Aqueous extract of the Helianthus annuus seed alleviates asthmatic symptoms in vivo

Abstract

Molecular inflammation is a pivotal process in various degenerative immune diseases, including asthma and atopic dermatitis. In this study, we examined the effects of Helianthus annuus seed (HAS) aqueous extract on an in vivo anti-asthmatic model. Ovalbumin-induced mice were orally administered the aqueous extract of Helianthus annuus seeds, and their lungs were assessed by hematoxylin and eosin staining. Moreover, the expression levels of IL-4/IL-13 cytokines and IgE were determined. HAS extract induced a decrease in CD4 + cell number, IL-4/IL-13 expression, and IgE secretion levels in the lungs. Our findings collectively suggest that the HAS extract has considerable potential in reducing the asthma-like symptoms induced by a mouse ovalbumin challenge model. However, further isolation and purification of the extract is required to determine the specific factor(s) responsible for its anti-asthmatic activity.Abbreviations: HAS, Helianthus annuus seeds; DPPH, 1,1diphenyl-2-picrylhydrazyl; Fe(III)-TPTZ, ferric tripyridyltriazine; EDTA, ethylenediamine tetraacetic acid; FRAP, ferric ion-reducing antioxidant power; MMP, matrix metalloproteinase; IL, interleukin

Amelioration of asthmatic inflammation by an aqueous extract of Spinacia oleracea Linn

“…To further evaluate whether the molecular markers related to asthma decrease during SoL treatment, IL-4, IL-13 and IgE levels in serum were measured using ELISA (19,20). The IL-4 content was 91.5±20.9 pg/ml in the control group and 285.1±38.9 pg/ml in the ovalbumin-challenged group.…”

Anti-asthmatic activities in mycelial extract and culture filtrate of Cordyceps sphecocephala J201

“…Briefly, a 0.4 mM solution of DPPH in 50% ethanol was prepared and 200 μl of this solution was added to 2 μl of a differential concentration of the samples. The mixture was shaken vigorously and allowed to stand at room temperature for 30 min (28). Then, the absorbance was measured at 517 nm in a multilabel counter (Victor3, PerkinElmer, Waltham, MA, USA) (13).…”

“…Then, the absorbance was measured at 517 nm in a multilabel counter (Victor3, PerkinElmer, Waltham, MA, USA) (13). Ferric reducing antioxidant power (FRAP) assay was also carried out as describe elsewhere (28), with a slight modification. Briefly, the FRAP reagent contained 2.5 ml of 10 mM tripyridyltriazine (FRAP) solution in 10 mM HCl plus 2.5 ml of 20 mM FeCl 3 and 25 ml of 0.3 M acetate buffer, pH 3.6, was freshly prepared.…”

A Derivative ofL-Allo Threonine Alleviates 2,4-Dinitrofluorobenzene-Induced Atopic Dermatitis Indications

“…Measurement of the results of immunohistochemical analysis were done by comparing the staining of immune positive cells to IL-17E (Santa Cruz Biotechnology, Santa Cruz, CA) or histamine (Santa Cruz Biotechnology). 16,17) Positive cell numbers were calculated as values per mm of basement membrane.…”