salt-induced Ca increase. The salt-hypersensitive phenotype of AtPLC4 OE seedlings was partially rescued by EGTA. An analysis of salt-responsive genes revealed that the transcription of RD29B, MYB15 and ZAT10 was inversely regulated in plc4 mutant and AtPLC4 OE seedlings. Our findings suggest that AtPLC4 negatively regulates the salt tolerance of Arabidopsis seedlings, and Ca may be involved in regulating this process.

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“…Phosphoinositide-specific phospholipase C family protein (PLC), encoded by 7-1, was predicted to play an important role in signal transduction processes, mediating the cellular actions of a variety of hormones and growth factors. For example, PLC2 is involved in auxin biosynthesis and signaling, thus modulating the development of both male and female gametophytes in Arabidopsis [30]. The stress-induced Ca 2+ signaling, which is essential for controlling Na + accumulation in leaf blades, is dependent on phospholipase C (PLC) in Oryza sativa, thus establishing whole plant salt tolerance [31].…”

Section: Expression Analysis Of Erf and Interacting Protein Gene In Rmentioning

confidence: 99%

Screening of proteins interacting with ERF transcriptional factor from Populus simonii × P.nigra by yeast two-hybrid method

Wang

Xia

Jiang

2018

Biotechnology & Biotechnological Equipment

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To investigate the biology functions of ERF transcriptional factor in Populus simonii £ P.nigra, the proteins interacting with ERF were screened by the yeast two-hybrid method. The bait vector harbouring ERF and cDNA library of Populus simonii £ P.nigra were constructed. The bait had no self-activation and toxicity to yeast cells in the yeast two-hybrid system. The library titering experiment indicated that it could meet the requirement for the yeast two-hybrid library screen. Five proteins that interacted with ERF were obtained, including galactinol-sucrose galactosyl transferase 2, phosphoinositide-specific phospholipase C family protein, MLP-like protein, senescence-associated protein and an unknown protein. Real-time polymerase chain reaction showed that the expression levels of ERF and five interacting protein genes increased after salt treatment. Gene ontology analysis associated most of the interacting proteins with stress response. These results showed that ERF might share similar function with these proteins and they act together to regulate the salt response of Populus simonii £ P.nigra. This study provided experimental evidence for further revealing the biological function of the ERF transcription factor in poplar.

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Arabidopsis PLC2 is involved in auxin‐modulated reproductive development

Cited by 52 publications

References 51 publications

Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

Arabidopsis phosphoinositide‐specific phospholipase C 4 negatively regulates seedling salt tolerance

Screening of proteins interacting with ERF transcriptional factor from Populus simonii × P.nigra by yeast two-hybrid method

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