Arabidopsis AtVPS15 Plays Essential Roles in Pollen Germination Possibly by Interacting with AtVPS34

Wang, Weiying; Zhang, Li; Xing, Shufan; Ma, Zhiqiang; Liu, Jingjing; Gu, Hongya; Qin, Genji; Liu, Qu

doi:10.1016/j.jgg.2012.01.002

Cited by 29 publications

(18 citation statements)

References 83 publications

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“…UVARG also forms a PI3K complex containing VPS34, Beclin1/VPS30 and VPS15 in mammals (Liang et al ., ; Takahashi et al ., ; Itakura et al ., ; Matsunaga et al ., ; Zhong et al ., ). In Arabidopsis , we previously found that AtVPS15 and AtVPS30 are essential for the development of male gametophytes (Qin et al ., ; Wang et al ., ). No homozygous mutants of atvps15 or atvps30 could be obtained in the progeny of the heterozygous +/ atvps15 or +/ atvps30 (Fujiki et al ., ; Qin et al ., ; Xu et al ., ; Wang et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…In Arabidopsis , we previously found that AtVPS15 and AtVPS30 are essential for the development of male gametophytes (Qin et al ., ; Wang et al ., ). No homozygous mutants of atvps15 or atvps30 could be obtained in the progeny of the heterozygous +/ atvps15 or +/ atvps30 (Fujiki et al ., ; Qin et al ., ; Xu et al ., ; Wang et al ., ). The disruption of AtVPS34 also causes male gametophytes to be lethal (Lee et al ., ), indicating that the PI3K complex is pivotal for plant reproduction.…”

Section: Discussionmentioning

confidence: 97%

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The Arabidopsis USL1 controls multiple aspects of development by affecting late endosome morphology

et al. 2018

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Summary The polar transport of auxin controls many aspects of plant development. However, the molecular mechanisms underlying auxin tranport regulation remain to be further elucidated.We identified a mutant named as usl1 (unflattened and small leaves) in a genetic screen in Arabidopsis thaliana. The usl1 displayed multiple aspects of developmental defects in leaves, embryogenesis, cotyledons, silique phyllotaxy and lateral roots in addition to abnormal leaves. USL1 encodes a protein orthologous to the yeast vacuolar protein sorting (Vps) 38p and human UV RADIATION RESISTANCE‐ASSOCIATED GENE (UVRAG). Cell biology, Co‐IP/MS and yeast two‐hybrid were used to identify the function of USL1.USL1 colocalizes at the subcellular level with VPS29, a key factor of the retromer complex that controls auxin transport. The morphology of the VPS29‐associated late endosomes (LE) is altered from small dots in the wild‐type to aberrant enlarged circles in the usl1 mutants. The usl1 mutant synergistically interacts with vps29. We also found that USL1 forms a complex with AtVPS30 and AtVPS34.We propose that USL1 controls multiple aspects of plant development by affecting late endosome morphology and by regulating the PIN1 polarity. Our findings provide a new layer of the understanding on the mechanisms of plant development regulation.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

The Arabidopsis USL1 controls multiple aspects of development by affecting late endosome morphology

et al. 2018

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“…The cDNA was diluted and used as a template for RT-PCR or quantitative RT-PCR. Quantitative RT-PCR was performed with three biological repeats using SYBR Green real-time PCR Master Mix (Toyobo) as described previously (Luo et al, 2011;Wang et al, 2012). PCR was performed at 95°C for 2 min, 94°C for 10 s, 55°C for 20 s, and 72°C for 20 s. The relative expression level of each gene was calculated using the 2 2DDCT (cycle threshold) method, and ACT8 was used as an internal control (Livak and Schmittgen, 2001).…”

Section: Pcr Analysis and Gene Expression Assaysmentioning

confidence: 99%

The TIE1 Transcriptional Repressor Links TCP Transcription Factors with TOPLESS/TOPLESS-RELATED Corepressors and Modulates Leaf Development inArabidopsis

et al. 2013

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Leaf size and shape are mainly determined by coordinated cell division and differentiation in lamina. The CINCINNATA (CIN)-like TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors are key regulators of leaf development. However, the mechanisms that control TCP activities during leaf development are largely unknown. We identified the TCP Interactor containing EAR motif protein1 (TIE1), a novel transcriptional repressor, as a major modulator of TCP activities during leaf development. Overexpression of TIE1 leads to hyponastic and serrated leaves, whereas disruption of TIE1 causes epinastic leaves. TIE1 is expressed in young leaves and encodes a transcriptional repressor containing a C-terminal EAR motif, which mediates interactions with the TOPLESS (TPL)/TOPLESS-RELATED (TPR) corepressors. In addition, TIE1 physically interacts with CIN-like TCPs. We propose that TIE1 regulates leaf size and morphology by inhibiting the activities of TCPs through recruiting the TPL/TPR corepressors to form a tertiary complex at early stages of leaf development.

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“…Pollen germination occurs rapidly during pollination, and is critical for successful sexual reproduction in plants. A series of cellular components are formed and a large number of genes are coordinately expressed in the pollen germination and growth process (Wang et al 2012). This increased amount of cellular activity results in an increased demand for mitochondrial respiration and TCA cycle-derived metabolic products.…”

Section: Discussionmentioning

confidence: 99%

CsPDC-E1α, a novel pyruvate dehydrogenase complex E1α subunit gene from Camellia sinensis, is induced during cadmium inhibiting pollen tube growth

Pan

Shu

et al. 2017

Can. J. Plant Sci.

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Arabidopsis AtVPS15 Plays Essential Roles in Pollen Germination Possibly by Interacting with AtVPS34

Cited by 29 publications

References 83 publications

The Arabidopsis USL1 controls multiple aspects of development by affecting late endosome morphology

The Arabidopsis USL1 controls multiple aspects of development by affecting late endosome morphology

The TIE1 Transcriptional Repressor Links TCP Transcription Factors with TOPLESS/TOPLESS-RELATED Corepressors and Modulates Leaf Development inArabidopsis

CsPDC-E1α, a novel pyruvate dehydrogenase complex E1α subunit gene from Camellia sinensis, is induced during cadmium inhibiting pollen tube growth

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