APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria

Dirkzwager, Roderick M.; Kinghorn, Andrew B.; Richards, Jack S.; Tanner, Julian A.

doi:10.1039/c5cc00438a

Cited by 53 publications

(51 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Table 1 summarizes and compares recent studies on the diagnosis of PfLDH. Recently, many studies have developed high-sensitivity, high-selectivity, and cost-effective diagnostics based on aptamers or nanoparticles [31][32][33][34][35]. Nanoparticles, such as novel-metal nanoparticles and magnetic nanoparticles, are applied for lateral flow immunoassay or combined with aptamers to improved diagnostic performance.…”

Section: Performance Of the Microfluidic Microplate For Pfldh Immunoamentioning

confidence: 99%

Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase

et al. 2020

View full text Add to dashboard Cite

This work reports on a rapid diagnostic platform for the detection of Plasmodium falciparum lactate dehydrogenase (PfLDH), a representative malaria biomarker, using a microfluidic microplate-based immunoassay. In this study, the microfluidic microplate made it possible to diagnose PfLDH with a small volume of sample (only 5 μL) and short time (< 90 min) compared to conventional immunoassays such as enzyme-linked immunosorbent assay (ELISA). Moreover, the diagnostic performance of PfLDH showed high sensitivity, specificity, and selectivity (i.e., 0.025 pg/μL in phosphate-buffered saline and 1 pg/μL in human serum). The microfluidic-based microplate sensing platform has the potential to adapt simple, rapid, and accurate diagnoses to the practical detection of malaria.

show abstract

Section: Performance Of the Microfluidic Microplate For Pfldh Immunoamentioning

confidence: 99%

Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Label‐free detection is another aspect of aptamer‐based microfluidic detection (Figure c). A Plasmodium falciparum lactate dehydrogenase (PfLDH) specific aptamer was identified by our team (Tanner's research group) and an aptamer‐tethered enzyme capture (APTEC) assay which bases detection on the intrinsic enzyme activity of the captured PfLDH malaria biomarker protein was developed . It has since been incorporated to a range different detection formats 9d,e,82.…”

Section: Microfluidic Selection Of Aptamersmentioning

confidence: 99%

Microfluidic Technology for Nucleic Acid Aptamer Evolution and Application

et al. 2019

Self Cite

View full text Add to dashboard Cite

The intersection of microfluidics and aptamer technologies holds particular promise for rapid progress in a plethora of applications across biomedical science and other areas. Here, the influence of microfluidics on the field of aptamers, from traditional capillary electrophoresis approaches through innovative modern‐day approaches using micromagnetic beads and emulsion droplets, is reviewed. Miniaturizing aptamer‐based bioassays through microfluidics has the potential to transform diagnostics and embedded biosensing in the coming years.

show abstract

“…Several biomarkers for diagnosis of malaria have been investigated [13] among which Plasmodium-specific lactate dehydrogenase has emerged as a promising biomarker for malaria detection owing to its subtle structural and kinetic differences from the mammalian counterpart, leading to an increased interest in development of sensors that target this enzyme [14][15][16][17][18]. Here, we report a novel ssDNA aptamer developed against Plasmodium falciparum lactate dehydrogenase and its application potential to detect this biomarker through an aromatic cationic surfactant-based assay using gold nanoparticles as the optical sensing platform.…”

Section: Introductionmentioning

confidence: 99%

Aromatic Surfactant as Aggregating Agent for Aptamer-Gold Nanoparticle-Based Detection of Plasmodium Lactate Dehydrogenase

et al. 2016

View full text Add to dashboard Cite

A novel ssDNA aptamer (P38), with a 40 mer random region flanked by primer-binding sites on both sides, targeting Plasmodium falciparum lactate dehydrogenase (PfLDH) has been developed through systematic evolution of ligands by exponential enrichment (SELEX), including counter SELEX against human lactate dehydrogenase A and B (hLDH A and B). The 2D structure of P38 shows the presence of three stem loops with a δG of -9.18 kcal/mol. EMSA studies on P38 complexes with the increasing concentration of PfLDH revealed a dissociation constant of 0.35 µM. P38 has been exploited for the quantitative detection of PfLDH using cationic surfactant-mediated aggregation of gold nanoparticles (16-nm diameter) as an optical probe. Among the three different cationic surfactants, characterized by different hydrocarbon tail groups, benzalkonium chloride (BCK) was found to be most efficient with a limit of detection of 281 ± 11 pM. This BCK-based approach with the novel highly selective aptamer provides simple and sensitive detection of PfLDH in the clinically relevant range.

show abstract

APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria

Cited by 53 publications

References 38 publications

Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase

Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase

Microfluidic Technology for Nucleic Acid Aptamer Evolution and Application

Aromatic Surfactant as Aggregating Agent for Aptamer-Gold Nanoparticle-Based Detection of Plasmodium Lactate Dehydrogenase

Contact Info

Product

Resources

About