Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum

Mittal, Rochak; Sharma, Ritu; Raghavarao, K.S.M.S.

doi:10.1016/j.biortech.2019.02.044

Cited by 51 publications

(15 citation statements)

References 34 publications

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“…Aqueous two-phase systems (ATPS) provide mild conditions for the partitioning of thermosensitive and water-prone organic molecules. [1][2][3][4] However, at least several of the commonly used phase formers within an ATPS may cause some environmental distress if applied at industrial scale. 5 A better alternative are biodegradable ATPS composed of nontoxic components, such as polyethylene glycol (PEG) and organic salts, for example, citrates or tartrates.…”

Section: Introductionmentioning

confidence: 99%

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Partitioning of water‐soluble vitamins in biodegradable aqueous two‐phase systems: Electrolyte perturbed‐chain statistical associating fluid theory predictions and experimental validation

et al. 2020

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Partition coefficients (K) of vitamins (riboflavin, nicotinic acid, nicotinamide, folic acid, cyanocobalamin) in aqueous two‐phase systems (ATPS) composed by polyethylene glycol (PEG 4000, PEG 6000) and organic salt (sodium citrate and sodium tartrate) at T = 298.15 K and p = 1 bar have been studied. Data on liquid–liquid equilibria of the ATPS considered in this study have been taken from the literature (PEG‐Na3Citrate) or measured in this work (PEG‐Na2Tartrate) for PEG 4000 and PEG 6000 at T = 298.15 K and p = 1 bar. The experimental K values were validated by electrolyte perturbed‐chain‐statistical associating fluid theory predictions. The neutral cyanocobalamin has the highest K values among all studied vitamins at any ATPS studied in this work. This finding contrasted with expectations based on literature data which let assume that charged species have typically the highest K values in the considered ATPS. Thus, besides the typically strong charge–charge interactions especially specific forces (e.g., hydrogen bonding) explains the strong PEG‐cyanocobalamin interaction resulting in the high K values.

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Section: Introductionmentioning

confidence: 99%

“…Aqueous two‐phase systems (ATPS) provide mild conditions for the partitioning of thermosensitive and water‐prone organic molecules 1‐4 . However, at least several of the commonly used phase formers within an ATPS may cause some environmental distress if applied at industrial scale 5 .…”

Section: Introductionmentioning

confidence: 99%

Partitioning of water‐soluble vitamins in biodegradable aqueous two‐phase systems: Electrolyte perturbed‐chain statistical associating fluid theory predictions and experimental validation

et al. 2020

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“…These reagents have been used for solubilization and/or pre‐precipitation of algal proteins prior to further isolation and fractionation of other compounds of interest, e.g., phenolics or polysaccharides. The use of polyethylene glycol‐1450 in potassium phosphate (pH 6.0) in combination with pre‐precipitation with ammonium sulfate was reported to efficiently extract R‐phycoerythrin (R‐PE) from Gelidium pusillum resulting in a 72% yield [32]. This process, i.e., combined precipitation and two‐phase separation proved highly beneficial in achieving high purity protein samples, such as R‐PE, which possesses various bioactive properties [32].…”

Section: Extraction Of Proteins From Macroalgaementioning

confidence: 99%

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

et al. 2020

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Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed Seaweed (macroalgae) is considered as a sustainable bioresource rich in high-quality nutrients such as protein. Seaweed protein can be used as an alternative to other protein sources. Furthermore, these proteins are natural reservoirs of bioactive peptides (BAPs) associated with various health benefits such as antioxidant, antihypertensive, and antidiabetic activities. However, seaweed-derived BAPs remain underexploited due to challenges that arise during protein extraction from algal biomass. Coupled with this, limited proteomic information exists regarding certain seaweed species. This review highlights the current state of the art of seaweed protein extraction techniques, e.g., liquid, ultrasound, microwave, pulsed electric field, and high hydrostatic pressure assisted extraction. The review also focuses on the enzymatic hydrolysis of seaweed proteins and characterization of the resultant hydrolysates/peptides using electrophoretic and chromatographic techniques. This includes reference to methods employed for separation, fractionation, and purification of seaweed BAPs, as well as the methodologies used for identification, e.g., analysis by mass spectrometry. Furthermore, a bioinformatics or in silico approach to aid discovery of seaweed BAPs is discussed herein. Based on the information available to date, it is suggested that further research is required in this area for the development of seaweed BAPs for nutraceutical applications.

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“…The precipitation process introduced in this experiment (i.e., precipitation by 70% ammonium sulphate ((NH 4 ) 2 SO 4 )) was to pre-purify the crude algae feedstock. The optimised conditions in the integrated LBS with precipitation were the use of PEG 1450 and potassium phosphate, TLL of 12.26% (w/w), V R of 0.29 and pH 6 [45].…”

Section: Application Of Liquid Biphasic System For the Extraction Of mentioning

confidence: 99%

Liquid Biphasic Systems for Oil-Rich Algae Bioproducts Processing

et al. 2019

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Oleaginous algae are nowadays of significance for industrial biotechnology applications and for the welfare of society. Tremendous efforts have been put into the development of economically feasible and effective downstream processing techniques in algae research. Currently, Liquid Biphasic Systems (LBSs) are receiving much attention from academia and industry for their potential as green and effective downstream processing methods. This article serves to review the applications of LBSs (LBS and Liquid Biphasic Flotation System (LBFS)) in the separation, recovery and purification of algae products, as well as their basic working principles. Moreover, cell disruptive technologies incorporated into LBSs in algae research are reported. This review provides insights into the downstream processing in algae industrial biotechnology which could be beneficial for algae biorefinement.

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Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum

Cited by 51 publications

References 34 publications

Partitioning of water‐soluble vitamins in biodegradable aqueous two‐phase systems: Electrolyte perturbed‐chain statistical associating fluid theory predictions and experimental validation

Partitioning of water‐soluble vitamins in biodegradable aqueous two‐phase systems: Electrolyte perturbed‐chain statistical associating fluid theory predictions and experimental validation

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

Liquid Biphasic Systems for Oil-Rich Algae Bioproducts Processing

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