Aqueous-ethanolic extract of morel mushroom mycelium Morchella esculenta, protects cisplatin and gentamicin induced nephrotoxicity in mice

Nitha, B; Janardhanan, K. K.

doi:10.1016/j.fct.2008.07.007

Cited by 85 publications

(37 citation statements)

References 50 publications

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“…This reaction has a 10000-fold faster rate than spontaneous dismutation. 49) This reduction in SOD and CAT activities after GM injection has been previously recorded 50,51) respectively, suggesting that oxidative stress is one of the causes of GM-induced renal damage. Interestingly, the combined administration of quercetin plus GM to rats reversed all of these alterations.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Quercetin against Gentamicin-Induced Nephrotoxicity in Rats

Abdel-Raheem

Abdelghany

Mohamed

2009

Biological & Pharmaceutical Bulletin

152

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Gentamicin (GM) is an antibiotic widely used in treating severe gram-negative infections. However, its clinical use is limited by its nephrotoxicity. Several lines of evidence indicate that free radicals are important mediators of gentamicin nephrotoxicity. Therefore, the aim of this work was to investigate the possible protective effect of the flavonoid quercetin, an antioxidant, on gentamicin-induced nephrotoxicity. For this purpose, rats were divided into four groups. First group served as a control and injected with the normal saline, second group was injected with quercetin (50 mg/kg/d, per os) for 7 d, third group was injected with gentamicin (80 mg/kg/d, intraperitoneally) for 7 d and the fourth group of animals was injected with quercetin plus gentamicin simultaneously for 7 d. Total protein levels were estimated in 24-h urine samples to assess kidney dysfunction. The rats were sacrificed on the seventh day and kidneys were collected for histopathological studies. Blood urea nitrogen (BUN) and creatinine levels were measured in the blood. Moreover, glutathione (GSH), lipid peroxide (TBARS) levels, superoxide dismutase (SOD) and catalase (CAT) activities were determined in renal tissues. GM-treated rats showed early kidney dysfunction as urinary total protein, BUN and serum creatinine levels were significantly increased. The significant decrease in GSH levels, SOD, CAT activities and increase in TBARS levels, indicated that GMinduced nephrotoxicity was mediated through oxidative stress reactions. Histopathological examination of GM-treated rats revealed degenerative changes in glomeruli and tubules. On the other hand, simultaneous administration of quercetin plus gentamicin protected kidney tissues against nephrotoxic effects of gentamicin as evidenced from amelioration of histopathological changes and normalization of kidney biochemical parameters.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Quercetin against Gentamicin-Induced Nephrotoxicity in Rats

Abdel-Raheem

Abdelghany

Mohamed

2009

Biological & Pharmaceutical Bulletin

152

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“…The decreased SOD activity is not enough to scavenge the superoxide anion produced during the normal metabolic process and cause the initiation and propagation of lipid peroxidation in GM-treated group (Nitha & Janardhanan, 2008). The activities of CAT and GSH-Px also decreased in the GM-treated group, which resulted in the decreased ability of renal to scavenge toxic hydrogen peroxide and lipid peroxidation (Badary et al, 2005;Nitha & Janardhanan, 2008). Therefore, the significant increase in the level of MDA, as marker of lipid peroxidation, was observed in the GM-treated group.…”

Section: N-acetylcysteinementioning

confidence: 99%

“…Group II (GM-treated group) animals were injected i.p. with GM (100 mg/kg body weight) for 8 days (Nitha & Janardhanan, 2008;Thounaojam et al, 2010). Group III and IV animals were administered orally with EMO (250 and 500 mg/kg body weight) 2 h prior to injection i.p.…”

Section: Gentamicin-induced Acute Renal Toxicity In Ratsmentioning

confidence: 99%

Nephroprotective activity ofMacrothelypteris oligophlebiarhizomes ethanol extract

Cai

Han

et al. 2011

Pharmaceutical Biology

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“…These pharmacological approaches have mainly focused on discovering compounds of natural origin (Nagwani & Tripathi, 2010;Nitha & Janardhanan, 2008;Sohn et al, 2009), notably issuing from Traditional Chinese Medicine (TCM) remedies.…”

Section: Introductionmentioning

confidence: 99%

Potential nephroprotective effects of the Chinese herbAngelica sinensisagainst cisplatin tubulotoxicity

Bunel

Antoine

Nortier

et al. 2014

Pharmaceutical Biology

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Context: Acute kidney injury (AKI) is often encountered in patients receiving cisplatin (CisPt), a chemotherapeutic drug that induces numerous toxic side effects. Techniques used to limit nephrotoxicity during CisPt treatment are not fully effective; about a third of patients experience AKI. New nephroprotective strategies, including pharmacological approaches, must be developed. Objective: The present study investigated the nephroprotective potential of Angelica sinensis (Oliv.) Diels (Apiaceae) root towards CisPt tubulotoxicity. Materials and methods: HK-2 cells were incubated with CisPt (10 mM) and/or with a methanolic extract of A. sinensis (AS). Nephroprotective capacity was evaluated by means of cellular viability (resazurin assay) and apoptosis (annexin-V/PI staining), oxidative stress generation (H 2 DCF-DA oxidation), Ki-67 index (immunofluorescence), cell cycle analysis (DNA staining), cell migration rate (scratch assay), extracellular matrix deposition (collagen determination), and b-catenin relocalization. Results: CisPt decreased cell viability [76% versus Ctrl], which was associated with an increased apoptosis. Simultaneous treatment with 50 mg/ml AS enhanced cell survival [84% versus Ctrl] and decreased the apoptosis rate. AS could not alleviate CisPt-induced oxidative stress; but doses of 5 and 50 mg/ml raised the Ki-67 index [135 and 244% versus Ctrl] and cell migration rates [1.2 and 1.3-fold versus Ctrl]. Finally, both doses of AS limited the amount of collagen deposition [121.6 and 119.6% for 5 and 50 mg/ml, respectively, versus 131.0% for CisPt-treated cells] and prevented the relocalization of b-catenin from the membrane to the nucleus. Conclusion: These results confirm the nephroprotective potential of A. sinensis and require further investigations aiming at identifying its active compounds.

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Aqueous-ethanolic extract of morel mushroom mycelium Morchella esculenta, protects cisplatin and gentamicin induced nephrotoxicity in mice

Cited by 85 publications

References 50 publications

Protective Effect of Quercetin against Gentamicin-Induced Nephrotoxicity in Rats

Protective Effect of Quercetin against Gentamicin-Induced Nephrotoxicity in Rats

Nephroprotective activity ofMacrothelypteris oligophlebiarhizomes ethanol extract

Potential nephroprotective effects of the Chinese herbAngelica sinensisagainst cisplatin tubulotoxicity

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