Apyrase and α-glucosidase in the salivary glands of Aedes albopictus

Marinotti, Osvaldo; Brito, Marylene de; Moreira, Cristina K.

doi:10.1016/0305-0491(95)02035-7

Cited by 64 publications

(50 citation statements)

References 14 publications

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“…These values are consistent with the morphological differences observed between the salivary glands of males and females. These morphological and protein content differences have been observed in other mosquito species and were related with the different feeding habits of males and females 18,21,24,25 . Females feed on both sugar and blood, whereas males feed on sugar only 43 .…”

Section: Discussionsupporting

confidence: 60%

“…The molecular masses of these major protein bands were estimated at 63, 44, 43, 35, 33, 30 and 18 kilodaltons (kDa), consecutively. The male gland protein profile differed from the female one and the protein content was lower Apyrase activity was determined by inorganic phosphate production from ADP hydrolysis as described by MARINOTTI et al 21 (n = 20) a ; alpha-glucosidase activity was determined by the release of glucose from sucrose by the glucose oxidase-peroxidase method 7 (n = 20) b . The total activity in the glands was set as 100%.…”

Section: Resultsmentioning

confidence: 99%

“…The distal region of the lateral lobes is necessary for mosquito blood feeding. The apyrase, synthesized and accumulated in the female-specific region of the gland, has been related to inhibition of the platelet aggregation, which facilitates blood ingestion by the mosquitoes 21,38 . This enzyme is secreted by the salivary glands only during blood feeding.…”

Section: Discussionmentioning

confidence: 99%

“…aegypti and Ae. albopictus indicates that the proximal regions of the lateral lobes of the mosquitoes' salivary glands synthesize and accumulate molecules that help in sugar solubilization, ingestion and digestion 5,21 . The Mal-I gene expressed exclusively in the cells of the proximal region of the Ae.…”

Section: Discussionmentioning

confidence: 99%

“…Apyrase enzymatic activity assay: Apyrase activity was determined as previously described by MARINOTTI et al 21 . One µL of salivary gland homogenate was incubated at 37 °C with 99 µL of 50 mM Tris-HCl buffer (pH 9.0), containing 100 mM NaCl, 5 mM CaCl 2 , 2 mM Adenosine S'-Diphosphate (ADP) and 20 mM β-mercaptoethanol in a flat-bottom microtiter plate.…”

Section: Protein Quantificationmentioning

confidence: 99%

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Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Jariyapan

Choochote

Jitpakdi

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSalivary gland proteins of the human malaria vector, Anopheles dirus B were determined and analyzed. The amount of salivary gland proteins in mosquitoes aged between 3 -10 days was approximately 1.08 ± 0.04 µg/female and 0.1 ± 0.05 µg/male. The salivary glands of both sexes displayed the same morphological organization as that of other anopheline mosquitoes. In females, apyrase accumulated in the distal regions, whereas alpha-glucosidase was found in the proximal region of the lateral lobes. This differential distribution of the analyzed enzymes reflects specialization of different regions for sugar and blood feeding. SDS-PAGE analysis revealed that at least seven major proteins were found in the female salivary glands, of which each morphological region contained different major proteins. Similar electrophoretic protein profiles were detected comparing unfed and blood-fed mosquitoes, suggesting that there is no specific protein induced by blood. Two-dimensional polyacrylamide gel analysis showed the most abundant salivary gland protein, with a molecular mass of approximately 35 kilodaltons and an isoelectric point of approximately 4.0. These results provide basic information that would lead to further study on the role of salivary proteins of An. dirus B in disease transmission and hematophagy.

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Section: Discussionsupporting

confidence: 60%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Protein Quantificationmentioning

confidence: 99%

See 3 more Smart Citations

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Jariyapan

Choochote

Jitpakdi

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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Salivary gland proteins of the mosquitoCulex quinquefasciatus

Nascimento¹,

Malafronte²,

Marinotti³

2000

Arch. Insect Biochem. Physiol.

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Several properties of the salivary glands of Culex quinquefasciatus mosquitoes were analysed. The amount of protein in female salivary glands increased from 0.26 μg on day one after emergence to about 1.4 μg on day seven. The major polypeptides found in the female salivary glands had molecular weights of 35.7, 28.3, and 20.5 kDa. Antibodies produced by mice immunized by bites of Culex quinquefasciatus female mosquitoes reacted with the 35.7 and 28.3 kDa polypeptides, showing that these molecules were secreted by mosquitoes during blood feeding. The salivary glands of C. Quinquefasciatus females displayed the same morphological and biochemical organization as that of Aedes aegypti mosquitoes, accumulating apyrase in the distal portions and α‐glucosidase in the proximal portions of the gland. Arch. Insect Biochem. Physiol. 43:9–15, 2000. © 2000 Wiley‐Liss, Inc.

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Salivary gland proteins of the mosquito Culex quinquefasciatus

Nascimento¹,

Malafronte²,

Marinotti³

2000

Arch. Insect Biochem. Physiol.

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Several properties of the salivary glands of Culex quinquefasciatus mosquitoes were analysed. The amount of protein in female salivary glands increased from 0.26 microg on day one after emergence to about 1.4 microg on day seven. The major polypeptides found in the female salivary glands had molecular weights of 35.7, 28.3, and 20.5 kDa. Antibodies produced by mice immunized by bites of Culex quinquefasciatus female mosquitoes reacted with the 35.7 and 28.3 kDa polypeptides, showing that these molecules were secreted by mosquitoes during blood feeding. The salivary glands of C. Quinquefasciatus females displayed the same morphological and biochemical organization as that of Aedes aegypti mosquitoes, accumulating apyrase in the distal portions and alpha-glucosidase in the proximal portions of the gland. Arch.

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Apyrase and α-glucosidase in the salivary glands of Aedes albopictus

Cited by 64 publications

References 14 publications

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Salivary gland proteins of the mosquitoCulex quinquefasciatus

Salivary gland proteins of the mosquito Culex quinquefasciatus

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