Aptasensor for the detection of Methicillin resistant Staphylococcus aureus on contaminated surfaces

Despite progress in fighting infectious diseases, human pathogenesis and death caused by infectious diseases remain relatively high worldwide exceeding that of cancer and cardiovascular diseases. Human adenovirus (HAdV) infects cells of the upper respiratory tract causing flu-like symptoms that are accompanied by pain and inflammation. Diagnosis of HAdV is commonly achieved by conventional methods such as viral cultures, immunoassays, and polymerase chain reaction (PCR) techniques. However, there are a variety of problems with conventional methods including slow isolation and propagation, inhibition by neutralizing antibodies, low sensitivity of immunoassays, and the diversity of HAdV strains for the PCR technique. Herein, we report the development and evaluation of a novel, simple, and reliable nanobased immunosensing technique for the rapid detection of human adenoviruses (HAdVs) that cause eye infections. This rapid and low-cost assay can be used for screening and quantitative tests with a detection limit of 10 2 pfu/mL in less than 2 min. The sensing platform is based on a sandwich assay that can detect HAdVs visually by a color change. Sensor specificity was demonstrated using other common viral antigens, including Flu A, Flu B, coronavirus (COV), and Middle East respiratory syndrome coronavirus (MERS COV). This cotton-based testing device potentially exhibits many of the desired characteristics of a suitable point-of-care and portable test, which can be carried out by nurses or clinicians especially for low-resource settings.

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“…FTIR was applied to confirm cellulose hydroxyl groups’ oxidation into aldehyde. 47 Activated cotton swabs were stored for later use.…”

Section: Methodsmentioning

confidence: 99%

Development of a Rapid Immuno-Based Screening Assay for the Detection of Adenovirus in Eye Infections

et al. 2022

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“…Lu et al constructed an aptamer- and AuNPs-based LFS for rapid detection of S. aureus , with a detection time of only 10 min and no cross-reaction with other common bacteria ( Lu et al, 2020a ). Raji et al developed a novel aptamer-based swab sensor for qualitative and quantitative detection of MRSA on environmental surfaces ( Raji et al, 2021 ). In the presence of MRSA on environmental surfaces, MRSA can be combined with aptamers immobilized on cotton swabs and aptamers modified by blue nanobeads to form an “aptamer-MRSA-aptamer” sandwich structure ( Raji et al, 2021 ).…”

Section: Aptasensor For Poctmentioning

confidence: 99%

“…Raji et al developed a novel aptamer-based swab sensor for qualitative and quantitative detection of MRSA on environmental surfaces ( Raji et al, 2021 ). In the presence of MRSA on environmental surfaces, MRSA can be combined with aptamers immobilized on cotton swabs and aptamers modified by blue nanobeads to form an “aptamer-MRSA-aptamer” sandwich structure ( Raji et al, 2021 ). The swab sensor is fast (5 min), sensitive (theoretical value 2 CFU/ml), specific, and can be read directly by the naked eye without the need for laboratory equipment ( Raji et al, 2021 ).…”

Section: Aptasensor For Poctmentioning

confidence: 99%

“…In the presence of MRSA on environmental surfaces, MRSA can be combined with aptamers immobilized on cotton swabs and aptamers modified by blue nanobeads to form an “aptamer-MRSA-aptamer” sandwich structure ( Raji et al, 2021 ). The swab sensor is fast (5 min), sensitive (theoretical value 2 CFU/ml), specific, and can be read directly by the naked eye without the need for laboratory equipment ( Raji et al, 2021 ). This novel aptasensor holds great promise because of its good detection performance, ease of use and relatively inexpensive to produce.…”

Section: Aptasensor For Poctmentioning

confidence: 99%

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Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Chen

Lai

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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The infection of Staphylococcus aureus (S.aureus) and the spread of drug-resistant bacteria pose a serious threat to global public health. Therefore, timely, rapid and accurate detection of S. aureus is of great significance for food safety, environmental monitoring, clinical diagnosis and treatment, and prevention of drug-resistant bacteria dissemination. Traditional S. aureus detection methods such as culture identification, ELISA, PCR, MALDI-TOF-MS and sequencing, etc., have good sensitivity and specificity, but they are complex to operate, requiring professionals and expensive and complex machines. Therefore, it is still challenging to develop a fast, simple, low-cost, specific and sensitive S. aureus detection method. Recent studies have demonstrated that fast, specific, low-cost, low sample volume, automated, and portable aptasensors have been widely used for S. aureus detection and have been proposed as the most attractive alternatives to their traditional detection methods. In this review, recent advances of aptasensors based on different transducer (optical and electrochemical) for S. aureus detection have been discussed in details. Furthermore, the applications of aptasensors in point-of-care testing (POCT) have also been discussed. More and more aptasensors are combined with nanomaterials as efficient transducers and amplifiers, which appears to be the development trend in aptasensors. Finally, some significant challenges for the development and application of aptasensors are outlined.

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“…Recently, cotton swabs have been utilized to develop assays for the detection of various pathogens such as Salmonella typhimurium, 29 Methicillin-resistant Staphylococcus aureus, 30 and Campylobacter jejuni. 31 These detection methods were based on the sandwich immunoassay format using colored beads that lead to color change upon binding with the specific pathogen.…”

Section: Introductionmentioning

confidence: 99%

Combination of Carbon Nanofiber-Based Electrochemical Biosensor and Cotton Fiber: A Device for the Detection of the Middle-East Respiratory Syndrome Coronavirus

2021

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The miniaturization of biosensors for point-of-care diagnosis is highly important in infection control. Electrochemical biosensors offer several advantages in diagnosis in terms of cost, disposability, portability, and sensitivity. Here, a miniaturized electrochemical immunosensor combined with cotton fiber for the detection of the Middle-East respiratory syndrome coronavirus (MERS-CoV) is described. Taking advantage of the absorption capability of cotton, the nasal and saliva samples can be collected and directly transferred to the immunosensor surface for detection using a single tool. The immunosensor was fabricated on a disposable screen-printed electrode precoated with carbon nanofibers. The electrodes were functionalized with carboxyphenyl groups that were used for the immobilization of the spike protein of the MERS-CoV. A competitive detection scheme was employed using the antibody for the MERS-CoV spike protein, and the square-wave voltammetry technique was used for measurements. The biosensor tested after the cotton coating of the electrode exhibited excellent performance. The biosensor was capable of detecting the MERS-CoV spike protein within a concentration range from 0.1 pg•mL −1 to 1 μg•mL −1 with a limit of detection of 0.07 pg•mL, implying the high sensitivity of the method. The immunosensor did not exhibit any cross-reactivity against proteins from HCoV and Influenza A, indicating the excellent selectivity of this approach. Testing of the biosensor in nasal samples showed very high recovery percentages. This disposable biosensor can be used as a miniaturized device for the collection of samples and detection of the virus using a portable potentiostat connected to a smartphone.

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Aptasensor for the detection of Methicillin resistant Staphylococcus aureus on contaminated surfaces

Cited by 19 publications

References 28 publications

Development of a Rapid Immuno-Based Screening Assay for the Detection of Adenovirus in Eye Infections

Development of a Rapid Immuno-Based Screening Assay for the Detection of Adenovirus in Eye Infections

Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Combination of Carbon Nanofiber-Based Electrochemical Biosensor and Cotton Fiber: A Device for the Detection of the Middle-East Respiratory Syndrome Coronavirus

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