Arabidopsis gene expression patterns are altered during spaceflight

Paul, Anna‐Lisa; Popp, Michael P.; Gurley, William B.; Guy, Charles L.; Norwood, Kelly L.; Ferl, Robert J.

doi:10.1016/j.asr.2005.03.066

Cited by 74 publications

(47 citation statements)

References 41 publications

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“…A number of genome-scale studies of other plant species suggest that sHSPs are a part of the adaptive response to microgravity. [3][4][5][6]17 Based on these studies, it was concluded that plant cells respond to microgravity as if they were stressed and that the induction of some heat shock genes is a part of this response. 5 It was also proposed that the induction of sHSPs in microgravity plays a role in stabilizing the cytoskeleton and promoting cell signaling.…”

Section: Discussionmentioning

confidence: 99%

“…1,2 The development of modern methods such as microarrays and 2-D gel electrophoresis has enabled the study of the molecular basis of plant cellular responses to spaceflight. Studies have shown that microgravity [3][4][5][6] and its partial simulation by random positioning machines [7][8][9] and clinostats 10 in ground-based experiments, alters the function and transcriptional activity of multiple genes. Studies indicate that there are a number of genes that display moderate but highly significant changes in expression during spaceflight in Arabidopsis (Col-0) seedlings, [3][4][5] undifferentiated callus tissue 5 and single-cell model system of Ceratopteris richardii spores.…”

Section: Introductionmentioning

confidence: 99%

“…6 Among them are genes encoding proteins involved in pathogen defense and environmental stress responses. [3][4][5][6] One of the crucial elements of plant adaptation to unfavorable conditions is the so-called "protein quality control network" of molecular chaperones and proteases. Molecular chaperones are a group of diverse proteins that bind to other proteins that are in unstable conformations, thereby preventing their aggregation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expression of small heat shock protein (sHSP) genes in the garden pea (Pisum sativum) under slow horizontal clinorotation

Talalaiev

Korduym

2014

Plant Signaling & Behavior

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of small heat shock protein (sHSP) genes in the garden pea (Pisum sativum) under slow horizontal clinorotation

Talalaiev

Korduym

2014

Plant Signaling & Behavior

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“…17,18 Fundamental studies in higher plants such as Arabidopsis mainly pertain to their gravisensing properties. [19][20][21] But, by virtue of their recycling potential in life support systems, their growth behavior is also of direct relevance for manned missions (e.g., Micro Ecological Life Support System Alternative (MELISSA)). 22 Experiments with small organisms are typically expressed in terms of phenotypical changes.…”

Section: Biological Model Systems For Space Sciencementioning

confidence: 99%

Invited Review Article: Advanced light microscopy for biological space research

Vos

Beghuin²,

Schwarz

et al. 2014

Review of Scientific Instruments

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As commercial space flights have become feasible and long-term extraterrestrial missions are planned, it is imperative that the impact of space travel and the space environment on human physiology be thoroughly characterized. Scrutinizing the effects of potentially detrimental factors such as ionizing radiation and microgravity at the cellular and tissue level demands adequate visualization technology. Advanced light microscopy (ALM) is the leading tool for non-destructive structural and functional investigation of static as well as dynamic biological systems. In recent years, technological developments and advances in photochemistry and genetic engineering have boosted all aspects of resolution, readout and throughput, rendering ALM ideally suited for biological space research. While various microscopy-based studies have addressed cellular response to space-related environmental stressors, biological endpoints have typically been determined only after the mission, leaving an experimental gap that is prone to bias results. An on-board, real-time microscopical monitoring device can bridge this gap. Breadboards and even fully operational microscope setups have been conceived, but they need to be rendered more compact and versatile. Most importantly, they must allow addressing the impact of gravity, or the lack thereof, on physiologically relevant biological systems in space and in ground-based simulations. In order to delineate the essential functionalities for such a system, we have reviewed the pending questions in space science, the relevant biological model systems, and the state-of-the art in ALM. Based on a rigorous trade-off, in which we recognize the relevance of multi-cellular systems and the cellular microenvironment, we propose a compact, but flexible concept for space-related cell biological research that is based on light sheet microscopy.

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“…1,[3][4][5][6][7][8][9][10][11] However, many of these studies were conducted on plants germinated on the ground and transported into orbit for short periods, subjecting them to transient responses that may not have been due to the space environment. In this study, we planted and harvested barley in space and transported it to the ground in a frozen condition in order to eliminate hypergravity stress in space.…”

mentioning

confidence: 99%