Taxus wallichiana var. chinensis (Pilg.) Florin Aqueous Extract Suppresses the Proliferation and Metastasis in Lung Carcinoma via JAK/STAT3 Signaling Pathway

Abstract

As one of the most common neoplasms globally, lung cancer (LC) is the leading cause of cancer-related mortality. Recurrence and metastasis negatively influencing therapeutic efficacy and overall survival demand new strategies in LC treatment. The advantages of TCM are increasingly highlighted. In this study, we obtained the major chemical components and their ratios in the aqueous extract of Taxus wallichiana var. chinensis (Pilg.) Florin (AETW) by UPLC-Q/TOF-MS/MS detection. The CCK-8 assay revealed that AETW could selectively inhibit the growth of A549 and HCC827 cells in a dose-dependent manner with little effect on normal human lung cells. Moreover, both in vitro and in vivo experiments showed that AETW was able to suppress the capacities of cell migration and invasion and downregulate the EMT and the JAK/STAT3 signaling pathway. To further probe into the molecular mechanism, the overexpression of STAT3 was performed into LC cells with AETW treatment, which counteracted the inhibitory effect on malignant behaviors of A549 and HCC827 cells with the decline in the expressions of p-JAK and p-STAT3. Taken together, we propose that AETW may inhibit the proliferation and metastasis by inactivating the JAK/STAT3 axis.

Integrating Network Pharmacology and Experimental Validation to Explore the Effects and Mechanisms of Qinghao Biejia Decoction and Its Active Compound Artemisinin B Against Non-Small-Cell Lung Cancer

“…The UPLC and MS conditions have been developed and validated based on our previously reported protocols with some modifications. 15 A Waters Acquity UPLC system (MA, USA) and a CORTECS ® UPLC ® C18 column (2.1 mm × 100 mm, 1.7 μm) were applied to the chromatographic separation of QBD. The mobile phase was composed of 0.1% formic acid water solution (mobile phase A) and acetonitrile (mobile phase B).…”

“…The UPLC and MS conditions have been developed and validated based on our previously reported protocols with some modifications. 15 A Waters Acquity UPLC system (MA, USA) and a CORTECS ® UPLC ® C18 column (2.1 mm × A Waters Xevo QTS MS/MS (MA, USA) with an electron spray ionization (ESI) source was used for MS detection in both positive-and negative-ion modes. The optimized mass parameters were set as follows: full scan range: (m/z 100-1500), argon as collision gas, and nitrogen as nebulizer and heater gases (500 L/h).…”

Advancements in the Cultivation, Active Components, and Pharmacological Activities of Taxus mairei

“…Additionally, the CCK-8 assay showed that TAE has a selective inhibitory effect on the growth of non-small cell lung cancer A549 and HCC827 cells and is dose-dependent, with minimal impact on everyday human lung cells. Its mechanism may be related to inhibiting tumor cell proliferation and metastasis by inactivating the JAK/STAT3 axis [ 77 , 78 ]. Research also indicated that taxane compounds extracted from T. mairei exhibit a specific inhibitory effect on the proliferation of A549 non-small cell lung cancer cells (IC50 between 26–167 μg/mL).…”

Cancer treatment: from traditional Chinese herbal medicine to the liposome delivery system

“…Many studies have confirmed that PTX inhibits the growth of a variety of tumors [55]. The main mechanism of action for PTX is to cause abnormal arrangement of microtubules and the production of microtubule stellates during cell proliferation to hinder cell amplification, inhibit tumor growth and proliferation, induce apoptosis, and enhance innate immunity [56].…”