Aqueous Ultrasound-Assisted Extraction for the Determination of Fluoroquinolones in Mangrove Sediment by High-Performance Liquid Chromatography and Fluorescence Detector

Abstract

A method for simultaneous analysis of ciprofloxacin (CIP), levofloxacin (LEV) and norfloxacin (NOR) in mangrove sediment was developed using aqueous ultrasound-assisted extraction (UAE), solid phase extraction (SPE) and high-performance liquid chromatography with fluorescence detection (HPLC-FD). The method showed excellent linear correlation coefficient for the three fluoroquinolones (r 2 > 0.999) using external calibration curve and good recovery in real sediment samples ranging from 73.73 to 88.85%. The precision showed a relative standard deviation lower than 20% and detection limits of 1.10, 3.33 and 0.26 µg kg -1 to CIP, LEV and NOR, respectively. There was no presence of interfering. After validation, the method was applied in the determination of pharmaceuticals in mangrove sediment in the Paciência River, Maranhão Island, Brazil. Only the CIP antibiotic was found in three of six samples analyzed, with values ranging 56.55-70.45 µg kg -1 .

Quantitative Determination of Fluoroquinolones in Contaminated Soils by HPLC with Solid-Phase Extraction

“…For the optimization of this variable, a series of different volume (5, 10, 15, 20, 25, 30 ml) of the solvent mixture (acetone: MeOH:formic acid (40:40:20) was investigated. The results showed that 10ml of extractant resulted in a maximal and unchangeable extraction yield which was in agreement with many studies which demonstrated the efficiency of the selected time on extraction yields (29,30). As a result, 10ml of extractant was adopted for the following analyses.…”

Ultrasound-assisted dispersive liquid-liquid microextraction for determination of enrofloxacin in surface waters

“…Therefore, the use of HPLC-FLD [14,20] has been chosen as an alternative due to the fact that is faster, easier to use and more accessible in common laboratories. Despite the use of a robust technique such as UHPLC-MS and HPLC-MS, preconcentration and cleaning techniques are still necessary to improve the analytical performance [21].…”

Fiberoptic-Coupled Spectrofluorometer with Array Detection as a Process Analytical Chemistry Tool for Continuous Flow Monitoring of Fluoroquinolone Antibiotics

“…Fluoroquinolones are among the most important antibacterial agents which were developed in the 80s and are now widely used in medicine and veterinary medicine [49]. erefore, several analytical procedure has been reported for determination of fluoroquinolones including high-performance liquid chromatography (HPLC) [50][51][52][53][54][55][56][57][58][59][60][61][62], spectrophotometry [63], fluorimetry [64][65][66][67][68][69][70][71][72][73], flow-injection based on chemiluminescence [74][75][76], chemiluminescence [77,78], terbium-sensitized luminescence [79], solid-phase spectrofluorimetry [80,81], capillary electrophoresis [82,83], convenient magnetic solid-phase extraction procedure coupled with capillary electrophoresis [84], solid-phase microextraction coupled with liquid chromatography-tandem mass spectrometry [85], liquid-liquid microextraction [86], polarography [87], and colorimetry [88] But, there is no report for application of PAT or PAC for online monitoring of fluoroquinolones.…”