Salivary gland proteins of the mosquito Culex quinquefasciatus

Nascimento, Eva Pereira; Malafronte, Rosely dos Santos; Marinotti, Osvaldo

doi:10.1002/(sici)1520-6327(200001)43:1<9::aid-arch2>3.3.co;2-u

Cited by 10 publications

(12 citation statements)

References 15 publications

(26 reference statements)

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“…These values are consistent with the morphological differences observed between the salivary glands of males and females. These morphological and protein content differences have been observed in other mosquito species and were related with the different feeding habits of males and females 18,21,24,25 . Females feed on both sugar and blood, whereas males feed on sugar only 43 .…”

Section: Discussionsupporting

confidence: 57%

“…darlingi 23 and Cx. quinquefasciatus 25 , demonstrated accumulation preferentially in the distal region and proximal region of the lateral lobes, respectively. The salivary apyrase and alpha-glucosidase of An.…”

Section: Discussionmentioning

confidence: 98%

“…darlingi 23,24 , Culex pipiens 3 , Cx. quinquefasciatus 25 , Ae. togoi 18 and Armigeres subalbatus 40 .…”

Section: Introductionunclassified

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Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Jariyapan

Choochote

Jitpakdi

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSalivary gland proteins of the human malaria vector, Anopheles dirus B were determined and analyzed. The amount of salivary gland proteins in mosquitoes aged between 3 -10 days was approximately 1.08 ± 0.04 µg/female and 0.1 ± 0.05 µg/male. The salivary glands of both sexes displayed the same morphological organization as that of other anopheline mosquitoes. In females, apyrase accumulated in the distal regions, whereas alpha-glucosidase was found in the proximal region of the lateral lobes. This differential distribution of the analyzed enzymes reflects specialization of different regions for sugar and blood feeding. SDS-PAGE analysis revealed that at least seven major proteins were found in the female salivary glands, of which each morphological region contained different major proteins. Similar electrophoretic protein profiles were detected comparing unfed and blood-fed mosquitoes, suggesting that there is no specific protein induced by blood. Two-dimensional polyacrylamide gel analysis showed the most abundant salivary gland protein, with a molecular mass of approximately 35 kilodaltons and an isoelectric point of approximately 4.0. These results provide basic information that would lead to further study on the role of salivary proteins of An. dirus B in disease transmission and hematophagy.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 98%

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Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Jariyapan

Choochote

Jitpakdi

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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“…A similar difference in lobe size of salivary glands has been observed mosquito females, which exhibit three lobes of different sizes (43,44). Moreover, the results show a decrease in gland size between these two species of Lutzomyia post blood feeding in comparison with sugar solution feeding.…”

Section: Resultssupporting

confidence: 78%

Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

et al. 2010

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Introduction. Leishmaniasis is a vector-borne disease transmitted by the intradermal inoculation of Leishmania (Kinetoplastida: Trypanosomatidae) promastigotes together with saliva during the bite of an infected sand fly. Objective. The salivary glands were compared from two vector species, Lutzomyia ovallesi (Ortiz,1952) and Lutzomyia migonei (França,1920) (Diptera: Psychodidae). Material and methods. Protein profiles by SDS PAGE of salivary glands were compared among species as well as their development at several times post feeding. First, mice were immunized to salivary proteins by exposure to biting by L. ovallesi and of L. migonei. Antibodies in these mice against salivary gland-specific proteins were evaluated by immunoblotting. Results. No apparent change was revealed in the kinetic expression of salivary proteins induced by the different physiological states post feeding. Qualitative and quantitative variations were detected in16-18 polypeptides with molecular weights ranging from 6 to 180 kDa. Species-specific proteins were demonstrated for L. migonei and L. ovallesi. In addition, antibodies against salivary gland specific proteins were found in mice immunized by the saliva of both species. Conclusion. Basic information was obtained concerning the nature of salivary gland proteins of L. migonei and L. ovallesi. This information helps to elucidate the role of salivary proteins and their potential as effective tools in screening risk factors in human and other vertebrate hosts.

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“…Aedes aegypti (Champagne et al 1995b), Anopheles (Arca et al 1999) and Culex mosquitoes ( Fig. 1e) (Nascimento et al 2000) present in their saliva apyrases from the same family of 5'-nucleotidases. A novel apyrase enzyme sequence was found recently in the salivary glands of the haematophagous bed bug Cimex lectularius (Valenzuela et al 1998) and homologous sequences were found in the sand flies Lutzomia longipalpis (Charlab et al 1999) and Phlebotomus papatasi , indicating that this family of enzymes is widespread among arthropod species (Fig.…”

Section: Platelet Aggregationmentioning

confidence: 99%

Haematophagous arthropod saliva and host defense system: a tale of tear and blood

Andrade

Teixeira

Barral

et al. 2005

An. Acad. Bras. Ciênc.

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The saliva from blood-feeding arthropod vectors is enriched with molecules that display diverse functions that mediate a successful blood meal. They function not only as weapons against host's haemostatic, inflammatory and immune responses but also as important tools to pathogen establishment. Parasites, virus and bacteria taking advantage of vectors' armament have adapted to facilitate their entry in the host. Today, many salivary molecules have been identifi ed and characterized as new targets to the development of future vaccines. Here we focus on current information on vector's saliva and the molecules responsible to modify host's hemostasis and immune response, also regarding their role in disease transmission.

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Salivary gland proteins of the mosquito Culex quinquefasciatus

Cited by 10 publications

References 15 publications

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Salivary gland proteins of the human malaria vector, Anopheles dirus B (Diptera: Culicidae)

Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

Haematophagous arthropod saliva and host defense system: a tale of tear and blood

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