Arabidopsis lysin motif/F‐box‐containing protein InLYP1 fine‐tunes glycine metabolism by degrading glycine decarboxylase GLDP2

Abstract

Lysin motif (LysM) is a carbohydrate-binding module often found in secreted or transmembrane proteins in living organisms from prokaryotes to eukaryotes. Thus far, all characterized LysM-containing proteins in plants are plasma membrane-resident receptors or co-receptors playing roles in plant-microbe interactions. Here, we interrogate the Arabidopsis LysM/F-box-containing protein InLYP1 and reveal its function in glycine metabolism. InLYP1 was mainly expressed by vigorously growing tissues, encoding a nuclear-cytoplasmic protein. We validated InLYP1 as part of the SKP1-CULLIN1-F-box E3 complex for mediating protein degradation. The glycine decarboxylase P-protein 1 (GLDP1) was identified as an InLYP1-interacting protein by both immunoprecipitation/mass spectrometry and yeast two-hybrid library screening. InLYP1 could also interact with GLDP2, a paralog of GLDP1 with weaker catalytic activity, and could mediate the degradation of GLDP2 but not GLDP1. Interestingly, both GLDPs could be O-glycosylated and form homodimers or heterodimers. Overexpression of InLYP1 L9A encoding a dominant-negative variant could cause seedling germination retardation on the medium containing glycine. Collectively, these results shed light on the function of plant intracellular LysM-containing proteins, and suggest that InLYP1 may deplete GLDP2 to facilitate glycine decarboxylation in Arabidopsis.

Wheat Lysin-Motif-Containing Proteins Characterization and Gene Expression Patterns under Abiotic and Biotic Stress

“…LYP genes have been widely studied in a range of plants, including monocots and dicots [13]. Previous studies have shown that LysM domain containing proteins are involved in plant-microbe interactions, glycine metabolism, embryo sac development and other biological processes [39,40]. In this study, sixty-two LysM domain containing proteins were identified in wheat genome.…”

Comparative Transcriptome and Proteome Analysis Provides New Insights Into the Mechanism of Protein Synthesis in Kenaf (Hibiscus cannabinus L.) Leaves

“…Peroxidase participates in the photorespiration of plant cells and redox equilibrium reactions ( Jin et al, 2013 ). Glycine is mainly used as a glycine residue in leaf protein synthesis ( Guo et al, 2021 ). These modifications improved the efficiency of photosynthesis and provided raw materials for protein synthesis.…”

High light triggers flavonoid and polysaccharide synthesis through DoHY5‐dependent signaling in Dendrobium officinale

“…Dendrobium officinale protoplast isolation and transfection were performed as previously described (Wang, Xu, et al., 2021; Wang, Zhou, et al., 2021). Western blot analysis was conducted by combining two previously published protocols (Guo et al., 2021; Lei et al., 2019).…”