Aqueous Rosa damascena extract: Antibacterial activity and its role of adhesion to human epithelial cells in vitro

Abstract

The current study aimed to investigate the inhibitory activity of aqueous extracts of different plant parts of Rosa damascena, represented by the whole rose, petals, and calyces, against clinical isolates of Staphylococcus spp., Escherichia coli, and Klebsiella pneumoniae, and the inhibition of the bacterial cell. The isolates were obtained from the advanced microbiology laboratory for postgraduate studies in the Department of Biology, Faculty of Education for Girls. They were isolated from urinary tract infections, which were subsequently subjected to diagnosis by the Vitek-2 compact system to confirm the type of bacteria as well as their sensitivity to antibiotics. The results obtained included Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus lentus, Staphylococcus saprophyticus, E. coli, and K. pneumoniae. A test was conducted to investigate the microbiological inhibitory activity of aqueous plant extracts of the whole rose, petals, and calyces using the well diffusion method and three concentrations of each aqueous extract (25, 50, and 100 mg/ml). The results showed the inhibitory ability of all concentrations of the different extracts toward Staphylococcus spp., and E. coli and K. pneumoniae bacteria were not affected by the different concentrations of the plant extract. The concentration of (100 mg/ml) for the aqueous extract was the most efficient in inhibiting growth compared to the other concentrations. The synergistic effect of three antibiotics was examined (Amoxicillin-clavulanate 10/20 μg, Piperacillin 100 μg, Trimethoprimsulfamethoxazole 23.75/1.25 μg) and for all concentrations of the aqueous plant extract was investigated in both E. coli and K. pneumoniae, as it found a synergistic action between some of the antibiotics and extracts towards inhibiting the growth of the two bacterial isolates Resistance to the plant extract alone. Bacterial isolates showed a significant decrease in the rate of adhesion to epithelial cells isolated from urine samples of healthy women in the presence of the aqueous extract of whole rose, petals, and calyces at their three concentrations compared with the control treatment.

The In Vitro Assessment of Antibacterial and Antioxidant Efficacy in Rosa damascena and Hypericum perforatum Extracts against Pathogenic Strains in the Interplay of Dental Caries, Oral Health, and Food Microbiota

“…Performing a bibliometric overview on the study of R. damascene extracts against pathogenic bacteria, we notice that S. aureus is the most studied bacterium, against which it is confirmed that various types of extracts, including aqueous, ethanolic, and methanolic extracts, show strong antimicrobial activity [ 102 , 103 ]. This is the reason that we also incorporated in S. aureus our study.…”

“…Second, the presence of unknown substances in the extracts poses a challenge in understanding potential synergistic or antagonistic effects among the compounds. Thus, we emphasize the need for caution in interpreting the overall impact of the extracts due to these uncertainties [ 102 ]. Additionally, the strain-specific responses observed in the study’s time-kill curves highlight the complexity of microbial interactions, indicating that certain strains may exhibit higher resistance or require longer exposure times [ 114 ].…”