Aqueous two-phase systems for protein separation: A perspective

Asenjo, Juan A.; Andrews, Bárbara A.

doi:10.1016/j.chroma.2011.06.051

Cited by 415 publications

(340 citation statements)

References 45 publications

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“…It is low cost, rapid, possess reusable polymers (Johansson et al 2011), scalable (Asenjo and Andrews 2011) and can withstand high biomass load as compared to rest of the separation systems (Kaul 2001). ATPS is reported as a challenging technique for the separation of molecules that are hard to be separated through other methods and preferred to be used in initial stages (Gupta et al 1999).…”

Section: Aqueous Two Phase System (Atps)mentioning

confidence: 99%

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Manzoor

Nawaz

Mukhtar

et al. 2016

Braz. arch. biol. technol.

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Section: Aqueous Two Phase System (Atps)mentioning

confidence: 99%

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Manzoor

Nawaz

Mukhtar

et al. 2016

Braz. arch. biol. technol.

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“…ATPS have many advantages such as low toxic to environment, continuous to operate, and easy to scale up (Hatti-kaul 2001). It is one of low cost and high-efficient purification methods (Asenjo and Andrews 2011). Recently, many enzymes (α-amylase, α-galactosidase, lipase, proteinase), recombinant proteins, and antibody have been purified using this method (Azevedo et al 2009;Porfiri et al 2011;Gu et al 2012;Loc et al 2013;Ramakrishnan et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Aqueous two-phase systems (ATPS) are formed by mixing certain amounts of polymers, salts, and water together, which have been extensively used to separate and purify nuclear acids, cells and organelles, enzymes, and proteins (Asenjo and Andrews 2011;Raja et al 2012). ATPS have many advantages such as low toxic to environment, continuous to operate, and easy to scale up (Hatti-kaul 2001).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterizations of a novel recombinant Bacillus velezensis endoglucanase by aqueous two-phase system

Liu

Guo

et al. 2018

Bioresour. Bioprocess.

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Background: Cellulases played an important role in the production of bioenergy and bio-products. Cellulases from bacteria with some special characteristics drew great attention due to its fast growth speed, wide adaption to harsh environment, and production of multi-function cellulases.Results: An endoglucanase gene egls from Bacillus velezensis A4 was cloned and expressed in Escherichia coli BL21 (DE3). The recombinant enzyme Egls was partially purified using aqueous two-phase system. The highest recovery rate of the enzyme was 90.39% at PEG 4000 (25% w/w), phosphate buffer 8.08% (w/w) (pH 6.0), and NaCl (5% w/w). The enzyme molecular weight was 55 KD estimated by zymogram. The optimal pH and temperature of recombinant enzyme Egls were pH 6.0 and 55 °C, respectively. The enzyme was stable at pH range of 5.0-7.0 at 55 °C for 60 min. The enzyme exhibited K m , V max , K cat values as 63.38 mg/ml, 55.6 mg/min, and 3.93 × 10 3 /S, respectively. The addition of 10 mM of Mg 2+ , Mn 2+ , or 5% (w/w) of Triton-X 100 in the reaction system enhanced the enzyme activity significantly. The enzyme showed both endoglucanase and exoglucanase activity. Conclusions:An endoglucanase gene egls from B. velezensis A4 was cloned and expressed in E. coli BL21 (DE3). The recombinant enzyme Egls was purified by aqueous two-phase system and characterized. The enzyme can be applied for the efficient pretreatment of lignocellulosic biomass for bioenergy and bio-products production.

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“…On the other hand, ATPSs can be applied for the characterization of the surface properties for biomolecules such as amino acids, peptides, protein, liposomes, and cells [14][15][16]. The mechanism of distribution in ATPSs is complex and not easy to predict, while the hydrophobicity of the target is one of the most important parameters [13]. The surface net hydrophobicity of protein (HFS) is directly related to the distribution ratio [14].…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, the hydrophilic/hydrophobic balance of Indo-Pep was evaluated from the distribution of Indo-Pep in an aqueous two-phase system (ATPS). Two immiscible phases of an ATPS are formed by combining two hydrophilic solutes (polymer/polymer or polymer/salt) in specific concentrations [11][12][13]. An ATPS has been used as a separation and purification medium for biomolecules such as proteins, due to the biocompatible hydrophilic environment.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Hydrophilic and Hydrophobic Balance for the Complex between Indomethacin and Casein Hydrolysate Using an Aqueous Two-Phase System

Oshima

Inada

Baba

2013

SERDJ

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Complexation with casein hydrolysate (Pep) can be used for enhancing the water solubility of indomethacin. In the present study, the hydrophilic/hydrophobic balance of the complex (Indo-Pep) was evaluated using a poly(ethylene glycol)/dextran (PEG/DEX) aqueous two-phase system (ATPS). As the distribution ratio of Indo-Pep decreases with increasing Pep concentration, indomethacin was found to be more hydrophilic by complexation with Pep. The distribution ratio of Indo-Pep reached a minimum value when the pH was 7-8, where the distribution ratio of Pep showed a maximum value. From the results of the distribution ratios of Indo-Pep prepared using different components of Pep, the hydrophilic/hydrophobic balance of Pep seemed to influence the hydrophilic/hydrophobic balance of Indo-Pep.

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Aqueous two-phase systems for protein separation: A perspective

Cited by 415 publications

References 45 publications

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Purification and characterizations of a novel recombinant Bacillus velezensis endoglucanase by aqueous two-phase system

Evaluation of the Hydrophilic and Hydrophobic Balance for the Complex between Indomethacin and Casein Hydrolysate Using an Aqueous Two-Phase System

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