Aquatic toxicology of fluoxetine: Understanding the knowns and the unknowns

Stewart, Adam; Grossman, Leah; Nguyen, Michael; Maximino, Caio; Rosemberg, Denis B.; Echevarria, David J.; Kalueff, Allan V.

doi:10.1016/j.aquatox.2014.08.014

Cited by 48 publications

(24 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Variability in responsiveness amongst studies has been highlighted, and it is, therefore, important to be mindful when interpreting results and forming conclusions using behavioural analysis and other novel approaches to toxicity testing . However, the application of innovative approaches for quantifying behavioural endpoints has also shed enormous light on the possibility for common environmental pollutants to elicit adverse sub-lethal effects in fish (Stewart et al, 2014). The present study is the first, to our knowledge, to explore activity levels in fish exposed to PPCPs alone or in the mixture using the MFB.…”

Section: Discussionmentioning

confidence: 99%

Diurnal activity patterns as a sensitive behavioural outcome in fish: effect of short‐term exposure to treated sewage and a sub‐lethal PPCP mixture

Melvin

Buck

Fabbro

2016

J of Applied Toxicology

View full text Add to dashboard Cite

Sub-lethal toxicological responses are common occurrences in aquatic animals exposed to sewage wastewater and organic wastewater contaminants. Behavioural alterations are particularly sensitive indicators of sub-lethal toxicological stress in animals exposed to various pollutants, and often correlate with higher-level outcomes. Diurnal activity patterns in many fish species are sensitive to changes in natural biotic factors, but few studies have explored how environmental pollutants influence such rhythms. We investigated diurnal activity patterns in the mosquitofish (Gambusia holbrooki), after exposure to UV-treated sewage and a mixture of key contaminants identified through chemical analysis and subsequent risk-based prioritization of the wastewater. Exposure to 50% and 100% wastewater abolished daytime activity levels in male, but not female fish. Chemical analysis identified fluoxetine, diazinon and triclosan above their reported predicted-no-effect-concentrations (PNECs), and fish were thus exposed to a mixture of these compounds at 1, 10 and 100 μg l -1. Behavioural responses were highly consistent between fish exposed to wastewater and the contaminant mixture, indicating that these prioritized contaminants are indeed likely contributing to the observed effects. Effective concentrations of the mixture were considerably lower than those reported as eliciting behavioural effects in previous studies exploring each of these compounds alone. Results warn of the potential for negative higher-level consequences associated with exposures of fish to common organic wastewater contaminants, as altered diurnal activity patterns could conceivably scale-up to influence performance including foraging success and predator avoidance. Further research is necessary to increase our understanding of linkages between alterations to diurnal activities and effects at the population level.

show abstract

Section: Discussionmentioning

confidence: 99%

Diurnal activity patterns as a sensitive behavioural outcome in fish: effect of short‐term exposure to treated sewage and a sub‐lethal PPCP mixture

Melvin

Buck

Fabbro

2016

J of Applied Toxicology

View full text Add to dashboard Cite

show abstract

“…The development of mutant zebrafish provides a more stable behavioural phenotype, because rather than produce a knockdown of a given gene, it completely eliminates the target gene product (Amsterdam and Hopkins, ; Stewart et al ., ). Mutants are created through retroviral insertional mutagenesis, wherein DNA basepairs are integrated into the organism's pre‐existing DNA (Amsterdam and Hopkins, ) or through chemical mutagenesis.…”

Section: Perspectives On Small Molecule and Genetic Screening In Zebrmentioning

confidence: 97%

“…Neurochemistry is generally conserved across vertebrate species, as they share major neurotransmitters, receptors and transporters (Panula et al, , ; Alsop and Vijayan, ). Thus, zebrafish are sensitive to major classes of pharmacological agents, such as psychostimulants (Ninkovic and Bally‐Cuif, ), opiates (Lau et al, ), ethanol (Tran et al, ), hallucinogens (Stewart et al, ), anxiolytics (Bencan et al, ), antidepressants (Stewart et al, ) and antiopsychotics (Bruni et al, ). The spatial and temporal distribution of major neurotransmitter systems in zebrafish is also similar to that of mammals and has been well described in zebrafish for glutamate, GABA, acetylcholine, dopamine, 5‐HT, noradrenaline and histamine (Stewart et al, ).…”

Section: Zebrafish Cnsmentioning

confidence: 99%

Zebrafish models in neuropsychopharmacology and CNS drug discovery

Khan

Collier

Meshalkina

et al. 2017

British J Pharmacology

146

View full text Add to dashboard Cite

Despite the high prevalence of neuropsychiatric disorders, their aetiology and molecular mechanisms remain poorly understood. The zebrafish (Danio rerio) is increasingly utilized as a powerful animal model in neuropharmacology research and in vivo drug screening. Collectively, this makes zebrafish a useful tool for drug discovery and the identification of disordered molecular pathways. Here, we discuss zebrafish models of selected human neuropsychiatric disorders and drug-induced phenotypes. As well as covering a broad range of brain disorders (from anxiety and psychoses to neurodegeneration), we also summarize recent developments in zebrafish genetics and small molecule screening, which markedly enhance the disease modelling and the discovery of novel drug targets.

show abstract

“…In terms of ecotoxicity, carbamazepine, diclofenac, and metoprolol have been evidenced to cause growth retardation and heart abnormalities in fish embryos (28). Fluoxetine seems to cause endocrine-mediated reproduction dysfunction and developmental abnormalities, also in fish embryos (29,30). Antibiotic residues in aquatic systems have clearly showed a potential to induce resistance in bacterial strains and become a serious threat for public health (31,32).…”

Section: Methodsmentioning

confidence: 99%

Levels of pharmaceuticals in Slovene municipal and hospital wastewaters: a preliminary study

Klančar

Trontelj

Kristl

et al. 2016

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

Pharmaceuticals in wastewater have clearly raised concern and a broad range of analytical methods has been used to assess the risk as accurately as possible. The aim of our study was to measure and compare the concentrations of atorvastatin, bisoprolol, carbamazepine, ciprofloxacin, clofibric acid, diclofenac, fluoxetine, metoprolol, and sertraline in wastewater samples taken from one municipal and one hospital wastewater treatment plant in Slovenia and to predict the potential environmental burden using the risk quotient. In both effluents only clofibric acid and fluoxetine were not detected. The measured concentrations of the remaining seven pharmaceuticals varied between the ng L -1 and the µg L -1 range. Hospital effluent showed higher concentrations, except for diclofenac and carbamazepine. However, high risk quotient was found only for ciprofloxacin and diclofenac in both municipal and hospital effluent. In conclusion, our method can provide a useful tool for systematic monitoring of pharmaceuticals commonly found in wastewater, which will enable a reliable assessment of the risks for the aquatic biota and humans. Knowing the risks will help to plan wastewater treatment and preserve our environment.

show abstract

Aquatic toxicology of fluoxetine: Understanding the knowns and the unknowns

Cited by 48 publications

References 81 publications

Diurnal activity patterns as a sensitive behavioural outcome in fish: effect of short‐term exposure to treated sewage and a sub‐lethal PPCP mixture

Diurnal activity patterns as a sensitive behavioural outcome in fish: effect of short‐term exposure to treated sewage and a sub‐lethal PPCP mixture

Zebrafish models in neuropsychopharmacology and CNS drug discovery

Levels of pharmaceuticals in Slovene municipal and hospital wastewaters: a preliminary study

Contact Info

Product

Resources

About