Aqueous fraction from Cuscuta japonica seed suppresses melanin synthesis through inhibition of the p38 mitogen-activated protein kinase signaling pathway in B16F10 cells

Jang, Ji Yeon; Kim, Ha‐Neui; Kim, Yu Ri; Choi, Yung Hyun; Kim, Byung Woo; Shin, Hwa Kyoung; Choi, Byung Tae

doi:10.1016/j.jep.2012.02.043

Cited by 30 publications

(20 citation statements)

References 23 publications

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“…However, quercetin and eriodictyol, which have substituents on the C ring that differ from that of luteolin, showed no extracellular melanogenesis inhibitory activity. In addition, apigenin, chrysin, activity [21][22][23]. In particular, the determination of the downregulation of the expression of activated p38 and MITF is commonly used to investigate the mechanism by which compounds inhibit melanogenesis [21,24].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…In addition, apigenin, chrysin, activity [21][22][23]. In particular, the determination of the downregulation of the expression of activated p38 and MITF is commonly used to investigate the mechanism by which compounds inhibit melanogenesis [21,24]. In the case of ugonin K, it is noteworthy that previous studies have reported that p38 MAPK stimulation also plays an important role in osteoblastic differentiation [25].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

See 1 more Smart Citation

Extracellular melanogenesis inhibitory activity and the structure-activity relationships of ugonins from Helminthostachys zeylanica roots

et al. 2015

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Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

Extracellular melanogenesis inhibitory activity and the structure-activity relationships of ugonins from Helminthostachys zeylanica roots

et al. 2015

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“…It has been demonstrated that numerous N -acetyl-peptides exhibit potent biological activity, such as being chemoattractants or exerting an antifibrosis effect by binding to surface receptors [35, 44]. It is unclear whether Ac-P4 can competitively bind to membrane receptors such as melanocortin 1 receptor (MCR-1) and thereby repress alpha-melanocyte stimulating hormone- ( α -MSH-) induced melanogenesis.…”

Section: Resultsmentioning

confidence: 99%

“…Cellular melanin content was determined as described previously [35]. Briefly, 1 × 10 5 B16F10 cells were seeded in 24-well plates and cultured at 37°C and 5% CO 2 for 48 h. Subsequently, the cells were treated with 100 nM α -MSH for 24 h, followed by various concentrations (0, 0.1, 0.5, and 1 mg/mL) of Ac-P4 or kojic acid for 24 h. The cells were washed twice with PBS and cell pellets were then dissolved in 100 μ L of 1 N NaOH in 10% DMSO, for 1 h at 80°C.…”

Section: Methodsmentioning

confidence: 99%

Kinetics of Mushroom Tyrosinase and Melanogenesis Inhibition byN-Acetyl-pentapeptides

Lien

Chen

Lai

et al. 2014

The Scientific World Journal

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We investigated the kinetics of 4N-acetyl-pentapeptides, Ac-P1, Ac-P2, Ac-P3, and Ac-P4, regarding inhibition of mushroom tyrosinase activity. The peptides sequences of Ac-P1, Ac-P2, Ac-P3, and Ac-P4 were Ac-RSRFK, Ac-KSRFR, Ac-KSSFR, and Ac-RSRFS, respectively. The 4N-acetyl-pentapeptides were able to reduce the oxidation of l-DOPA by tyrosinase in a dose-dependent manner. Of the 4N-acetyl-pentapeptides, only Ac-P4 exhibited lag time (80 s) at a concentration of 0.5 mg/mL. The tyrosinase inhibitory effects of Ac-P4 (IC50 0.29 mg/mL) were more effective than those of Ac-P1, Ac-P2, and Ac-P3, in which IC50s were 0.75 mg/mL, 0.78 mg/mL, and 0.81 mg/mL, respectively. Kinetic analysis demonstrated that all 4N-acetyl-pentapeptides were mixed-type tyrosinase inhibitors. Furthermore, 0.1 mg/mL of Ac-P4 exhibited significant melanogenesis inhibition on B16F10 melanoma cells and was more effective than kojic acid. The melanogenesis inhibition of Ac-P4 was dose-dependent and did not induce any cytotoxicity on B16F10 melanoma cells.

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“…[18] Recent studies have reported that the Chinese herbal formula of San-bai-tang and the aqueous fraction from the Cuscuta japonica seed inhibit melanogenesis by downregulating the expression of MITF and melanogenic enzymes through the suppression of p38 MAPK [19,20]. Thus, the inhibition of the p38 MAPK cascade may be critical to downregulate melanogenesis.…”

Section: Discussionmentioning

confidence: 99%

Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells

Kim

Yim

et al. 2013

BMC Complement Altern Med

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BackgroundSsanghwa-tang (SHT) is a widely used medication for the treatment of fatigue, pain, inflammation, hypothermia, erectile dysfunction, cancer, and osteoporosis in Asia, however, role of SHT on the melanin synthesis has not been checked previously. Thus, the present study was designed to determine the effect of SHT on α-melanocyte stimulating hormone (α-MSH)-induced melanogensis and its mechanisms of action in murine B16F10 melanoma cells.MethodCellular melanin content and tyrosinase activity in murine B16F10 melanoma cells were determined after α-MSH stimulation with or without pre-treatment of SHT at the concentration of 250 and 500 μg/ml. Expression level of tyrosinase, tyrosinase-related protein 1 (TRP-1), TRP-2, microphthalmia-associated transcription factor (MITF), and activation of c-AMP-dependent protein kinase (PKA), c-AMP-related element binding protein (CREB), and mitogen-activated protein kinases (MAPKs) were examined by Western blot analysis.ResultsSHT significantly inhibited α-MSH-induced melanin synthesis and tyrosinase activity, and also decreased α-MSH-induced expression of MITF, tyrosinase, and TRP-1. In addition, SHT remarkably suppressed tyrosinase, CRE, and MITF luciferase reporter activity in a resting state as well as in α-MSH-stimulating condition. Phosphorylation of p38 MAPK by α-MSH stimulation was efficiently blocked by SHT pre-treatment. Moreover, SHT as an herbal cocktail showed synergistic anti-melanogenic effect compared with that of each single constituent herb.ConclusionSHT efficiently inhibited c-AMP-induced melanin synthesis in B16F10 cells via suppression of PKA and p38 MAPK signaling pathways and subsequently decreased the level of CREB phosphorylation, MITF, and melanogenic enzymes. These results indicate that SHT may be useful as herbal medicine for treating hyperpigmentation and cosmetics as a skin-whitening agent.

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Aqueous fraction from Cuscuta japonica seed suppresses melanin synthesis through inhibition of the p38 mitogen-activated protein kinase signaling pathway in B16F10 cells

Cited by 30 publications

References 23 publications

Extracellular melanogenesis inhibitory activity and the structure-activity relationships of ugonins from Helminthostachys zeylanica roots

Extracellular melanogenesis inhibitory activity and the structure-activity relationships of ugonins from Helminthostachys zeylanica roots

Kinetics of Mushroom Tyrosinase and Melanogenesis Inhibition byN-Acetyl-pentapeptides

Ssanghwa-tang, an oriental herbal cocktail, exerts anti-melanogenic activity by suppression of the p38 MAPK and PKA signaling pathways in B16F10 cells

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