Liquid Biphasic Systems for Oil-Rich Algae Bioproducts Processing

Abstract

Oleaginous algae are nowadays of significance for industrial biotechnology applications and for the welfare of society. Tremendous efforts have been put into the development of economically feasible and effective downstream processing techniques in algae research. Currently, Liquid Biphasic Systems (LBSs) are receiving much attention from academia and industry for their potential as green and effective downstream processing methods. This article serves to review the applications of LBSs (LBS and Liquid Biphasic Flotation System (LBFS)) in the separation, recovery and purification of algae products, as well as their basic working principles. Moreover, cell disruptive technologies incorporated into LBSs in algae research are reported. This review provides insights into the downstream processing in algae industrial biotechnology which could be beneficial for algae biorefinement.

Downstream processing of virus‐like particles with aqueous two‐phase systems: Applications and challenges

“…Aqueous two‐phase extraction (ATPE), which based on ATPSs, is a promising downstream processing technology in biotechnological industry and it has developed as new prospects in biomedical applications [13, 33, 34]. ATPSs have been long explored as a unique and efficient extraction pipeline, which offers a single‐step procedure for the separation, recovery, purification, and enrichment of biomolecules such as enzymes, cells, particles [35], peptides, proteins [36–41], antibodies [42, 43], pigments [44–47], algae‐derived bioproducts [48], and natural products [49–53].…”

“…ATPSs have been long explored as a unique and efficient extraction pipeline, which offers a single-step procedure for the separation, recovery, purification, and enrichment of biomolecules such as enzymes, cells, particles [35], peptides, proteins [36][37][38][39][40][41], antibodies [42,43], pigments [44][45][46][47], algae-derived bioproducts [48], and natural products [49][50][51][52][53].…”

Algae-derived hydrocolloids in foods: applications and health-related issues

“…Red and brown algae can be employed to produce hydrocolloids (e.g. agar, alginate, and carrageenan) as thickening and gelling agents [ 20 , 21 ]. Notably, algae are mostly autotrophs, a feature allowing simple and economical cultivation for commercial production of food additives, cosmetics, animal feed additives, pigments, polysaccharides, fatty acids, and biomass [ 16 ].…”

Assuaging Microalgal Harvesting Woes via Attached Growth: A Critical Review to Produce Sustainable Microalgal Feedstock

“…In fact, the harvesting process will reduce the possibility of the water recycling to be used as the growth medium and its direct release into the environment [65,66]. Moreover, Leong et al [71] proposed in their studies that a single processing unit involving the harvesting, cell disruption, and extraction of microalgae products based on Liquid Biphasic systems (LBs) are quite promising, especially for the microalgae bio-refinement and improving the techno-economic feasibility of microalgae cultivation. Liquid Biphasic systems conduct a gentle working environment for the target microalgae products' recovery and can extract the products of interest without damaging the microalgae cell and chemical elements [72,73].…”