Arabidopsis AtNAP functions as a negative regulator via repression of AREB1 in salt stress response

Seok, Hye-Yeon; Woo, Dong-Hyuk; Nguyen, Linh Vu; Trần, Hương Thanh; Tarte, Vaishali N.; Mehdi, Syed Muhammad Muntazir; Lee, Sun-Young; Moon, Yong-Hwan

doi:10.1007/s00425-016-2609-0

Cited by 59 publications

(40 citation statements)

References 53 publications

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“…For instance, rice SNAC1 (Hu et al ), SNAC2 (Hu et al ), ONAC045 (Zheng et al ) and ONAC022 (Hong et al ) are positive regulators of the salt stress response. In the case of Arabidopsis, AtNAC2 (He et al ), NTL8 (Kim et al ), ATAF1 (Wu et al ), NTM2 (Park et al ), and AtNAP (Seok et al ) are key regulators in salt‐mediated plant development. However, functional identification of NACs has been rarely investigated in apple salt tolerance.…”

Section: Discussionmentioning

confidence: 99%

An apple NAC transcription factor enhances salt stress tolerance by modulating the ethylene response

Yao

et al. 2018

Physiologia Plantarum

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It is known that ethylene signaling is involved in the regulation of the salt stress response. However, the molecular mechanism of ethylene-regulated salt stress tolerance remains largely unclear. In this study, an apple NAM ATAF CUC transcription factor, MdNAC047, was isolated and functionally characterized to be involved in ethylene-modulated salt tolerance. MdNAC047 gene was significantly induced by salt treatment and its overexpression conferred increased tolerance to salt stress and facilitated the release of ethylene. Quantitative real-time-PCR analysis demonstrated that overexpression of MdNAC047 increased the expression of ethylene-responsive genes. Electrophoretic mobility shift assay, yeast one-hybrid and dual-luciferase assays suggested that MdNAC047 directly binds to the MdERF3 (ETHYLENE RESPONSE FACTOR) promoter and activates its transcription. In addition, genetic analysis assays indicated that MdNAC047 regulates ethylene production at least partially in an MdERF3-dependent pathway. Overall, we found a novel 'MdNAC047-MdERF3-ethylene-salt tolerance' regulatory pathway, which provide new insight into the link between ethylene and salt stress.

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Section: Discussionmentioning

confidence: 99%

An apple NAC transcription factor enhances salt stress tolerance by modulating the ethylene response

Yao

et al. 2018

Physiologia Plantarum

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“…Within a single flag leaf undergoing senescence, the expression of TaSNAC11-4B increased from the younger base to the yellowing tip ( Figure 1 B). Given that NAP TFs as well as a number of other NAC TF family members have been shown to be involved in stress responses [ 27 , 35 , 52 , 53 ], we examined the expression of TaSNAC11-4B in detached leaves after treatment with phytohormones ABA, MeJA, 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), indole-3-aceticacid (IAA), and stressors including NaCl and drought (PEG6000 mimic). Expression of the TaSNAC11-4B gene was induced by all the senescence-promoting phytohormones, ABA, JA, ethylene, and SA, with an increase of more than sixfold 12 h after ABA treatment ( Figure 1 C).…”

Section: Resultsmentioning

confidence: 99%

Wheat Transcription Factor TaSNAC11-4B Positively Regulates Leaf Senescence through Promoting ROS Production in Transgenic Arabidopsis

Zhang

Liu

Guo

2020

IJMS

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Senescence is the final stage of leaf development which is accompanied by highly coordinated and complicated reprogramming of gene expression. Genetic manipulation of leaf senescence in major crops including wheat has been shown to be able to increase stress tolerance and grain yield. NAC(No apical meristem (NAM), ATAF1/2, and cup-shaped cotyledon (CUC)) transcription factors (TFs) play important roles in regulating gene expression changes during leaf senescence and in response to abiotic stresses. Here, we report the characterization of TaSNAC11-4B (Uniprot: A0A1D5XI64), a wheat NAC family member that acts as a functional homolog of AtNAP, a key regulator of leaf senescence in Arabidopsis. The expression of TaSNAC11-4B was up-regulated with the progression of leaf senescence, in response to abscisic acid (ABA) and drought treatments in wheat. Ectopic expression of TaSNAC11-4B in Arabidopsis promoted ROS accumulation and significantly accelerated age-dependent as well as drought- and ABA-induced leaf senescence. Results from transcriptional activity assays indicated that the TaSNAC11-4B protein displayed transcriptional activation activities that are dependent on its C terminus. Furthermore, qRT-PCR and dual-Luciferase assay results suggested that TaSNAC11-4B could positively regulate the expression of AtrbohD and AtrbohF, which encode catalytic subunits of the ROS-producing NADPH oxidase. Further analysis of TaSNAC11-4B in wheat senescence and the potential application of this gene in manipulating leaf senescence with the purpose of yield increase and stress tolerance is discussed.

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“…Our data showed a similar trend as the two quinoa orthologues of ANAC083 were significantly induced by salt stress. AtNAP ( ANAC029) was identified as another NAC TF gene that is induced by salt stress (62). AtNAP functions as a negative regulator of salt stress response via repressing the expression of AREB1 and thus its overexpression increases the plants’ sensitivity to salt stress (62).…”

Section: Resultsmentioning

confidence: 99%

“…AtNAP ( ANAC029) was identified as another NAC TF gene that is induced by salt stress (62). AtNAP functions as a negative regulator of salt stress response via repressing the expression of AREB1 and thus its overexpression increases the plants’ sensitivity to salt stress (62). We found a similar expression trend in quinoa as the quinoa orthologue of AtNAP , AUR62005821 , was also induced by salt stress in both shoot and root.…”

Section: Resultsmentioning

confidence: 99%

Genome wide identification of NAC transcription factors and their role in abiotic stress tolerance in Chenopodium quinoa

Alshareef

Rey

Khoury

et al. 2019

Preprint

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Chenopodium quinoa Willd. (quinoa) is a pseudocereal with high nutritional value and relatively high tolerance to several abiotic stresses, including water deficiency and salt stress, making it a suitable plant for the study of mechanisms of abiotic stress tolerance. NAC (NAM, ATAF and CUC) transcription factors are involved in a range of plant developmental processes and in the response of plants to biotic and abiotic stresses. In the present study, we perform a genome-wide comprehensive analysis of the NAC transcription factor gene family in quinoa. In total, we identified 107 quinoa NAC transcription factor genes, distributed equally between sub-genomes A and B. They are phylogenetically clustered into two major groups and 18 subgroups. Almost 75% of the identified CqNAC genes were duplicated two to seven times and the remaining 25% of the CqNAC genes were found as a single copy. We analysed the transcriptional responses of the identified quinoa NAC TF genes in response to various abiotic stresses. The transcriptomic data revealed 28 stress responsive CqNAC genes, where their expression significantly changed in response to one or more abiotic stresses, including salt, water deficiency, heat and phosphate starvation. Among these stress responsive NACs, some were previously known to be stress responsive in other species, indicating their potentially conserved function in response to abiotic stress across plant species. Six genes were differentially expressed specifically in response to phosphate starvation but not to other stresses, and these genes may play a role in controlling plant responses to phosphate deficiency. These results provide insights into quinoa NACs that could be used in the future for genetic engineering or molecular breeding.

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Arabidopsis AtNAP functions as a negative regulator via repression of AREB1 in salt stress response

Cited by 59 publications

References 53 publications

An apple NAC transcription factor enhances salt stress tolerance by modulating the ethylene response

An apple NAC transcription factor enhances salt stress tolerance by modulating the ethylene response

Wheat Transcription Factor TaSNAC11-4B Positively Regulates Leaf Senescence through Promoting ROS Production in Transgenic Arabidopsis

Genome wide identification of NAC transcription factors and their role in abiotic stress tolerance in Chenopodium quinoa

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