Aquimarina agarilytica sp. nov., an agarolytic species isolated from a red alga

Abstract

A novel yellow-pigmented, agarolytic bacterial strain, designated ZC1 T , was isolated from the surface of the marine red alga Porphyra haitanensis collected near Nan Ao Island, Guangdong province, China. The isolate was Gram-stain-negative, strictly aerobic and rod-shaped and displayed b-galactosidase, alkaline phosphatase, catalase and oxidase activities. The predominant cellular fatty acids were iso-C 15 : 0 , summed feature 3 (comprising C 16 : 1 v7c and/or iso-C 15 : 0 2-OH) and iso-C 17 : 0 3-OH. The major menaquinone was menaquinone 6 (MK-6). The DNA G+C content was 32.8 mol%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain ZC1T was closely related to members of the genus Aquimarina in the family Flavobacteriaceae, phylum Bacteroidetes. Based on phylogenetic and phenotypic evidence, strain ZC1 T (5CCTCC AB 2010229 T 5NBRC 107695 T ) represents the type strain of a novel species in the genus Aquimarina, for which the name Aquimarina agarilytica sp. nov. is proposed.The genus Aquimarina (family Flavobacteriaceae, phylum In the course of a screening of marine environments for agardegrading bacteria, a yellow-pigmented bacterium, designated strain ZC1 T , was isolated from the surface of a red alga (Porphyra haitanensis) collected from shallow water near the coast of Nan Ao Island, located on the Tropic of Cancer, at 117 u E, near the city of Shantou in Guangdong province, in south-eastern China. The red alga was crushed in a mortar with sterile seawater and spread on plates of marine agar (MA) consisting of 1.5 % agar, 0.5 % tryptone (Oxoid) and 0.1 % yeast extract (Oxoid) in artificial seawater containing (w/v) 2.5 % NaCl, 0.63 % MgSO 4 . 7H 2 O, 0.46 % MgCl 2 . 6H 2 O, 0.1 % CaCl 2 and 0.07 % KCl. The plates were incubated at 25 u C for 3 days under aerobic conditions. Strain ZC1 T , which formed colonies that sank into the agar and were each surrounded by a clear halo of liquid, was purified by successive streaking on MA. The purified strain was preserved at 280 u C in marine broth (MB) containing 15 % (v/v) glycerol.For DNA extraction, strain ZC1T was cultivated aerobically in MB supplemented with 0

Aquimarina hainanensis sp. nov., isolated from diseased Pacific white shrimp Litopenaeus vannamei larvae

“…At the time of writing, 17 species of the genus Aquimarina have been recognized or reclassified from other genera. All were isolated from the marine environment: seven from seawater, Aquimarina muelleri (Nedashkovskaya et al, 2005), A. latercula (Lewin 1969;Nedashkovskaya et al, 2006), A. addita (Yi & Chun, 2011), A. longa (Yu et al, 2013), A. megaterium , A. atlantica (Li et al, 2014a) and A. pacifica ; two from sediment, A. brevivitae (Yoon et al, 2006;Nedashkovskaya et al, 2006) and A. macrocephali (Miyazaki et al, 2010); two from mussels, A. mytili and A. gracilis (Park et al, 2013); two from marine sponges, A. spongiae (Yoon et al, 2011) and A. amphilecti (Kennedy et al, 2014); one from sea urchin, A. intermedia (Nedashkovskaya et al, 2006); one from the surface of the marine red alga Porphyra haitanensis, A. agarilytica (Lin et al, 2012); one from saltpan, A. salinaria (Chen et al, 2012); and one from Pacific white shrimp (Penaeus vannamei), A. penaei (Li et al, 2014b). One novel bacterial strain, designated M124 T , was isolated from diseased Pacific white shrimp Litopenaeus vannamei larvae.…”

Aurantiacicella marina gen. nov., sp. nov., a myxol-producing bacterium from surface seawater

“…Cells are Gram-stain-negative, non-motile, non-gliding, aerobic (Chen et al, 2012;Lewin, 1969;Li et al, 2014;Lin et al, 2012;Miyazaki et al, 2010;Nedashkovskaya et al, 2005Nedashkovskaya et al, , 2006Park et al, 2012Park et al, , 2013Yi & Chun, 2011;Yoon et al, 2006Yoon et al, , 2011Yu et al, 2013Yu et al, , 2014Zhang et al, 2014) Description of Aurantiacicella marina sp. nov.…”

“…S2. The major polar lipids of strain 2A-8 T were phosphatidylethanolamine, an unidentified aminolipid and five unidentified lipids; five more unidentified lipids, two unidentified aminolipids, lysophosphatidylethanolamine (phosphatidylethanolamine where one fatty acid chain is missing from the glycerol backbone) and an Nedashkovskaya et al, 2005); 3, A. addita JC2680 T , A. agarilytica ZC1 T , A. atlantica 22II-S11-z7 T , A. gracilis PSC32 T , A. intermedia KMM 6258 T , A. latercula LMG 1343 T , A. longa SW024 T , A. macrocephali JAMB N27 T , A. megaterium XH134 T , A. mytili PSC33 T , A. pacifica SW150 T , A. salinaria antisso-27 T , A. spongiae A6 T and A. brevivitae SMK-19 T (Chen et al, 2012;Li et al, 2014;Lin et al, 2012;Miyazaki et al, 2010;Nedashkovskaya et al, 2006;Park et al, 2012Park et al, , 2013Yi & Chun, 2011;Yoon et al, 2006Yoon et al, , 2011Yu et al, 2013Yu et al, , 2014Zhang et al, 2014); 4, Dokdonia donghaensis DSW-1 T and DSW-21 ; 5, D. diaphoros MSKK-32 T , D. eikasta PMA-26 T and D. genika Cos-13 T (Khan et al, 2006). Values are percentages of the total fatty acids and are shown when present at $0.5 %.…”

Aquimarina aggregata sp. nov., isolated from seawater

“…Subsequently, there has been a tremendous expansion in the number of species of the genus Aquimarina in the last few years. Eighteen species of the genus Aquimarina with validly published name have been identified at the time of writing, all from marine environments: Aquimarina latercula (Lewin, 1969;Nedashkovskaya et al, 2005), Aquimarina muelleri (Nedashkovskaya et al, 2005), Aquimarina addita (Yi & Chun, 2011), Aquimarina longa (Yu et al, 2013), Aquimarina megaterium , Aquimarina pacifica and Aquimarina atlantica (Li et al, 2014a) from seawater, Aquimarina brevivitae (Nedashkovskaya et al, 2006;Yoon et al, 2006) and Aquimarina macrocephali (Miyazaki et al, 2010) from sediment, Aquimarina intermedia (Nedashkovskaya et al, 2006) from a sea urchin, Aquimarina spongiae (Yoon et al, 2011) and Aquimarina amphilecti (Kennedy et al, 2014) from marine sponges, Aquimarina agarilytica (Lin et al, 2012) from the surface of the marine red alga Porphyra haitanensis, Aquimarina salinaria (Chen et al, 2012) from a saltpan, Aquimarina agarivorans (Zhou et al, 2015) from Gelidium amansii, Aquimarina mytili and Aquimarina gracilis (Park et al, 2013) from the mussel Mytilus coruscus and Aquimarina penaei (Li et al, 2014b) from Pacific white shrimp. In addition, the name of the species described as 'Aquimarina litoralis' (Oh et al, 2010) has not been yet been validly published.…”