Aqueous extract of Taxus chinensis var. mairei regulates the Hippo-YAP pathway and promotes apoptosis of non-small cell lung cancer via ATF3 in vivo and in vitro

Zhang, Gaochenxi; Dai, Shuguang; Chen, Yiyi; Wang, Haibin; Chen, Ting; Shu, Qijin; Chen, Shuyi; Shou, Liu‐Mei; Cai, Xiaona

doi:10.1016/j.biopha.2021.111506

Cited by 12 publications

(8 citation statements)

References 28 publications

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“…The preparation of AETW was based on the procedure detailed in a previous study ( Zhang et al, 2021a ). A total of 12 g of TW was put into a clean porcelain jar and soaked overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Until the extraction of Taxol with a broad-spectrum anti-tumor effect from Taxus L. plant in the 1970s, TW has been widely concerned for its pharmacological value ( Wani et al, 1971 ). Currently, abundant evidence has confirmed that the extract of TW is able to inhibit proliferation, induce apoptosis, strengthen immunity, and reverse drug resistance in lung cancer cells ( Jiang et al, 2016 ; Zhang et al, 2021a ). However, the function and the underlying mechanism of TW in preventing or reversing cancer metastasis have been rarely reported.…”

Section: Introductionmentioning

confidence: 99%

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Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway

Sun

Ding

Luo³

et al. 2021

Front. Pharmacol.

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As one of the most common neoplasms globally, lung cancer (LC) is the leading cause of cancer-related mortality. Recurrence and metastasis negatively influencing therapeutic efficacy and overall survival demand new strategies in LC treatment. The advantages of TCM are increasingly highlighted. In this study, we obtained the major chemical components and their ratios in the aqueous extract of Taxus wallichiana var. chinensis (Pilg.) Florin (AETW) by UPLC-Q/TOF-MS/MS detection. The CCK-8 assay revealed that AETW could selectively inhibit the growth of A549 and HCC827 cells in a dose-dependent manner with little effect on normal human lung cells. Moreover, both in vitro and in vivo experiments showed that AETW was able to suppress the capacities of cell migration and invasion and downregulate the EMT and the JAK/STAT3 signaling pathway. To further probe into the molecular mechanism, the overexpression of STAT3 was performed into LC cells with AETW treatment, which counteracted the inhibitory effect on malignant behaviors of A549 and HCC827 cells with the decline in the expressions of p-JAK and p-STAT3. Taken together, we propose that AETW may inhibit the proliferation and metastasis by inactivating the JAK/STAT3 axis.

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“…The preparation of AETW was based on the procedure detailed in a previous study ( Zhang et al, 2021a ). A total of 12 g of TW was put into a clean porcelain jar and soaked overnight.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway

Sun

Ding

Luo³

et al. 2021

Front. Pharmacol.

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“…Taxus species are also traditional medicinal plants, the shoots and leaves have been used to treat kidney disease and rheumatism 14 . Multiple studies showed that Taxus species have anticancer activities, such as treating pancreatic cancer with water decoctions of Taxus cuspidate 15 and treating non-small cell lung cancer with aqueous extracts 16 . Therefore, itʼs important to explore the new anti-tumour endophytic fungi from these two medicinal plants.…”

Section: Panax Notoginseng Has Been Widely Used In Traditionalmentioning

confidence: 99%

Acute Anti-Cancer Activity of Crude Extracts from two Endophytic Fungi Chaetomium cochliodes and Penicillium Sp. in Cancer Cell Lines and Mice

Xiao¹,

Ma²,

Cai³

et al. 2022

International J. of Pharmacology

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Background and Objective: The discovery of novel natural lead compounds is the key issue in searching for new anti-tumour drugs.Endophytic fungi have been proved to be a promising source for new bioactive compounds with potential application in medicine. This study aimed to investigate the activity of antiproliferation, cell cycle arrest, induction of apoptosis and anti-tumour growth of the crude extracts from the two endophytic fungi, Chaetomium cochliodes and Penicillium sp. Materials and Methods: The crude extracts of the fungi Chaetomium cochliodes and Penicillium sp., were obtained by enrichment and extraction in vitro. The proliferation, cell cycle and apoptosis were evaluated the killing effects of tumour cells and cytotoxicity in Huh7 and MGC-803 cell lines. The effect of the extract in vivo was studied in a subcutaneously implanted tumour model in C57BL/6 mice. Results: The cancer cell survival rate was almost less than 10% after the treatment of the crude extracts of Chaetomium cochliodes and Penicillium sp., by using a cell proliferation assay. Through the apoptosis assay, the crude extracts from Chaetomium cochliodes and Penicillium sp., significantly increased the number of apoptosis compared with the control group. The crude extract from Chaetomium cochliodes mainly induced G2/M phase arrest of MGC-803 cells, which might be contributing to the apoptosis. Importantly, these two crude extracts significantly reduced the volume and size of subcutaneous transplanted tumours after treatment in transplanted tumour models. Conclusion: The present study demonstrated that the extracts obtained from Chaetomium cochliodes and Penicillium sp., are promising materials for tumour treatment. These results expand the medicinal value of Panax notoginseng and Taxus media and provide a new direction for its clinical application.

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“…YAP and TAZ have been implicated in many in the periodontium, including myofibroblast differentiation, osteogenesis, and oral mechanobiological carcinogenesis processes. Addressing these Hippo components pharmacologically is not a new concept, as their role in many different human cancers makes them a promising target to slow down disease progression or hopefully to suppresses features of malignant transformed tumor cells [164,360,361]. There exist different mechanisms of how to prevent YAP nuclear entry and thus induction of proliferation and EMT.…”

Section: May the Force Be With You: Mt And Its Implications For Periodontal Regenerationmentioning

confidence: 99%

From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues

et al. 2021

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Among oral tissues, the periodontium is permanently subjected to mechanical forces resulting from chewing, mastication, or orthodontic appliances. Molecularly, these movements induce a series of subsequent signaling processes, which are embedded in the biological concept of cellular mechanotransduction (MT). Cell and tissue structures, ranging from the extracellular matrix (ECM) to the plasma membrane, the cytosol and the nucleus, are involved in MT. Dysregulation of the diverse, fine-tuned interaction of molecular players responsible for transmitting biophysical environmental information into the cell’s inner milieu can lead to and promote serious diseases, such as periodontitis or oral squamous cell carcinoma (OSCC). Therefore, periodontal integrity and regeneration is highly dependent on the proper integration and regulation of mechanobiological signals in the context of cell behavior. Recent experimental findings have increased the understanding of classical cellular mechanosensing mechanisms by both integrating exogenic factors such as bacterial gingipain proteases and newly discovered cell-inherent functions of mechanoresponsive co-transcriptional regulators such as the Yes-associated protein 1 (YAP1) or the nuclear cytoskeleton. Regarding periodontal MT research, this review offers insights into the current trends and open aspects. Concerning oral regenerative medicine or weakening of periodontal tissue diseases, perspectives on future applications of mechanobiological principles are discussed.

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Aqueous extract of Taxus chinensis var. mairei regulates the Hippo-YAP pathway and promotes apoptosis of non-small cell lung cancer via ATF3 in vivo and in vitro

Cited by 12 publications

References 28 publications

Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway

Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway

Acute Anti-Cancer Activity of Crude Extracts from two Endophytic Fungi Chaetomium cochliodes and Penicillium Sp. in Cancer Cell Lines and Mice

From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues

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