Arabidopsis and rice showed a distinct pattern in ZIPs genes expression profile in response to Cd stress

Abstract

BackgroundPlant ZIP genes represent an important transporter family involved in metal transport. Evidence has implied that some ZIPs may contribute to plant Cd uptake, but a genome-wide examination of ZIPs’ role in Cd tolerance and uptake has rarely been reported. In this study, a genome-wide bioinformatic screening of candidate ZIP genes in Arabidopsis and rice was performed, followed by a systematic determination of their expression profile in response to Cd stress. Typical up-regulated ZIPs genes were then expressed in yeast cells to examine their effect on hosts’ Cd uptake.ResultsA total of 27 ZIP genes in Arabidopsis and rice were screened out based on sequence similarity. In Arabidopsis, Cd exposure strongly impacted the expression of most ZIPs, among which AtIRT1, AtIRT2, AtIRT4 AtZIP9, AtZIP10 and AtZIP12 were sharply up-regulated and AtIRT3, AtIRT5 were significantly down-regulated in root. In rice, all tested genes in shoot except for OsIRT1 and OsIRT12 were sharply up-regulated, while OsIRT1 and OsZIP1 in root were significantly down-regulated. Interestingly, some genes like AtIRT3, AtZIP5, AtZIP12, OsIRT1 and OsZIP1 showed converse expression regulation when subject to the tested Cd stress. When expressed in yeast cells, three ZIPs, AtIRT1, OsZIP1 and OsZIP3, caused a substantial increase in Cd sensitivity and Cd accumulation of the host cells.ConclusionsIn conclusion, this study revealed a distinct pattern in ZIPs family genes expression between Arabidopsis and rice in response to Cd stress. Arabidopsis mainly up-regulated root ZIPs genes, while rice mainly up-regulated shoot ZIPs genes. Three genes, AtIRT1, OsZIP1 and OsZIP3, conferred an increased Cd accumulation and sensitivity to Cd stress when expressed in yeast cells, further implying their roles in Cd uptake in plants.Electronic supplementary materialThe online version of this article (10.1186/s40529-018-0238-6) contains supplementary material, which is available to authorized users.

Potential of microbes in the biofortification of Zn and Fe in dietary food grains. A review

“…In nongraminaceous monocots and dicots (strategy I plants), phenolic compounds are the most frequently reported as a component of the root exudates produced in response to Fe Lin et al (2009), Varotto et al (2002), Vert et al (2002Vert et al ( , 2009, Zheng et al (2018)…”

“…Transport Fe, Zn, and Cd Curie et al (2000), Thomine et al (2000), Zheng et al (2018) AtNRAMP3 Transport Fe, Mn, and Cd; mobilization of vacuolar Fe stores; export of vacuolar Mn into photosynthetic tissues of adult plants Curie et al (2000), Lanquar et al (2010), Thomine et al (2000) AtNRAMP4…”

“…Iron efflux across plasma membrane; loading of Fe into xylem Morrissey et al (2009) AtFPN2 AtIREG2Influx of transition metals into vacuole; sequestration of toxic metals during Fe deficiency Morrissey et al (2009), Schaaf et al (2006) Oryza sativa OsIRT1/OsIRT2 Fe and Zn transportation Ishimaru et al (2006), Zheng et al (2018) OsNRAMP5…”

“…Moreover, phenolics such as protocatechuic acid (PCA) are reported to chelate Fe (III) and solubilize and reduce it to Fe (II) in vitro (Yoshino and Murakami 1998). Moreover, many rhizosphere beneficial microbes that elicit the induced systemic resistance (ISR) improve Fe and perhaps Zinc transporter in Arabidopsis Grotz et al (1998), Ivanov and Bauer (2017), Krämer et al (2007), Milner et al (2013, Zheng et al (2018) AtHMA2, AtHMA3, and AtHMA4…”

“…Functional Zn transporters Ivanov and Bauer (2017), Ramesh et al (2003), Zheng et al (2018) Hordeum vulgare Zn acquisition in strategy I plants (in strategy II plants, this has been less studied), through the induction of Fe deficiency responses (Romera et al 2019;Verbon et al 2017). The ISR and the Fe uptake signaling pathways interact in plant roots via the transcription factor MYB72, which controls the biosynthesis of Fe-mobilizing phenolics.…”