Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase

Lee, Wang Sik; Kang, Taejoon; Kwak, Kyung Jin; Park, Kyoungsook; Yi, So Yeon; Lee, Ui Jin; Shin, Yong Beom; Jeong, Jinyoung

doi:10.1186/s40580-020-00223-w

Cited by 15 publications

(8 citation statements)

References 33 publications

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“…Furthermore, the functionalization procedure of PIT can be carried out by a simple UV lamp in a few minutes, thus we anticipate that such an approach can be carried out in low resource settings which would be critical for many malaria diagnostic applications. Therefore, we foresee that our approach may provide a solution to the cold transport challenges common to the presently used immunochromatographic lateral flow assays 45 – 47 .…”

Section: Discussionmentioning

confidence: 99%

“…The conventional serologic antibody-based rapid diagnostic tests rely upon the Pf LDH detection in pretreated blood and offer rapid and cost-effective malaria diagnosis. However, the poor LOD as well as the transportation and storage difficulties of pre-functionalized devices in tropical environment make such tests unfit for early diagnosis and screening 45 – 47 . The ultrasensitive PEF-based device in combination with a unique functionalization procedure (PIT), which can be accomplished in a few minutes, results in an immunosensor suitable for detecting Pf LDH at femtomolar level in the whole blood without any pretreatment and preconcentration steps.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

et al. 2020

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Development of plasmonic biosensors combining reliability and ease of use is still a challenge. Gold nanoparticle arrays made by block copolymer micelle nanolithography (BCMN) stand out for their scalability, cost-effectiveness and tunable plasmonic properties, making them ideal substrates for fluorescence enhancement. Here, we describe a plasmon-enhanced fluorescence immunosensor for the specific and ultrasensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH)—a malaria marker—in whole blood. Analyte recognition is realized by oriented antibodies immobilized in a close-packed configuration via the photochemical immobilization technique (PIT), with a top bioreceptor of nucleic acid aptamers recognizing a different surface of PfLDH in a sandwich conformation. The combination of BCMN and PIT enabled maximum control over the nanoparticle size and lattice constant as well as the distance of the fluorophore from the sensing surface. The device achieved a limit of detection smaller than 1 pg/mL (<30 fM) with very high specificity without any sample pretreatment. This limit of detection is several orders of magnitude lower than that found in malaria rapid diagnostic tests or even commercial ELISA kits. Thanks to its overall dimensions, ease of use and high-throughput analysis, the device can be used as a substrate in automated multi-well plate readers and improve the efficiency of conventional fluorescence immunoassays.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

et al. 2020

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“…The microfluidic microplate is a spiral microfluidic channel with 1.5 times larger surface area and 50 times larger surface area-to-volume ratio compared to conventional ELISA plates. The detection limit is as low as 6.25 × 10 −3 U/L [ 43 ]. However, the UV spectrum also will be easily affected by the external environment, such as the color of the sample [ 70 ].…”

Section: Ldh Detection Methodsmentioning

confidence: 99%

“…For instance, numerous studies have reported LDH as a prognostic biomarker for colorectal cancer [ 32 , 33 ], lung cancer [ 34 , 35 ], melanoma [ 36 , 37 ], renal cell cancer [ 38 , 39 ] and hepatocellular carcinoma [ 40 , 41 ]. Many studies have also shown that LDH is also a biomarker for malaria [ 42 , 43 , 44 ]. Moreover, LDH activity is an important clinical guide to the choice of chemotherapy and helps to identify whether a pleural effusion is benign or malignant [ 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

Current Status and Future Perspectives of Lactate Dehydrogenase Detection and Medical Implications: A Review

Zhou

Yang

2022

Biosensors

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The demand for glucose uptake and the accompanying enhanced glycolytic energy metabolism is one of the most important features of cancer cells. Unlike the aerobic metabolic pathway in normal cells, the large amount of pyruvate produced by the dramatic increase of glycolysis in cancer cells needs to be converted to lactate in the cytoplasm, which cannot be done without a large amount of lactate dehydrogenase (LDH). This explains why elevated serum LDH concentrations are usually seen in cancer patient populations. LDH not only correlates with clinical prognostic survival indicators, but also guides subsequent drug therapy. Besides their role in cancers, LDH is also a biomarker for malaria and other diseases. Therefore, it is urgent to develop methods for sensitive and convenient LDH detection. Here, this review systematically summarizes the clinical impact of lactate dehydrogenase detection and principles for LDH detection. The advantages as well as limitations of different detection methods and the future trends for LDH detection were also discussed.

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“…Cefadroxil and amoxicillin, which have very similar structures, remarkably showed adequate separation. This sensor has also been used to diagnose diseases [95][96][97][98][99].…”

Section: Genetic Engineering For Functional Peptide Generation On M13...mentioning

confidence: 99%

The development progress of multi-array colourimetric sensors based on the M13 bacteriophage

et al. 2023

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Techniques for detecting chemicals dispersed at low concentrations in air continue to evolve. These techniques can be applied not only to manage the quality of agricultural products using a post-ripening process but also to establish a safety prevention system by detecting harmful gases and diagnosing diseases. Recently, techniques for rapid response to various chemicals and detection in complex and noisy environments have been developed using M13 bacteriophage-based sensors. In this review, M13 bacteriophage-based multi-array colourimetric sensors for the development of an electronic nose is discussed. The self-templating process was adapted to fabricate a colour band structure consisting of an M13 bacteriophage. To detect diverse target chemicals, the colour band was utilised with wild and genetically engineered M13 bacteriophages to enhance their sensing abilities. Multi-array colourimetric sensors were optimised for application in complex and noisy environments based on simulation and deep learning analysis. The development of a multi-array colourimetric sensor platform based on the M13 bacteriophage is likely to result in significant advances in the detection of various harmful gases and the diagnosis of various diseases based on exhaled gas in the future.

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Simple, rapid, and accurate malaria diagnostic platform using microfluidic-based immunoassay of Plasmodium falciparum lactate dehydrogenase

Cited by 15 publications

References 33 publications

Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

Current Status and Future Perspectives of Lactate Dehydrogenase Detection and Medical Implications: A Review

The development progress of multi-array colourimetric sensors based on the M13 bacteriophage

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