Aquaporin Pathways and Mucin Secretion of Bowman's Glands Might Protect the Olfactory Mucosa

Solbu, Tom Tallak; Holen, Torgeir

doi:10.1093/chemse/bjr063

Cited by 45 publications

(43 citation statements)

References 35 publications

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“…Aquaporin 5, a water channel, is expressed in Bowman’s gland and supporting cells’ microvilli [46,64]. We observed aquaporin 5 expression in the duct cells and secretory acinar cells of Bowman’s gland both in TMEM16A +/+ and TMEM16A -/- littermate mice (Fig 8).…”

Section: Discussionmentioning

confidence: 76%

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Development of the Olfactory Epithelium and Nasal Glands in TMEM16A-/- and TMEM16A+/+ Mice

et al. 2015

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TMEM16A/ANO1 is a calcium-activated chloride channel expressed in several types of epithelia and involved in various physiological processes, including proliferation and development. During mouse embryonic development, the expression of TMEM16A in the olfactory epithelium is dynamic. TMEM16A is expressed at the apical surface of the entire olfactory epithelium at embryonic day E12.5 while from E16.5 its expression is restricted to a region near the transition zone with the respiratory epithelium. To investigate whether TMEM16A plays a role in the development of the mouse olfactory epithelium, we obtained the first immunohistochemistry study comparing the morphological properties of the olfactory epithelium and nasal glands in TMEM16A-/- and TMEM16A+/+ littermate mice. A comparison between the expression of the olfactory marker protein and adenylyl cyclase III shows that genetic ablation of TMEM16A did not seem to affect the maturation of olfactory sensory neurons and their ciliary layer. As TMEM16A is expressed at the apical part of supporting cells and in their microvilli, we used ezrin and cytokeratin 8 as markers of microvilli and cell body of supporting cells, respectively, and found that morphology and development of supporting cells were similar in TMEM16A-/- and TMEM16A+/+ littermate mice. The average number of supporting cells, olfactory sensory neurons, horizontal and globose basal cells were not significantly different in the two types of mice. Moreover, we also observed that the morphology of Bowman’s glands, nasal septal glands and lateral nasal glands did not change in the absence of TMEM16A. Our results indicate that the development of mouse olfactory epithelium and nasal glands does not seem to be affected by the genetic ablation of TMEM16A.

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Section: Discussionmentioning

confidence: 76%

“…We investigated the expression of TMEM16A in Bowman’s glands, nasal septal glands, and lateral nasal glands, whose localization is schematically represented in Fig 1A, and used aquaporin 5 as a marker for these glands [46]. …”

Section: Resultsmentioning

confidence: 99%

Development of the Olfactory Epithelium and Nasal Glands in TMEM16A-/- and TMEM16A+/+ Mice

et al. 2015

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“…The luminal membranes of glandular duct cells and supporting cells both contain ANO 1 chloride channels together with the aquaporin water channel AQP 5 ( Fig. 4g; see also Solbu and Holen 2011). This co-localization points to a central role of ANO 1 channels in water homeostasis for the mucus that covers the MOE surface.…”

Section: Main Olfactory Epitheliummentioning

confidence: 82%

“…The epithelial Na + channel ENaC, whose α subunit is expressed in most nasal surface and gland epithelia (Menco et al 1998;Rochelle et al 2000), provides the corresponding cation pathway. Apical water transport seems to be mainly mediated by the aquaporin isoform 5 (AQP 5), whereas the AQP 3 and AQP 4 isoforms secure water uptake across baso-lateral membranes (Ablimit et al 2006;Lu et al 2008;Solbu and Holen 2011). In its physiological role, ANO 1 seems to complement the action of the cystic fibrosis transmembrane conductance regulator (CFTR) protein, which is a cAMP-activated Cl − channel.…”

Section: Discussionmentioning

confidence: 99%

Expression patterns of anoctamin 1 and anoctamin 2 chloride channels in the mammalian nose

et al. 2012

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Calcium-activated chloride channels are expressed in chemosensory neurons of the nose and contribute to secretory processes and sensory signal transduction. These channels are thought to be members of the family of anoctamins (alternative name: TMEM16 proteins), which are opened by micromolar concentrations of intracellular Ca(2+). Two family members,ANO 1 (TMEM16A) and ANO 2 (TMEM16B), are expressed in the various sensory and respiratory tissues of the nose.We have examined the tissue specificity and sub-cellular localization of these channels in the nasal respiratory epithelium and in the five chemosensory organs of the nose: the main olfactory epithelium, the septal organ of Masera, the vomeronasal organ, the Grueneberg ganglion and the trigeminal system. We have found that the two channels show mutually exclusive expression patterns. ANO 1 is present in the apical membranes of various secretory epithelia in which it is co-localized with the water channel aquaporin 5. It has also been detected in acinar cells and duct cells of subepithelial glands and in the supporting cells of sensory epithelia. In contrast, ANO 2 expression is restricted to chemosensory neurons in which it has been detected in microvillar and ciliary surface structures. The different expression patterns of ANO 1 and ANO 2 have been observed in the olfactory, vomeronasal and respiratory epithelia. No expression has been detected in the Grueneberg ganglion or trigeminal sensory fibers. On the basis of this differential expression, we derive the main functional features of ANO 1 and ANO 2 chloride channels in the nose and suggest their significance for nasal physiology.

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“…Scattered throughout the OE are the non-neuronal microvillar cells and Bowman’s glands. Bowman’s glands consist of clustered acinar cells located under the OE in the lamina propria, linked to ducts that span the epithelium to transport mucus to the apical surface3. At least three types of microvillar cells have been described in the OE4.…”

mentioning

confidence: 99%

Ascl3 transcription factor marks a distinct progenitor lineage for non-neuronal support cells in the olfactory epithelium

Weng

Vinjamuri

Ovitt

2016

Sci Rep

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The olfactory epithelium (OE) is composed of olfactory sensory neurons (OSNs), sustentacular supporting cells, and several types of non-neuronal cells. Stem and progenitor cells are located basally, and are the source of all cell types needed to maintain OE homeostasis. Here, we report that Ascl3, a basic helix-loop-helix transcription factor, is expressed in the developing OE. Lineage tracing experiments demonstrate that the non-neuronal microvillar cells and Bowman’s glands are exclusively derived from Ascl3+ progenitor cells in the OE during development. Following chemically-induced injury, Ascl3 expression is activated in a subset of horizontal basal cells (HBCs), which repopulate all microvillar cells and Bowman’s glands during OE regeneration. After ablation of Ascl3-expressing cells, the OE can regenerate, but lacks the non-neuronal microvillar and Bowman’s gland support cells. These results demonstrate that Ascl3 marks progenitors that are lineage-committed strictly to microvillar cells and Bowman’s glands, and highlight the requirement for these cell types to support OE homeostasis.

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Aquaporin Pathways and Mucin Secretion of Bowman's Glands Might Protect the Olfactory Mucosa

Cited by 45 publications

References 35 publications

Development of the Olfactory Epithelium and Nasal Glands in TMEM16A-/- and TMEM16A+/+ Mice

Development of the Olfactory Epithelium and Nasal Glands in TMEM16A-/- and TMEM16A+/+ Mice

Expression patterns of anoctamin 1 and anoctamin 2 chloride channels in the mammalian nose

Ascl3 transcription factor marks a distinct progenitor lineage for non-neuronal support cells in the olfactory epithelium

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