Aqueous Flare and Cell Meter in Iridocyclitis

Ohara, Kunitoshi; Ōkubo, Akira; Miyazawa, Atsuko; Sekiya, Hiroshi

doi:10.1016/0002-9394(88)90889-6

Cited by 17 publications

(4 citation statements)

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“…Electron microscopic studies of iris vessels in PEX revealed deposi tion o f PEX material in the vessel walls with consecutive disorganization of the normal ves sel structure, loss of endothelial and adventitial cells, and thinning and fenestration of the endo thelial lining [6, 39^15], Using immunoelectron microscopic staining for endogenous albu min and immunoglobulin G, Schlotzer-Schrchardt et al [46] could localize the primary sites of protein leakage in the iris root and the ante rior ciliary body. With the LFCM, alterations of the BAB have been analyzed and quantified in a variety of diseases, including intraocular in flammation [15,[28][29][30], postoperative ocular changes [15,[47][48][49], diabetic retinopathy [23 25], retinal vein occlusion [26,27] as well as malignant melanoma [50][51][52]. The LFCM is al so useful in assessing less extensive changes of the BAB such as in PEX eyes.…”

Section: Discussionmentioning

confidence: 99%

“…Both for the tyndallometric and for the biochemical studies, eyes with additional ocular alterations that may lead to impairment o f the BAB, such as diabetic reti nopathy [23][24][25], retinal vein occlusion [26,27], uveitis [28][29][30] or previous intraocular surgery, were excluded. The tenets o f the World Medical Association Declara tion of Helsinki were followed, and local Ethical Com mittee approval was granted.…”

Section: Ba B Breakdown Following Intraocular Surgery In Pexmentioning

confidence: 99%

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The Blood-Aqueous Barrier in Eyes with Pseudoexf oliation Syndrome

et al. 1995

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Eyes with pseudoexfoliation syndrome (PEX) frequently show clinical signs of impairment of the blood-aqueous barrier. Herein we give an overview of recent studies that analyzed the blood-aqueous barrier in eyes with PEX. Methods: The authors review and summarize recent studies including quantification of aqueous flare in eyes with PEX using the laser flare cell meter (LFCM; Kowa FC-1000) in comparison with normal eyes and eyes with primary open-angle glaucoma (POAG), quantification of aqueous flare in eyes with PEX with and without secondary open-angle glaucoma (SOAG), and quantitative biochemical determination of total aqueous protein concentration in PEX eyes. In addition, studies of noninvasive quantification of the blood-aqueous barrier breakdown following trabeculectomy and following phacoemulsification with intraocular lens implantation in eyes with and without PEX are reviewed. Results: In eyes with manifest PEX, both aqueous flare and aqueous protein concentration were significantly increased in comparison with normal control eyes and eyes with POAG. Flare values in PEX eyes with SOAG were not significantly different from flare values in PEX eyes without SOAG. Following trabeculectomy as well as following cataract surgery, breakdown of the blood-aqueous barrier as determined by quantification of aqueous flare was significantly higher in eyes with PEX than in eyes without PEX. Conclusions: Impairment of the blood-aqueous barrier with increase in aqueous protein concentration is a feature of PEX and may be quantified both by flare measurement and by biochemical protein determination. The extensive blood-aqueous barrier breakdown in eyes with PEX following intraocular surgery is an important risk factor for early or late postoperative complications. The alterations of the blood-aqueous barrier should be considered in the medical and surgical treatment of eyes with PEX.

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Section: Discussionmentioning

confidence: 99%

Section: Ba B Breakdown Following Intraocular Surgery In Pexmentioning

confidence: 99%

The Blood-Aqueous Barrier in Eyes with Pseudoexf oliation Syndrome

et al. 1995

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“…These studies are similar to early studies on laser flare photometry, which also demonstrated linear response to in vitro BSA concentrations 5 and general correlation with observed qualitative flare levels. 5,18,[23][24][25] As in previous studies with laser flare photometry (see Fig. 3 of the study of Ladas et al 5 ) we saw substantial range of flare values for subjects within one clinical grade, and saw substantial overlap in grading of individuals with particular flare values.…”

Section: Discussionmentioning

confidence: 55%

A Rayleigh Scatter-Based Ocular Flare Analysis Meter for Flare Photometry of the Anterior Chamber

Lam

Axtelle

Rath

et al. 2015

Trans. Vis. Sci. Tech.

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Purpose: Existing flare photometers are based on the Tyndall effect, which requires sophisticated laser photometry. The ocular flare analysis meter (OFAM) is a nonlaser photometer that uses quantitative Rayleigh scatter and absorption from visible light to compute a flare value. This study is designed to correlate OFAM measurements with qualitative measurements of flare in vitro and in vivo.Methods: Following validation of the device on artificial anterior chambers containing known protein concentrations, flare readings were obtained from 90 subjects (46 with and 44 without uveitis) in one eye. Subjects were graded by the Standardization of Uveitis Nomenclature (SUN) working group flare scoring system and received the OFAM flare measurements.Results: The OFAM showed linear response in vitro to protein concentrations ranging from 0 to 0.5 mg/ml. In clinical use in subjects ranging from SUN flare scores of 0þ to 2þ, OFAM showed statistically significant measurement accuracy (P ¼ 0.0008 of flare 0 versus flare 2; P ¼ 0.031 of flare 0 versus flare 1). Distinction of SUN scores 1 and 2 was borderline significant (P ¼ 0.057). Conclusion:The OFAM photometry correlates with the standard SUN scoring system. This method may provide an objective method to diagnosis and monitor uveitis. Further longitudinal studies are warranted.Translational Relevance: Currently, ocular flare is assessed qualitatively in most clinical settings. The existing methodology uses only Tyndall effect to measure flare. The OFAM uses an alternate, nonlaser means for measurement of anterior chamber flare by measure of Raleigh scatter. This pilot clinical study suggests that the OFAM device may be useful in measurement of uveitis activity.

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“…The apparatus and technique have been described previously [21,22,23]. The sensitivity and reproducibility of the method have been confirmed by a number of groups in a series of studies [8,19,22,23,25,26,27]. In the present study, aqueous flare was quantified by three skilled examiners at defined postoperative intervals (10 days, 6 weeks, then every 3 months until 1 year postoperatively).…”

Section: Methodsmentioning

confidence: 71%

Blood-aqueous barrier breakdown after penetrating keratoplasty with simultaneous extracapsular cataract extraction and posterior chamber lens implantation

Nguyen¹,

Langenbucher²,

Seitz³

et al. 2001

Graefe's Arch Clin Exp Ophthalmol

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Our results indicate that triple procedure causes a more extensive and longer-lasting breakdown of the blood-aqueous barrier than PK only. Quantification of aqueous flare with the laser flare-cell meter is useful in the postoperative follow-up after triple procedure. Further studies are required to investigate the clinical relevance of BAB breakdown on endothelial cell count and the incidence of subsequent immunological graft rejection.

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Aqueous Flare and Cell Meter in Iridocyclitis

Cited by 17 publications

References 1 publication

The Blood-Aqueous Barrier in Eyes with Pseudoexf oliation Syndrome

The Blood-Aqueous Barrier in Eyes with Pseudoexf oliation Syndrome

A Rayleigh Scatter-Based Ocular Flare Analysis Meter for Flare Photometry of the Anterior Chamber

Blood-aqueous barrier breakdown after penetrating keratoplasty with simultaneous extracapsular cataract extraction and posterior chamber lens implantation

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